抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. α誘導(dǎo)蛋白9/六跨膜上皮抗原4抗體費(fèi)用 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

產(chǎn)品訂購(gòu)信息：
英文名稱  Anti-STEAP4/TNFAIP9 

中文名稱   α誘導(dǎo)蛋白9/六跨膜上皮抗原4抗體費(fèi)用 

別    名  alpha-induced protein 9; Metalloreductase STEAP4; six transmembrane prostate protein 2; Six-transmembrane epithelial antigen of prostate 4; SixTransMembrane protein of prostate 2; STAMP 2; STAMP2; STEA4_HUMAN; STEAP 4; STEAP4; TIARP; TNFAIP 9; TNFAIP9; Tumor necrosis factor; tumor necrosis factor, alpha-induced protein 9; tumor necrosis-alpha-induced adipose-related protein.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit 

產(chǎn)品類型   一抗  

研究領(lǐng)域     免疫學(xué) 細(xì)胞生物標(biāo)志物

蛋白分子量  predicted molecular weight: 52kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human STEAP4 (85-130aa) 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

α誘導(dǎo)蛋白9/六跨膜上皮抗原4抗體費(fèi)用 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor. Play a role in systemic metabolic homeostasis, integrating inflammatory and metabolic responses (By similarity). Associated with obesity and insulin-resistance. Involved in inflammatory arthritis, through the regulation of inflammatory cytokines. Inhibits anchorage-independent cell proliferation.

Function : Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor. Plays a role in systemic metabolic homeostasis, integrating inflammatory and metabolic responses (By similarity). Associated with obesity and insulin-resistance. Involved in inflammatory arthritis, through the regulation of inflammatory cytokines. Inhibits anchorage-independent cell proliferation.

Subunit : Interacts with and phosphorylates PTK2/FAK1.

Subcellular Location : Cell membrane. Golgi apparatus membrane; Multi-pass membrane protein.

Tissue Specificity : Ubiquitous. Highly expressed in adipose tissue. Expressed in placenta, lung, heart and prostate. Detected at lower levels in liver, skeletal muscle, pancreas, testis and small intestine. Highly expressed in joints of patients with rheumatoid arthritis and localized with CD68 cells, a marker for macrophages.

Similarity : Belongs to the STEAP family.

Database links : UniProtKB/Swiss-Prot: Q687X5.1

抗體的鑒定：

1）α誘導(dǎo)蛋白9/六跨膜上皮抗原4抗體費(fèi)用 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力。抗體的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。

褐黑素相關(guān)蛋白ART抗體     周期素D1抗體

腎上腺皮質(zhì)線粒體鐵氧還蛋白抗體     周期素C抗體

芳香烴受體核轉(zhuǎn)錄蛋白樣2抗體     周期素B3抗體

磷酸化芳香胺N-乙酰化轉(zhuǎn)移酶抗體     周期素B2抗體

肌動(dòng)蛋白結(jié)合蛋白1抗體     周期素B1抗體

α誘導(dǎo)蛋白3多克隆抗體  A20 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

AP2關(guān)聯(lián)激酶1多克隆抗體  AAK1 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

含丙氨酰tRNA合成酶域蛋白1多克隆抗體  AARSD1 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

氨基乙二酸半醛脫氫酶磷酸泛酰巰基乙胺轉(zhuǎn)移酶多克隆抗體  AASD-PPT Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

氨基乙二酸半醛合成酶多克隆抗體  AASS Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

人參Panax ginseng C. A. Mey. Protopanaxadiol 原人參二醇 7755--1-3 C30H52O3 ≥98%

槲皮速-7-O-葡萄糖苷#NAMEHPLC≥98%;10mg/支

新穿心蓮內(nèi)酯 Neoandrographolide 27215-14-1 20mg HPLC≥98% 新穿心蓮內(nèi)酯 27215-14-1

含量測(cè)定來扶米特100571-200601常溫，避光100mg

地丹諾辛系統(tǒng)適用性合劑標(biāo)準(zhǔn)品10mg

α誘導(dǎo)蛋白9/六跨膜上皮抗原4抗體費(fèi)用順-7,10,13,16二十二碳四烯酸甲酯 cis-7,10,13,16-Docosateaenoic acid metxyl ester 分 子 量：346.5500 CAS編號(hào)：13487-42-8 分子式：C23H38O2 順-7,10,13,16二十二碳四烯酸甲酯--(cis-7,10,13,16-Docosateaenoic acid metxyl ester )的詳細(xì)信息 操作與貯存：

云南松Pinus yunnanensis metxyl 7,15-dixy7noxydexy7noabietate 7,15-二輕基脫輕樅酸甲酯 155205-65-5 C21H30O4 L-阿拉伯糖

鑒別牡荊油111547-200602常溫，避光0.2ml

Carbidopa卡比多巴標(biāo)準(zhǔn)品38821-49-7400mg

杧果Mangifera indica 27-xy7noxymangiferonic acid 27-羥基杧果酮酸 5132-66-1 C30H46O4 ≥95%