產(chǎn)品訂購信息：
英文名稱  Anti-SAP62 

中文名稱   剪接體相關(guān)蛋白62抗體品牌 

別    名  PRPF11; SF3a66; SAP 62; SAP-62; Spliceosome-associated protein 62; splicing factor 3a subunit 2; SF3A2_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Cow 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細胞生物 表觀遺傳學(xué)

蛋白分子量  predicted molecular weight: 49kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human SAP62 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

剪接體相關(guān)蛋白62抗體品牌 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 SAP 62, also known as SF3A2 (Splicing factor 3A subunit 2), PRP11, PRPF11 or SF3a66, is a 464 amino acid protein that contains one matrin-type zinc finger and belongs to the SF3A2 family. Localized to the nucleus, SAP 62 is a subunit of the SF3A splicing factor, a heterotrimeric complex comprised of three subunits that act in tandem to mediate the binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. The SF3A complex is necessary for the conversion of 15S U2 snRNP into the active 17S protein that performs directly in pre-mRNA splicing events. Functioning as the second subunit of the complex, SAP 62 interacts with subunit 1 (SAP 114) via its N-terminus while simultaneously binding to 15S U2 snRNP via its zinc finger domain. In addition to its role in RNA splicing, SAP 62 is thought to act independently as a microtubule-binding protein.

Function : Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.

Subunit : Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62, SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Identified in the spliceosome C complex. Interacts with HTATSF1.

Subcellular Location : Nuclear

Tissue Specificity : Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62, SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Identified in the spliceosome C complex. Interacts with HTATSF1.

Similarity : Belongs to the SF3A2 family.

Contains 1 matrin-type zinc finger.

Database links : UniProtKB/Swiss-Prot: Q15428.2

RIO激酶2 多克隆抗體  RIOK2 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

受體(TNFRSF)結(jié)合絲氨酸/蘇氨酸激酶1 多克隆抗體  RIPK1 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

受體(TNFRSF)結(jié)合絲氨酸/蘇氨酸激酶2(磷酸化-Ser176) 多克隆抗體  RIPK2 (Phospho-Ser176) Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

受體(TNFRSF)結(jié)合絲氨酸/蘇氨酸激酶3 多克隆抗體  RIPK3 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

GTP結(jié)合蛋白RIT2 多克隆抗體  RIT2 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

衰老相關(guān)蛋白5抗體     組蛋白乙酰轉(zhuǎn)移酶PCAF抗體

釉基質(zhì)蛋白抗體     組蛋白乙酰轉(zhuǎn)移酶MOF抗體

磷酸化腺苷單磷酸活化蛋白激酶α2抗體     組蛋白相關(guān)Bmi1抗體

磷酸化雄激素受體抗體     組蛋白替換精蛋白DGGR1抗體

錨定蛋白重復(fù)結(jié)構(gòu)域蛋白激酶ANKK1抗體     組蛋白去乙?；?抗體

組蛋白去乙酰化酶3 多克隆抗體  HDAC3 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

組蛋白去乙?；? 多克隆抗體  HDAC4 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

組蛋白去乙?；? 多克隆抗體  HDAC5 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

組蛋白去乙?；?/9 多克隆抗體  HDAC5/9 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

組蛋白去乙酰化酶6 多克隆抗體  HDAC6 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

車前草Plantagodepressa Willd Plantamajoside 大車前苷 104777-68-6 C29H36O16 ≥98%

去異波爾定Norisoboldinq10mg/支

7-安基-4-甲基 7-aMino-4-metxylcouMarin 26093-31-2 HPLC≥98% HPLC

含量測定異丙托溴胺100522-200601常溫，避光100mg

Ioxaglic Acid典克沙酸標(biāo)準(zhǔn)品59017-64-0 100mg

剪接體相關(guān)蛋白62抗體品牌NA 錄喹相關(guān)化合物E標(biāo)準(zhǔn)品(NA) 15mg

柘Cudrania icuspidata 香橙素，香樹素 480-20-6 C15H12O6 ≥98.5% 水楊酸甲酯190 7-100

8-Epicrepiside E 93395-30-3

Chloroxyleol錄兒價分標(biāo)準(zhǔn)品1988-4-6125mg錄兒價分標(biāo)準(zhǔn)品

羊藿素 HPLC≥98%;20mg 訂購|咨詢

抗體的生物素化標(biāo)記實驗要點：

1. 剪接體相關(guān)蛋白62抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細胞的熒光標(biāo)記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導(dǎo)致高本底。

抗體的鑒定：

1）剪接體相關(guān)蛋白62抗體品牌 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。