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產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
品牌 | 上海莼試 |
保存條件 | Store at -20 °C |
貨號(hào) | CS12159 |
應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
CAS編號(hào) | |
抗體名 | Anti-SPARCL1/Ecm2 |
克隆性 | 是 |
靶點(diǎn) | 詳見說明書 |
適應(yīng)物種 | 詳見說明書 |
形態(tài) | 詳見說明書 |
宿主 | 詳見說明書 |
亞型 | IgG |
標(biāo)識(shí)物 | 詳見說明書 |
濃度 | 1mg/1ml% |
免疫原 | KLH conjugated synthetic peptide derived from human SPARCL1/Ecm2 |
技術(shù)外包服務(wù):
★細(xì)胞外基質(zhì)蛋白2抗體品牌 分子生物學(xué):質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué):基因合成、引物合成、基因測(cè)序、載體構(gòu)建等
★蛋白工程:原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等
★病毒包裝:腺病毒、慢病毒等
★抗體工程:磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等
★細(xì)胞工程:細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。
實(shí)驗(yàn)流程:
細(xì)胞外基質(zhì)蛋白2抗體品牌 英文名稱 Anti-SPARCL1/Ecm2
中文名稱 細(xì)胞外基質(zhì)蛋白2抗體品牌
別 名 Ecm2; RAGS1 antigen; Extracellular matrix protein 2; Hevin; High endothelial venule protein; MAST 9; mast9; Matrix glycoprotein Sc1; PIG33; proliferation inducing protein 33; SC1; SPARC like 1; SPARC-like protein 1; Sparcl1; SPRL1_HUMAN.
產(chǎn)品屬性:
濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Pig, Horse, Rabbit
產(chǎn)品類型 一抗
研究領(lǐng)域 鋅指蛋白
蛋白分子量 predicted molecular weight: 74kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human SPARCL1/Ecm2
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
細(xì)胞外基質(zhì)蛋白2抗體品牌 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 SPARC (secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. SPARC-like protein 1 (SPARCL1), also known as high endothelial venule protein (Hevin) or MAST9, is a 664 amino acid member of the SPARC family of proteins. Highly expressed in lymph node, heart, lung, brain, skeletal muscle, ovary, colon and small intestine, SPARCL1 is a secreted protein that contains one EF-hand domain, one follistatin-like domain and one Kazal-like domain. SPARCL1 is implicated to play a role in neuronal remodeling and tumor suppression. The gene encoding SPARCL1 maps to chromosome 4q22.1.
Subcellular Location : Secreted, extracellular space, extracellular matrix.
Tissue Specificity : Highly expressed in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon, with lower levels in placenta, pancreas, testis, spleen, and thymus, and no expression in kidney, liver, and peripheral blood leukocytes.
Post-translational modifications : N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. Thr-398 is the preferred site to Thr-399.
Similarity : Belongs to the SPARC family.
Contains 1 EF-hand domain.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain.
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