全、新、優(yōu)、品、好四大特點(diǎn)：

紡錘體和著絲粒相關(guān)蛋白2抗體費(fèi)用 全：公司提供上萬(wàn)種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

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紡錘體和著絲粒相關(guān)蛋白2抗體費(fèi)用 英文名稱  Anti-SKA2

中文名稱  紡錘體和著絲粒相關(guān)蛋白2抗體費(fèi)用 

別    名 FAM33A; Family with sequence similarity 33, member A; FLJ12758; MGC110975; Protein FAM33A; SKA 2; SKA2; SKA2_HUMAN; Spindle and kinetochore associated complex subunit 2; Spindle and kinetochore associated protein 2; Spindle and kinetochore-associated protein 2; Spindle and KT (kinetochore) associated 2; Spindle and KT associated 2.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來(lái)源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 細(xì)胞周期蛋白 細(xì)胞分化

蛋白分子量  predicted molecular weight: 14kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human SKA2

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

紡錘體和著絲粒相關(guān)蛋白2抗體費(fèi)用 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Ska2 (spindle and kinetochore associated complex subunit 2), also known as FAM33A, is a 121 amino acid component of the Ska1 complex, a microtubule-binding subcomplex of the outer kinetochore that is critical for proper chromosome segregation. The Ska1 complex is a component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. Localized to the outer kinetochore and spindle microtubules during cell proliferation, Ska2 is essential for spindle checkpoint silencing and exit from mitosis. Downregulation of Ska2 leads to delayed recruitment of MAD2, a component of the mitotic spindle checkpoint, to several kinetochores resulting in occasional loss of individual chromosomes from the metaphase plate. Ska2 is encoded by a gene located on human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes.

Function : Component of the SKA1 complex, a microtubule-bindingsubcomplex of the outer kinetochore that is essential for properchromosome segregation. Required for timely anaphase onset duringmitosis, when chromosomes undergo bipolar attachment on spindlemicrotubules leading to silencing of the spindle checkpoint. TheSKA1 complex is a direct component of the kinetochore-microtubuleinterface and directly associates with microtubules as oligomericassemblies. The complex facilitates the processive movement ofmicrospheres along a microtubule in a depolymerization-coupledmanner. In the complex, it is required for SKA1 localization.

Subunit : Component of the SKA1 complex, composed of SKA1, SKA2 andSKA3. Forms a heterodimer with SKA1; the heterodimer interactingwith SKA3. The core SKA1 complex is composed of 2 SKA1-SKA2heterodimers, each heterodimer interacting with a molecule of theSKA3 homodimer. The core SKA1 complex associates with microtubulesand forms oligomeric assemblies. Interacts directly with SKA1.Binds directly to microtubules; but with a much lower affinity thanSKA1. May interact with NR3C1; the relevance of such interactionremains unclear in vivo.

Subcellular Location : Cytoplasm, cytoskeleton, spindle.Chromosome, centromere, kinetochore. Note=Localizes to the outerkinetochore and spindle microtubules during mitosis in a NDC80complex-dependent manner. Localizes to both the mitotic spindle andkinetochore-associated proteins.

Similarity : Belongs to the SKA2 family.

Database links : UniProtKB/Swiss-Prot: Q8WVK7.1

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技術(shù)外包服務(wù):

★紡錘體和著絲粒相關(guān)蛋白2抗體費(fèi)用 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等

★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。