紡錘體組裝異常蛋白6抗體費(fèi)用 英文名稱  Anti-SASS6

中文名稱  紡錘體組裝異常蛋白6抗體費(fèi)用 

別    名 HsSAS 6; HsSAS6; MGC119440; SAS 6; SAS6; SASS 6; Spindle assembly 6 homolog (C. elegans); Spindle assembly 6 homolog; Spindle assembly abnormal protein 6 homolog; PJA2_HUMAN.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來(lái)源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Cow, Horse, Sheep

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 免疫學(xué) 細(xì)胞周期蛋白 細(xì)胞分化

蛋白分子量  predicted molecular weight: 74kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human SASS6

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

紡錘體組裝異常蛋白6抗體費(fèi)用 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-F=1:100-500 IF=1:50-200

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 SAS-6 (spindle assembly abnormal protein 6 homolog, HsSAS-6) is a 657 amino acid protein encoded by the human gene SAS6. SAS-6 is a component of the centrosome that contains one PISA (present in SAS-6) domain. LK4, SAS-6, CPAP and other centriole related proteins are required at different stages of procentriole formation and were associated with different centriolar structures. SAS-6 associates only transiently with nascent procentrioles, whereas CEP135 and CPAP form a core structure within the proximal lumen of both parental and nascent centrioles. SAS-6 is necessary for procentriole formation in human cell lines and is localized asymmetrically next to the centriole at the onset of procentriole formation. SAS-6 levels oscillate during the cell cycle; it is degraded in mitosis starting at anaphase, and it accumulates again at the end of the following G1 phase. The anaphase-promoting complex targets SAS-6 for degradation by the 26S Proteasome, and a KEN box in the C-terminus of SAS-6 is necessary for its degradation. Increased SAS-6 levels promoted the formation of multiple procentrioles forming next to a single centriole.

Function : Has E2-dependent E3 ubiquitin-protein ligase activity. Responsible for ubiquitination of cAMP-dependent protein kinase type I and type II-alpha/beta regulatory subunits and for targeting them for proteasomal degradation. Essential for PKA-mediated long-term memory processes.

Subunit : Binds ubiquitin-conjugating enzymes (E2s). In vitro, interacts with the ubiquitin-conjugating enzyme, UBE2D2. The phosphorylated form interacts with PRKAR1A, PRKAR2A and PRKAR2B. Binds the catalytic subunits of cAMP-dependent protein kinase.

Subcellular Location : Cytoplasm. Cell membrane. Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein. Cell junction, synapse (By similarity). Cell junction, synapse, postsynaptic cell membrane, postsynaptic density (By similarity). Note=Localizes at the cytoplasmic side of endoplasmic reticulum and Golgi apparatus. Expressed in the postsynaptic density region of synapses (By similarity). Co-localizes with PRKAR2A and PRKAR2B in the cytoplasm and the cell membrane.

Similarity : Contains 1 RING-type zinc finger.

Database links : UniProtKB/Swiss-Prot: O43164.4

實(shí)驗(yàn)流程：

全、新、優(yōu)、品、好四大特點(diǎn)：

紡錘體組裝異常蛋白6抗體費(fèi)用 全：公司提供上萬(wàn)種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過(guò)程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時(shí)沒(méi)有任何的后顧之憂。

正在熱銷的相關(guān)產(chǎn)品：

改良克氏雙糖鐵2990g用于小腸耶爾森氏菌的鑒定

YT Top Agar 培養(yǎng)基 250g incubation media YT Top Agar 培養(yǎng)基 250g

真線側(cè)耳 亞麻炭疽病原菌。分離源：炭疽病亞麻。 支/瓶

Pick’sBrothBaseA

GVPCLiquidMedium

改良磷酸鹽緩沖液29905250g用于小腸耶爾森氏菌的增菌培養(yǎng)。

2 x YT Agar 培養(yǎng)基 250g incubation media 2 x YT Agar 培養(yǎng)基 250g

植物乳桿菌(胚芽乳桿菌) 分解纖維素 支/瓶

AzideDextroseBroth

VTMBroth

鐵瓊脂250g用于軍團(tuán)菌的選擇性分離培養(yǎng)(ISO標(biāo)準(zhǔn))

MUELLER-HINTON agar .to NCCLS MUELLER-HINTON瓊脂 1.05435.0500 MERCK默克 incubation media MUELLER-HINTON agar .to NCCLS MUELLER-HINTON瓊脂 1.05435.0500 MERCK默克

改良Y培養(yǎng)基 250g 用于小腸耶爾森氏菌的選擇性分離和培養(yǎng)

兔脂肪間質(zhì)干細(xì)胞完全培養(yǎng)基Rabbitadiposederivedmesenchymalstemcellsculturemedium

多粘菌素B 1.2mg/支*5 用1ml無(wú)菌水溶解后添加于100ml HB0256中

CL-0144LoVo(人細(xì)胞)5×106cells/瓶×2

GPT2 Others Rat 大鼠 GPT2 桿狀病毒-昆蟲(chóng)細(xì)胞裂解液 (陽(yáng)性對(duì)照)

人腎系膜細(xì)胞裂解物HRMCL

SK-OV-3[SKOV-3]細(xì)胞，人細(xì)胞 人上皮細(xì)胞,HBE細(xì)胞 腦膜細(xì)胞Many types of cells包裝：5 × 105次方(1ml)

兔角膜前基質(zhì)層成纖維細(xì)胞;RCFBF

TEK Others Human 人 Tie2 / CD202b 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

紡錘體組裝異常蛋白6抗體費(fèi)用CL-0143Lncap clone FGC(人細(xì)胞)5×106cells/瓶×2

CTSZ Others Human 人 CTSZ 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

小鼠肝動(dòng)脈平滑肌細(xì)胞完全培養(yǎng)基 100mL

K7M2 wt [K7M2-WT]小鼠成骨細(xì)胞 K7M2 wt [K7M2-WT] murine osteosarcoma cells DMEM培養(yǎng)基+10%FBS

IL6 Protein Cynomolgus 重組食蟹猴 / 恒河猴 IL6 / Ierleukin-6 蛋白

RL1(大鼠肺成纖維樣細(xì)胞) 5×106cells/瓶×2 JAR(胚絨毛癌細(xì)胞)

技術(shù)外包服務(wù):

★紡錘體組裝異常蛋白6抗體費(fèi)用 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等

★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。