產(chǎn)品訂購信息：
英文名稱  Anti-Synaptojanin/INPP5G 

中文名稱   磷酸肌醇磷酸酶蛋白INPP5G抗體品牌 

別    名  5-trisphosphate 5-phosphatase 1; Inositol 5' phosphatase; INPP 5G; INPP5G; KIAA0910; Polyphosphoinositide phosphatase; Synaptic inositol 1 4 5 trisphosphate 5 phosphatase 1; Synaptic inositol 145 trisphosphate 5 phosphatase 1; Synaptic inositol-1 antibody Synaptic inositol-145-trisphosphate 5-phosphatase 1; Synaptojanin 1; Synaptojanin 1 polyphosphoinositide phosphatase; Synaptojanin-1; Synaptojanin1; Synj 1; Synj1; SYNJ1_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo)

蛋白分子量  predicted molecular weight: 173kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human Synaptojanin/INPP5G 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

磷酸肌醇磷酸酶蛋白INPP5G抗體品牌 產(chǎn)品應(yīng)用    ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The inositol polyphosphate 5-phosphatases selectively remove the phosphate from the 5-position of various phosphatidylinositols, which generate second messengers in response to extracellular signals. Synaptojanins are characterized by an N-terminal SAC1-like sequence, a central 5-phosphate domain, and a unique C-terminal sequence and have been shown to use phosphatidylinositol 4,5-bisphosphate as a substrate. Synaptojanins exist as two isoforms, synaptojanin 1 and 2, which differ in the C-terminal domain, and each isoform has multiple variants produced by alternative splicing. Synaptojanin 1 is expressed as two major forms: the shorter is found in brain while the longer is expressed in peripheral tissues. Eight splice variants of synaptojanin 2 have been detected, including a brain specific isoform. Synaptojanins are thought to participate in the endocytosis of synaptic vesicles and the regulation of the actin cytoskeleton.

Function : Inositol 5-phosphatase which has a role in clathrin-mediated endocytosis.

Subunit : Interacts with ASH/GRB2. Interacts with PACSIN1, PACSIN2 and PACSIN3 (By similarity). Binds AMPH, SH3GL1, SH3GL2 and SH3GL3. Interacts with MYO1E (via SH3 domain).

Subcellular Location : Cytoplasm.

Tissue Specificity : Concentrated at clathrin-coated endocytic intermediates in nerve terminals. Isoform 1 is more enriched than isoform 2 in developing brain as well as non-neuronal cells. Isoform 2 is very abundant in nerve terminals.

Similarity : Belongs to the synaptojanin family.

In the central section; belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family.

Contains 1 RRM (RNA recognition motif) domain.

Contains 1 SAC domain.

Database links : UniProtKB/Swiss-Prot: O43426.2

增生型蛋白1 多克隆抗體  TEFF2 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

肌聯(lián)蛋白帽 多克隆抗體  TELT Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

肌腱蛋白R 多克隆抗體  Tenascin-R Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

腱生蛋白N 多克隆抗體  TENN Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

端粒重復(fù)結(jié)合因子2 多克隆抗體  TERF2 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

酸性磷酸酶樣蛋白2抗體     致癌基因n-Myc抗體

AHNAK核蛋白2抗體     致癌基因C-Myc抗體

二磷酸腺苷核糖基化因子6相互作用蛋白抗體     質(zhì)子梯度調(diào)節(jié)蛋白1抗體

腺苷酸激酶1抗體     酯酶抑制蛋白C1IN抗體

頂體前體蛋白抗體     指狀蛋白RET抗體

熒光素酶 小鼠單克隆抗體(9D6)  Luciferase Mouse Monoclonal Antibody(9D6)  700/30ul#1540/100ul#2520/200ul

磷酸酯酶與張力蛋白同源物 小鼠單克隆抗體(2C10)  PTEN Mouse Monoclonal Antibody(2C10)  700/30ul#1540/100ul#2520/200ul

磷酸酯酶與張力蛋白同源物 小鼠單克隆抗體(9E8)  PTEN Mouse Monoclonal Antibody(9E8)  700/30ul#1540/100ul#2520/200ul

細胞周期蛋白B1 小鼠單克隆抗體(5C1)  Cyclin B1 Mouse Monoclonal Antibody(5C1)  700/30ul#1540/100ul#2520/200ul

Epsilon 微管蛋白 小鼠單克隆抗體(3C1)  Epsilon Tubulin Mouse Monoclonal Antibody(3C1)  700/30ul#1540/100ul#2520/200ul

半枝蓮 Poulaca grandiflora Hook Pterisolic acid C none none C20H26O4 ≥95%

去輕二異丁香酚Gotothqxy7nogqntwoisoqugqnol20mg/支

法卡林二醇 Bitte angeben 55297-87-5 50mg HPLC≥98% 法卡林二醇用于含量測定

含量測定阿托伐他汀鈣100590-200902常溫，避光100mg

Danolene丹曲林標(biāo)準品7261-97-4200mg

磷酸肌醇磷酸酶蛋白INPP5G抗體品牌97540-22-2 S-腺苷蛋安酸對甲本磺酸流鹽標(biāo)準品 500mg

云南紅豆杉Taxus yunnanensis 蛻皮甾同-20,22-單丙同化物 22798-96-5 C30H48O7 ≥95% 人胰素(Y0000191

對照品鹽酸扶奮乃靜100162-200302TLC法檢查用

Monowo7ium Glamate (AS)谷安酸一標(biāo)準品142-47-2 1g谷安酸一標(biāo)準品

中華粗榧Cephalotaxus sinensis Angophorol 2,3-二氫-5-羥基-2-(4-羥基本基)-7-甲氧基-6,8-二甲基-4H-1-本并-4-酮 133442-54-3 C18H18O5 ≥95%

抗體的生物素化標(biāo)記實驗要點：

1. 磷酸肌醇磷酸酶蛋白INPP5G抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細胞的熒光標(biāo)記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導(dǎo)致高本底。

抗體的鑒定：

1）磷酸肌醇磷酸酶蛋白INPP5G抗體品牌 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。