抗體的生物素化標(biāo)記實驗要點：

1. S期激酶相關(guān)蛋白1抗體說明書 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。

產(chǎn)品訂購信息：
英文名稱  Anti-SKP1 

中文名稱   S期激酶相關(guān)蛋白1抗體說明書 

別    名  Cyclin A/CDK2 associated p19; Cyclin A/CDK2 associated protein p19; Cyclin-A/CDK2-associated protein p19; EMC 19; EMC19; OCP 2; OCP II; OCP II protein; OCP-2; OCP-II; OCP2; OCPII; organ of Corti protein 2; Organ of Corti protein II; p19A; p19skp1; RNA polymerase II elongation factor like protein; RNA polymerase II elongation factor like protein OCP2; RNA polymerase II elongation factor-like protein; S phase kinase associated protein 1; S phase kinase associated protein 1A; S phase kinase associated protein 1A p19A; S-phase kinase-associated protein 1; SIII; Skp 1; SKP 1A; skp1; SKP1_HUMAN; SKP1A; TCEB1L; Transcription elongation factor B (SIII) polypeptide 1 like; Transcription Elongation Factor B 1 like; Transcription Elongation Factor B 1-like; Transcription elongation factor B; Transcription elongation factor B SIII polypeptide 1 like.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Sheep 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 免疫學(xué)

蛋白分子量  predicted molecular weight: 19kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human SKP1 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

S期激酶相關(guān)蛋白1抗體說明書 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.

Function : Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.

Subunit : Component of an E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X. Component of the SCF(BTRC) complex, composed of SKP1, CUL1 and BTRC. Part of a SCF(BTRC)-like complex lacking CUL1, which is associated with phosphorylated NFKBIA and RELA; RELA interacts directly with NFKBIA. Component of the SCF(FBXO44) complex, composed of SKP1, CUL1 and FBXO44. Identified in a complex with SKP2, CKS1B and CDKN1B. Interacts with the cyclin A/CDK2 complex. Part of a SCF-like complex consisting of CUL7, RBX1, SKP1 and FBXW8. Part of several SCF complexes containing SKP1 and one of the F-box proteins. Component of a SCF(SKP2)-like complex containing CUL1, SKP1, TRIM21 and SKP2. Interacts with FBXO2, FBXO4, FBXW7 and TRIM21. Interacts with FBXO45 (By similarity). Component of the SCF(FBXO17) complex, composed of SKP1, CUL1 and FBXO17. Component of the SCF(FBXO27) complex, composed of SKP1, CUL1 and FBXO27. Component of the SCF(Cyclin F) complex consisting of CUL1, RBX1, SKP1 and CCNF.

Similarity : Belongs to the SKP1 family.

Database links : UniProtKB/Swiss-Prot: P63208.2

抗體的鑒定：

1）S期激酶相關(guān)蛋白1抗體說明書 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴(kuò)散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。

微管關(guān)聯(lián)蛋白2 單克隆抗體  MAP2 Monoclonal Antibody  700/30ul#1540/100ul#2520/200ul

CD1 單克隆抗體  CD1 Monoclonal Antibody  700/30ul#1540/100ul#2520/200ul

CD2 單克隆抗體  CD2 Monoclonal Antibody  700/30ul#1540/100ul#2520/200ul

CD4 單克隆抗體  CD4 Monoclonal Antibody  700/30ul#1540/100ul#2520/200ul

CD5 單克隆抗體  CD5 Monoclonal Antibody  700/30ul#1540/100ul#2520/200ul

易感基因環(huán)狀結(jié)構(gòu)域蛋白抗體     抑制素結(jié)合蛋白抗體

相關(guān)抗原BRCAA1抗體     抑制素βC抗體

骨髓基質(zhì)干細(xì)胞抗原1抗體     抑制素βB/Inhibin β B抗體

蛋白酪氨酸激酶BAP135抗體     抑制素α/Inhibin α抗體

磷酸化促凋亡Bik蛋白抗體     抑制素A/Inhibin βA抗體

小泡相關(guān)膜蛋白7 多克隆抗體  VAMP7 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

神經(jīng)管畸形相關(guān)蛋白 多克隆抗體  VANG2 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

縫隙樣蛋白 多克隆抗體  VASN Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

小泡胺運輸?shù)鞍?同源物 多克隆抗體  VAT1 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

ATP酶H+轉(zhuǎn)運溶酶體70kDa,V1亞基A 多克隆抗體  VATA Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

地黃 Rehmannia glinosa Libosch. RhMannioside D 地黃苷D 81720-08-3 C27H42O20 ≥98%

苦玄參苷IAPicfqltcrrcqninIcHPLC≥98%，20mg/支

β-桉葉醇;Β-桉葉醇 β-Eudesmol 51317-08-9 20mg HPLC≥98% 用于含量測定

中藥對照藥材桃金娘果121471-200401TLC法鑒別

DECURSIN紫花前胡素純度：≥97%

S期激酶相關(guān)蛋白1抗體說明書大豆Glycinemax(L.)merr Soyasaponin Ba 大豆皂苷Ba 114590-20-4 C48H78O19 ≥98%

原薯蕷皂苷ProtodioscinHPLC≥98%，20mg/支

遠(yuǎn)志皂苷A Onjisaponin A 20mg HPLC≥98% 用于含量測定

對照品新霉胺130411-200307檢查

Cyanidin chloride錄化花青素純度：>98%