全、新、優(yōu)、品、好四大特點(diǎn)：

泛素連接酶Siah1抗體費(fèi)用 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時沒有任何的后顧之憂。

泛素連接酶Siah1抗體費(fèi)用 英文名稱  Anti-SIAH1

中文名稱  泛素連接酶Siah1抗體費(fèi)用 

別    名 hSIAH1; HUMSIAH; Seven in absentia homolog 1 (Drosophila); Seven in absentia homolog 1; Siah 1; Siah 1a; Ubiquitin ligase SIAH1; E3 ubiquitin-protein ligase SIAH1; Seven in absentia homolog 1; Siah-1; Siah-1a.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit

產(chǎn)品類型   一抗  

研究領(lǐng)域     免疫學(xué) 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 泛素

蛋白分子量  predicted molecular weight: 34kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human SIAH1

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

泛素連接酶Siah1抗體費(fèi)用 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Function : E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2 through proteasomal degradation. It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling. Has some overlapping function with SIAH2. Induces apoptosis in cooperation with PEG3. Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus. GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins.

Subcellular Location : Cytoplasm. Nucleus. Predominantly cytoplasmic. Partially nuclear.

Tissue Specificity : Widely expressed at a low level. Down-regulated in advanced hepatocellular carcinomas.

Post-translational modifications : Phosphorylated on Ser-19 by ATM and ATR. This phosphorylation disrupts SIAH1 interaction with HIPK2, and subsequent proteasomal degradation of HIPK2.

Similarity : Belongs to the SINA (Seven in absentia) family.

Contains 1 RING-type zinc finger.

Contains 1 SIAH-type zinc finger.

實(shí)驗(yàn)流程：

正在熱銷的相關(guān)產(chǎn)品：

改良月桂基鹽胰蛋白月示肉湯(MLST)2220g用于奶粉中阪崎腸桿菌的檢驗(yàn)的增菌和分子生物學(xué)檢驗(yàn)方法(PCR)選擇性增菌

彎曲菌無血選擇瓊脂基礎(chǔ) (CM0739) Oxoid incubation media 彎曲菌無血選擇瓊脂基礎(chǔ) (CM0739) Oxoid

威爾酵母 藥用菌 支/瓶

MEE肉湯250g用于腸道菌的選擇性增菌培養(yǎng)(SN標(biāo)準(zhǔn))

十二烷基瓊脂 250g 用于桿菌抗噬菌體培養(yǎng)

甘露醇發(fā)酵培養(yǎng)基24030250g用于鑒別金黃色葡萄球菌發(fā)酵甘露醇試驗(yàn)(化妝品衛(wèi)生規(guī)范微生物檢驗(yàn)方法2007版)。

Lambda Broth 培養(yǎng)基 100g incubation media Lambda Broth 培養(yǎng)基 100g

芽孢桿菌 支/瓶

葡萄糖肉湯250g用于營養(yǎng)苛求菌培養(yǎng)和腸道菌產(chǎn)氣實(shí)驗(yàn)

無淀粉改良羅氏培養(yǎng)基 250g 用于結(jié)核桿菌分離培養(yǎng)

BCYEAgarBase

MRS agar Lactobacillus agar acc.to DEMAN,ROGOSA and SHARPE MRS 1.10660.0500 MERCK默克 incubation media MRS agar Lactobacillus agar acc.to DEMAN,ROGOSA and SHARPE MRS 1.10660.0500 MERCK默克

疊氮化物葡萄糖液態(tài)培養(yǎng)基 250g 用于水和污水中糞性鏈球菌發(fā)酵法的推測試驗(yàn)(CJ/T148-2001和SN/T2206.5-2009)。

SD大鼠骨髓間質(zhì)干細(xì)胞完全培養(yǎng)基SDratbonemarrowmesenchymalstemcellsincompletemedium

MUGNutrientAgar

CL-0184PANC-1(人*癌細(xì)胞)5×106cells/瓶×2

TNK2 Others Human 人 ACK1 / TNK2 桿狀病毒-昆蟲細(xì)胞裂解液 (陽性對照)

人上皮細(xì)胞裂解物HMEpiCL

HCC1428細(xì)胞，人癌細(xì)胞 人小細(xì)胞細(xì)胞,LTEP-P細(xì)胞 晶狀體上皮細(xì)胞Many types of cells包裝：5 × 105次方(1ml)

狗肺成纖維樣細(xì)胞;DogL1

TNFRSF4 Others Human 人 TNFRSF4 / CD134 人細(xì)胞裂解液 (陽性對照)

泛素連接酶Siah1抗體費(fèi)用CL-0183PA317(小鼠成纖維細(xì)胞)5×106cells/瓶×2

SELP Others Human 人 SELP / selectin P / P-selectin 人細(xì)胞裂解液 (陽性對照)

小鼠腸粘膜上皮細(xì)胞完全培養(yǎng)基 100mL

3D4/21豬肺泡巨噬細(xì)胞 Porcine alveolar macrophage 3D4/21 1640+10% FBS+0.1mM NEAA+1mM 酸

CF Protein Mouse 重組小鼠 CF / Ciliary Neuroophic Factor 蛋白 (His 標(biāo)簽)

RBL-1(大鼠嗜堿性粒細(xì)胞性細(xì)胞) 5×106cells/瓶×2 PC-3(人細(xì)胞)

技術(shù)外包服務(wù):

★泛素連接酶Siah1抗體費(fèi)用 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測序、載體構(gòu)建等

★蛋白工程：原核、哺乳動物蛋白表達(dá)系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。