中文名稱  基質(zhì)交感分子1抗體規(guī)格 

英文名稱  Anti-STIM1

別    名 GOK; SIM; STIM 1; STIM1; STIM-1; Stim1 stromal interaction molecule 1; STIM1_HUMAN; STIM1L; Stromal interaction molecule 1.

產(chǎn)品屬性：

基質(zhì)交感分子1抗體規(guī)格 濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit

產(chǎn)品類型   一抗  

研究領(lǐng)域     細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 通道蛋白 細(xì)胞表面分子 淋巴細(xì)胞 t-淋巴細(xì)胞

蛋白分子量  predicted molecular weight: 73kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human STIM1

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

基質(zhì)交感分子1抗體規(guī)格 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:20-100 IF=1:50-200

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1.

Subunit : Forms homooligomers and heterooligomers with STIM2. Interacts with ORAI1. Interacts with MAPRE1; probably required for targeting to the growing microtubule plus ends. Interacts with EFCAB4B/CRACR2A; the interaction is direct and takes place in absence of Ca(2+). Forms a complex with EFCAB4B/CRACR2A and ORAI1 at low concentration of Ca(2+), the complex dissociates at elevated Ca(2+) concentrations. Interacts with TMEM66/SARAF, promoting a slow inactivation of STIM1-dependent SOCE activity, possibly by facilitating the deoligomerization of STIM1.

Subcellular Location : Cell membrane. Endoplasmic reticulum membrane. Cytoplasm > cytoskeleton. Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium. Associated with the microtubule network at the growing distal tip of microtubules.

Tissue Specificity : Ubiquitously expressed in various human primary cells and tumor cell lines.

Similarity : Contains 1 EF-hand domain.

Contains 1 SAM (sterile alpha motif) domain.

實(shí)驗(yàn)流程：

全、新、優(yōu)、品、好四大特點(diǎn)：

基質(zhì)交感分子1抗體規(guī)格 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時(shí)沒有任何的后顧之憂。

技術(shù)外包服務(wù):

★基質(zhì)交感分子1抗體規(guī)格 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等

★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。

正在熱銷的相關(guān)產(chǎn)品：

改良磷酸鹽緩沖液PSB250g用于小腸耶爾森氏菌增菌培養(yǎng)

鹽增菌液(SF) Selenite Enrichment Medium 用于增殖標(biāo)本中的沙門氏菌

脫氫膽酸 Sodium dehydrocholate 25克 BR，98%

改良番茄汁瓊脂培養(yǎng)基250g/瓶用于乳酸菌總數(shù)測(cè)定incubationmedia改良番茄汁瓊脂培養(yǎng)基250g/瓶用于乳酸菌總數(shù)測(cè)定

AcetateAgar

改良綠色酵母菌和真菌肉湯250g用于飲料中真菌及酵母菌檢測(cè)

LES Endo瓊脂 250(g) incubation media LES Endo瓊脂 250(g)

V-P試劑盒(甲、抗酸性染乙液) V-P Reagent(A、B) 10毫升×2 細(xì)菌V-P試驗(yàn)

強(qiáng)化梭菌培養(yǎng)基(RCM)250用于梭菌的分離培養(yǎng)(Merck方法)incubationmedia強(qiáng)化梭菌培養(yǎng)基(RCM)250用于梭菌的分離培養(yǎng)(Merck方法)

3%NaClAlkalinePeptoneWater

WortAgar

m-TGE肉湯 250(g) incubation media m-TGE肉湯 250(g)

山梨醇麥康凱瓊脂平板 Sorbitol Maconkey Agar Plate 10塊

卵黃瓊脂培養(yǎng)基基礎(chǔ)250用于肉毒梭菌、產(chǎn)氣莢膜梭菌的分離培養(yǎng)(GB標(biāo)準(zhǔn))incubationmedia卵黃瓊脂培養(yǎng)基基礎(chǔ)250用于肉毒梭菌、產(chǎn)氣莢膜梭菌的分離培養(yǎng)(GB標(biāo)準(zhǔn))

副溶血性弧菌瓊脂 250g 用于副溶血性弧菌的選擇性分離培養(yǎng)

CL-0151MDA-MB-435S(人導(dǎo)管癌細(xì)胞)5×106cells/瓶×2

CD3E Others Cynomolgus 食蟹猴 CD3e / CD3 epsilon 人細(xì)胞裂解液 (陽性對(duì)照)

小鼠肝動(dòng)脈內(nèi)皮細(xì)胞完全培養(yǎng)基 100mL

HCAEC人內(nèi)皮細(xì)胞 HCAEC in human coronary artery endothelial cells ECM(Sicencell Cat#1001)

IL11 Protein Human 重組人 IL11 / Ierleukin 11 / IL-11 蛋白

RKO-E6(人轉(zhuǎn)基因細(xì)胞) 5×106cells/瓶×2 Jecket(細(xì)胞)

基質(zhì)交感分子1抗體規(guī)格CL-0148MADB106(大鼠癌細(xì)胞)5×106cells/瓶×2

SELL Others Cynomolgus 食蟹猴 CD62L / L-Selectin / SELL 人細(xì)胞裂解液 (陽性對(duì)照)

人上皮細(xì)胞裂解物HPEpiCL

1590細(xì)胞，人癌細(xì)胞系 小鼠,EL-4細(xì)胞 腦平滑肌細(xì)胞Many types of cells包裝：5 × 105次方(1ml)

Beta-TC-6(小鼠瘤胰島β細(xì)胞) 5×106cells/瓶×2

HCASMC Pellet 人平滑肌細(xì)胞團(tuán)塊 > 1 mio.cells 人成纖維細(xì)胞-心室裂解物HCF-av L