抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 5-羥色胺受體5A抗體說明書 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

產(chǎn)品訂購信息：
英文名稱  Anti-5HT5A receptor/SR-5A 

中文名稱   5-羥色胺受體5A抗體說明書 

別    名  HTR5A; 5 HT 5A; 5 hydroxytryptamine*receptor; 5-HT-5; 5-HT-5A; 5-HT5A; 5-hydroxytryptamine receptor 5A; 5HT5A; 5HT5A_HUMAN; HTR5A; Serotonin receptor 5A.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat 

產(chǎn)品類型   一抗  

研究領(lǐng)域    神經(jīng)生物學(xué) 細(xì)胞膜受體 G蛋白偶聯(lián)受體 G蛋白信號(hào)

蛋白分子量  predicted molecular weight: 40kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human 5HT5A receptor/SR-5A 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

5-羥色胺受體5A抗體說明書 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 SR-5B, also designated 5-hydroxytryptamine (serotonin) receptor 5B (5-HT5B) and G protein-coupled receptor 134 (GPR134) is a receptor for the monoamine ligand serotonin (5-hydroxytryptamine, 5-HT). Serotonin is a neurotransmitter derived from serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin actions are mediated by receptors that influence the biochemistry of depression, anxiety, sexuality and appetite. Rat SR-5B is present in serotonergic neurons in dorsal raphe (DR) and central superior nucleus (CS, median raphe nucleus). DR cell bodies showing SR-5B mRNA expression are abundant in the medial portions of the nucleus. CS coexpression of SR-5B receptor mRNA with serotonin transporter mRNA is high in the intermediate portions of the nucleus. Serotonin receptors include SR-1–7 (5HT1–7). Subtypes within the SR-1 group include SR-1A, -1B, -1D, -1E and -1F. Subtypes within the SR-2 group include SR-2A, -2B and -2C. Subtypes within the SR-5 group include SR-5A and -5B. SR receptors can couple to G proteins that act on either adenylate cyclase or phospholipase C (PLC). The SR-3 class of receptors are ion channels.32234.

Function : This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins.

Subcellular Location : Cell membrane.

Similarity : Belongs to the G-protein coupled receptor 1 family.

Database links : UniProtKB/Swiss-Prot: P47898.1

抗體的鑒定：

1）5-羥色胺受體5A抗體說明書 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力。抗體的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。

泛素關(guān)聯(lián)蛋白2 多克隆抗體  UBAP2 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

泛素結(jié)合酶E2H 多克隆抗體  UBE2H Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

泛素結(jié)合酶E2K 多克隆抗體  UBE2K Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

泛素結(jié)合酶E2O 多克隆抗體  UBE2O Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

泛素結(jié)合酶E2S 多克隆抗體  UBE2S Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

聚集蛋白抗體     增殖相關(guān)蛋白MAGOH抗體

調(diào)亡誘導(dǎo)因子抗體     增殖細(xì)胞核抗原抗體

間變型激酶抗體     增殖潛能相關(guān)蛋白抗體

α-甲基?；o酶A消旋酶抗體     增食欲素B/欲激素B

腺苷單磷酸活化蛋白激酶β1抗體     增食欲素A/欲激素A

內(nèi)皮鈣粘蛋白,VE 小鼠單克隆抗體(8E5)  VE-Cadherin Mouse Monoclonal Antibody(8E5)  700/30ul#1540/100ul#2520/200ul

細(xì)胞外信號(hào)調(diào)節(jié)激酶1 小鼠單克隆抗體(5E9)  ERK1 Mouse Monoclonal Antibody(5E9)  700/30ul#1540/100ul#2520/200ul

細(xì)胞外信號(hào)調(diào)節(jié)激酶1 小鼠單克隆抗體(1C11)  ERK1 Mouse Monoclonal Antibody(1C11)  700/30ul#1540/100ul#2520/200ul

細(xì)胞外信號(hào)調(diào)節(jié)激酶1 小鼠單克隆抗體(4G11)  ERK1 Mouse Monoclonal Antibody(4G11)  700/30ul#1540/100ul#2520/200ul

細(xì)胞外信號(hào)調(diào)節(jié)激酶1/2 小鼠單克隆抗體(1H4)  ERK1/2 Mouse Monoclonal Antibody(1H4)  700/30ul#1540/100ul#2520/200ul

山香圓葉Folium Turpiniae Quercetin 3-O-robinobioside 槲皮素-3-O-洋槐糖苷 52525-35-6 C27H30O16 ≥98%

菊苣醋CichoricccidHPLC≥98%;20mg/支

1,3-二咖非?？鼘幩?洋薊素;洋薊酸;朝鮮薊酸;朝薊素;菜薊素 Cynarin; 1,5-Dicaffeoylinoneic acid 1182-34-9 20mg HPLC≥98% 用于含量測(cè)定

中藥對(duì)照藥材水楊梅根121447-200401TLC法鑒別

Acetyt

5-羥色胺受體5A抗體說明書20(R)人參皂苷Rg3 20(R)Ginsenoside Rg3 分 子 量：785.0230 分子式：C42H72O13 20(R)人參皂苷Rg3--(20(R)Ginsenoside Rg3)的詳細(xì)信息 【提取來源】來源為五加科植物人參Panax ginseng C. A. Mey.的干燥根。 【基本用途】用于含量測(cè)定。

云南百部Stemona mairei Sessilifoline A none 929637-35-4 C22H31NO5 磺安喹林成分分析標(biāo)準(zhǔn)物質(zhì)(8016

檢查用辛可尼丁100211-200502常溫，避光50mg

福辛普利相關(guān)物質(zhì)G標(biāo)準(zhǔn)品15mg

小葉桃花心木Swietenia mahagoni metxyl 6-xy7noxyangolensate none 22255-07-8 C27H34O8 ≥98.5%