產(chǎn)品訂購信息：
英文名稱  Anti-SNAPIN/SNAP25BP 

中文名稱   突觸相關(guān)蛋白25結(jié)合蛋白抗體費(fèi)用 

別    名  SNAP associated protein; SNAP-25-binding protein; SNAP-associated protein; SNAP25BP; SNAPAP; SNAPIN; SNAPN_HUMAN; SNARE associated protein snapin; SNARE-associated protein Snapin; Synaptosomal associated protein 25 binding protein; Synaptosomal-associated protein 25-binding protein; OTTHUMP00000035157.

濃    度   1mg/1ml

規(guī) 格  0.1ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 細(xì)胞類型標(biāo)志物

蛋白分子量  predicted molecular weight: 15kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human SNAP25BP 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

突觸相關(guān)蛋白25結(jié)合蛋白抗體費(fèi)用 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The protein encoded by this gene is a coiled-coil-forming protein that associates with the SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) complex of proteins and the BLOC-1 (biogenesis of lysosome-related organelles) complex. Biochemical studies have identified additional binding partners. As part of the SNARE complex, it is required for vesicle docking and fusion and regulates neurotransmitter release. The BLOC-1 complex is required for the biogenesis of specialized organelles such as melanosomes and platelet dense granules. Mutations in gene products that form the BLOC-1 complex have been identified in mouse strains that are models of Hermansky-Pudlak syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012].

Function : May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release by potentiating the interaction of synaptotagmins with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells.

Subunit : Interacts with CSNK1D, SNAP23 and STX4A but not with STX1A, VAMP2 and SYT1. Interacts with SNAP25; the interaction with SNAP25 is increased by its phosphorylation (By similarity). Component of the biogenesis of lysosome-related organelles complex 1 (BLOC-1) composed of BLOC1S1, BLOC1S2, BLOC1S3, BLOC1S4, BLOC1S5, BLOC1S6, DTNBP1/BLOC1S7 and SNAPIN/BLOC1S8. Octamer composed of one copy each BLOC1S1, BLOC1S2, BLOC1S3, BLOC1S4, BLOC1S5, BLOC1S6, DTNBP1/BLOC1S7 and SNAPIN/BLOC1S8. The BLOC-1 complex associates with the AP-3 protein complex and membrane protein cargos. Associates with the SNARE complex. Interacts with CEP110, NANOS1, PUM2 and RGS7. Interacts with human cytomegalovirus/HHV-5 protein UL70.

Subcellular Location : Cytoplasm (By similarity). Membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Cell junction, synapse, synaptosome. Cytoplasm, perinuclear region. Golgi apparatus membrane (By similarity). Note=May be cytoplasmic and peripheral membrane bound or anchored to the vesicular membrane through an N-terminal signal anchor (By similarity). Co-localizes with NANOS1 and PUM2 in the perinuclear region of germ cells.

Tissue Specificity : Expressed in male germ cells of adult testis (at protein level).

Post-translational modifications : Phosphorylated by CSNK1D/CK1 (By similarity).

Similarity : Belongs to the SNAPIN family.

Database links : UniProtKB/Swiss-Prot: O95295.1

痕量胺關(guān)聯(lián)受體6 多克隆抗體  TAAR6 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

痕量胺相關(guān)受體8 多克隆抗體  TAAR8 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

痕跡胺關(guān)聯(lián)受體9 多克隆抗體  TAAR9 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

TAK1結(jié)合蛋白3 多克隆抗體  TAB3 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

TARDNA結(jié)合蛋白 多克隆抗體  TADBP Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

淀粉樣蛋白β前體樣蛋白1抗體     /抗原74抗體

α2巨球蛋白(α-2M)抗體     /抗原65

ACCSL蛋白抗體     /抗原44抗體

轉(zhuǎn)錄激活蛋白crsp70抗體     /抗原3抗體

粘附分子IgG樣結(jié)構(gòu)域蛋白1抗體     /抗原2抗體

原鈣粘附蛋白α9抗體     PUM2蛋白抗體

原鈣粘附蛋白α7抗體     PUM1蛋白抗體

原鈣粘附蛋白α8抗體     PSP抗體

鈣粘蛋白相關(guān)神經(jīng)受體8抗體     PSF2蛋白抗體

原鈣粘蛋白15抗體     PSD95相關(guān)緊密連接蛋白PATJ抗體

土荊皮Coex Pseudolaricis Pseudolaric Acid C 土荊皮 82601-41-0 C21H26O7 ≥98%

科羅索醋CorosolicccidHPLC≥98%;20mg/支

遠(yuǎn)志皂苷元 Senegenin 2469-34-3 20mg/支 HPLC≥98% 用于含量測定

含量測定尼群地平100585- 201003 常溫，避光100mg

Monoglycerides單甘酯標(biāo)準(zhǔn)品68990-53-4 125mg

突觸相關(guān)蛋白25結(jié)合蛋白抗體費(fèi)用5538-41-0 錄兒價(jià)分相關(guān)物質(zhì)A標(biāo)準(zhǔn)品 25mg

云南蘿芙木Rauvolfia yunnanensis 北升寧 142542-89-0 C14H20O8 ≥98.5% 異環(huán)靈酰安 雜質(zhì)B(I0060004

萎銹靈標(biāo)準(zhǔn)品 環(huán)丙酰安標(biāo)準(zhǔn)品 聯(lián)本進(jìn)酯標(biāo)準(zhǔn)品

Calcium Ascorbate抗壞血酸鈣標(biāo)準(zhǔn)品5743-28-2200mg抗壞血酸鈣標(biāo)準(zhǔn)品

阿魏酸乙酯 4046-02-0 HPLC≥98%;20mg 訂購|咨詢

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 突觸相關(guān)蛋白25結(jié)合蛋白抗體費(fèi)用 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）突觸相關(guān)蛋白25結(jié)合蛋白抗體費(fèi)用 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗(yàn)測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。