產(chǎn)品訂購(gòu)信息：
英文名稱(chēng)  Anti-5HT1F Receptor/SR-1F 

中文名稱(chēng)   5-羥色胺受體1F抗體說(shuō)明書(shū) 

別    名  5 HT 1F; 5 HT1F; 5 hydroxytryptamine (serotonin) receptor 1F; 5 hydroxytryptamine 1F receptor; 5 hydroxytryptamine receptor 1E beta; 5 hydroxytryptamine receptor 1F; 5 hydroxytryptamine serotonin receptor 1E beta; 5 hydroxytryptamine serotonin receptor 1F; 5HT 1F; 5HT1F; HTR 1F; HTR1EB; HTR1EL; HTR1F; MR 77; MR77; Serotonin receptor 1E beta; Serotonin receptor gene; 5HT1F_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來(lái)源   Rabbit

克隆類(lèi)型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep, hu,mo,rat,shp,dog,coe,pig,Rb 

產(chǎn)品類(lèi)型   一抗  

研究領(lǐng)域    神經(jīng)生物學(xué) 細(xì)胞膜受體 G蛋白偶聯(lián)受體 G蛋白信號(hào)

蛋白分子量  predicted molecular weight: 42kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human 5HT1F Receptor/SR-1F 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

5-羥色胺受體1F抗體說(shuō)明書(shū) 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The members of the G-protein-coupled receptor family are distinguished by their slow transmitting response to ligand binding. These seven transmembrane proteins include the adrenergic, serotonin and dopamine receptors. The effect of the signaling molecule can be excitatory or inhibitory depending on the type of receptor to which it binds. b-adrenergic bound to adrenaline activates adenylyl cyclase, while a2-adrenergic receptor bound to adrenaline inhibits adenylyl cyclase. Like the a2-adrenergic receptor, serotonin receptor functions are also mediated by G proteins that inhibit the activity of adenylyl cyclase. The serotonin receptors have been classified into several categories, designated SR-1–7 (5HT1–7). Subtypes within the SR-1 group include SR-1A, -1B, -1D, -1E and -1F.

Function : 5HT1F is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. 5HT1F is a member of the GPCR family (subfamily: Serotonin). Expression of 5HT1F has been reported in various brain structures, including cortex, hippocampus, trigeminal ganglia, and cerebral blood vessels. Expression in peripheral tissues is limited to uterus, mesentery, and artery. No expression has been detected in heart, kidney, liver, pancreas, or spleen.

Subcellular Location : Cell membrane; Multi pass membrane protein.

Similarity : Belongs to the G-protein coupled receptor 1 family.

Database links : UniProtKB/Swiss-Prot: P30939.1

硫氧還蛋白5 多克隆抗體  TXND5 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

酪氨酸羥化酶 多克隆抗體  Tyrosine Hydroxylase Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

含胰蛋白酶域1 多克隆抗體  TYSD1 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

YY1轉(zhuǎn)錄因子 多克隆抗體  TYY1 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

泛素硫酯酶17樣蛋白1 多克隆抗體  U17L1 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

p53凋亡刺激蛋白1抗體     粘著斑激酶抗體

P53凋亡刺激蛋白2抗體     粘著斑蛋白抗體

N-甲基嘌呤DNA糖基化酶     粘膜定居因子抗體

AU5 tag標(biāo)簽抗體     粘膜相關(guān)上皮趨化因子28抗體

鈣離子ATP酶通道蛋白抗體     粘附相關(guān)激酶抗體

抑制蛋白1抗體     5-氨基乙酰合酶1抗體

擴(kuò)張刺激磷蛋白抗體     5’-三磷酸聚核苷酸抗體

囊泡乙酰膽堿通道抗體     4-磷酸磷脂酰肌醇5激酶1樣蛋白抗體

鳥(niǎo)苷酸轉(zhuǎn)換因子VAV1抗體     4號(hào)染色體開(kāi)放閱讀框52抗體

磷酸化鳥(niǎo)苷酸轉(zhuǎn)換因子VAV1抗體     4號(hào)染色體開(kāi)放閱讀框51抗體

青陽(yáng)參Cynanchum otophyllum Qingyangshengenin-3-O- beta-D-cymaropyranoside 青陽(yáng)參苷元-3-O- β-D-磁麻糖 C35H48O11 ≥98%

2”-O-沒(méi)食子酰級(jí)金絲桃苷2"-O-gclloylhypqrinHPLC≥98%;20mg/支

(-)-丁香樹(shù)脂酚-4-O-β-D-芹糖基-(1→2)-β-D-葡萄糖苷 (-)-Syringaresnol-4-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyran...

5-羥色胺受體1F抗體說(shuō)明書(shū)66340-33-8 頭孢噻肟相關(guān)化合物E標(biāo)準(zhǔn)品(NA) 15mg

云南澄廣花Orophea yunnanensis 4-(3,4-二甲氧基本基)-T-3-烯-1-醇 69768-97-4 C12H16O3 ≥95% 醋酸間甲本酯;乙酸間酯0 881-100

L-Carnitine inner salt肉堿20mg/支98%

甲磺酸多拉司瓊相關(guān)雜質(zhì)A標(biāo)準(zhǔn)品25mg甲磺酸多拉司瓊相關(guān)雜質(zhì)A標(biāo)準(zhǔn)品

龍膽苦苷 20831-76-9 HPLC≥98%;20mg 訂購(gòu)|咨詢(xún)

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 5-羥色胺受體1F抗體說(shuō)明書(shū) 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；

3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴(lài)氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）5-羥色胺受體1F抗體說(shuō)明書(shū) 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線(xiàn),計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。