中文名稱  磷酸化嗜中性粒細(xì)胞胞漿因子1抗體說(shuō)明書 

英文名稱  Anti-phospho-NCF1/p47 phox(Ser359)

別    名 phospho-NCF1(Ser359); phospho-p47 phox(Ser359); NCF1 (phospho S359); 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NADPH oxidase organizer 2; NCF 47K; Neutrophil cytosol factor 1; Neutrophil cytosolic factor 1; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox organizing protein 2; NOXO2; p47 phox; p47phox; ;SH3 and PX domain containing protein 1A; SH3PXD1A; NADPH oxidase p47 phox; NCF1_HUMAN.

產(chǎn)品屬性：

磷酸化嗜中性粒細(xì)胞胞漿因子1抗體說(shuō)明書 濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg

抗體來(lái)源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Rat

產(chǎn)品類型   一抗 磷酸化抗體  

研究領(lǐng)域     細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo)

蛋白分子量  predicted molecular weight: 45kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthesised phosphopeptide derived from human NCF1 around the phosphorylation site of Ser359

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

磷酸化嗜中性粒細(xì)胞胞漿因子1抗體說(shuō)明書 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The heredity chronic granulomatous disease (CGF) has been linked to mutations in p47-phox and p67-phox. The cytosolic proteins p47-phox and p67-phox, also designated neutrophil cytosol factor (NCF)1 and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47-phox and p67-phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 domain of p67-phox has been shown to interact with the proline-rich domain of p47-phox, suggesting that p47-phox may faciliate the transport of p67-phox to the membrane.

Function : NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

Subunit : Interacts with NOXA1. Interacts with ADAM15. Interacts with TRAF4. Interacts with FASLG.

Subcellular Location : Cytoplasm.

Post-translational modifications : Phosphorylated by PRKCD; phosphorylation induces activation of NCF1 and NADPH oxidase activity.

DISEASE : Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.

Similarity : Contains 1 PX (phox homology) domain.

Contains 2 SH3 domains.

Database links : UniProtKB/Swiss-Prot: P14598.3

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