全、新、優(yōu)、品、好四大特點(diǎn)：

神經(jīng)遷移蛋白Slit2/3抗體說明書 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時(shí)沒有任何的后顧之憂。

神經(jīng)遷移蛋白Slit2/3抗體說明書 英文名稱  Anti-SLIT2/Slil3

中文名稱  神經(jīng)遷移蛋白Slit2/3抗體說明書 

別    名 Drad-1; E030015M03Rik; E130320P19Rik; FLJ14420; Slil 3; Slil3; Slit 2; Slit homolog 2 (Drosophila); Slit homolog 2; Slit homolog 2 protein.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 結(jié)合蛋白

蛋白分子量  predicted molecular weight: 170kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human Slit2 C-terminus

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

神經(jīng)遷移蛋白Slit2/3抗體說明書 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Slit proteins are ligands at the Roundabout (Robo) receptors and act as guidance cues in axonal migration/navigation during neural development, at the ventral midline of the neural tube. Slit1 and Slit2 are essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb. A number of cleavage products are reported in the literature for Slit2 protiens (following alternate splicing). The C-terminal cleavage proteins are more diffusible than the larger N-terminal protein that is more tightly cell associated. Slit2 protein is expressed in fetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea.

Function : Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.

Subcellular Location : Secreted. The C-terminal cleavage protein is more diffusible than the larger N-terminal protein that is more tightly cell associated.

Tissue Specificity : Fetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea and other tissues examined.

Similarity : Contains 1 CTCK (C-terminal cystine knot-like) domain.

Contains 7 EGF-like domains.

Contains 1 laminin G-like domain.

Contains 20 LRR (leucine-rich) repeats.

Contains 4 LRRCT domains.

Contains 4 LRRNT domains.

實(shí)驗(yàn)流程：

正在熱銷的相關(guān)產(chǎn)品：

GR ELISA Kit 大鼠糖皮質(zhì)類固醇受體Multi-class antibodies規(guī)格： 48T

Anti-Phospho-Tuberin/TSC2 (Ser1387) 磷酸化馬鈴薯球蛋白(性硬化)抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh GDNF Receptor alpha 2 膠質(zhì)細(xì)胞系源性神經(jīng)營(yíng)養(yǎng)因子受體α2抗體 規(guī)格 0.1ml

DSIP(Human delta sleep-inducing peptide) ELISA Kit 人促睡眠肽 96T

Neurokin B receptor 英文名稱： 神經(jīng)激肽B受體抗體 0.2ml

ADAM2 英文名稱： 去整合素樣金屬蛋白酶2抗體 0.1ml

Anti-Phospho-Tuberin/TSC2 (Ser1387) 磷酸化馬鈴薯球蛋白(性硬化)抗體Multi-class antibodies規(guī)格： 0.1ml

Anti-Dectin-1/CLECSF12/RBITC 紅色熒光素羅丹明標(biāo)記C型凝集素結(jié)構(gòu)域家族7成員A抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-ERK1/MAPK3/FITC 熒光素標(biāo)記原活化蛋白激酶3抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Calcitonin 降鈣素抗體 規(guī)格 0.1ml

MEK1/MAPKK1(mitogen activated protein kinase kinase 1) MEK1/MAPKK1抗體(N-端) 0.5mg

HRASLS2 英文名稱： HRAS樣抑制因子2抗體 0.1ml

Rhesus antibody Rh SERPIN A11 絲氨酸蛋白酶抑制劑A11抗體 規(guī)格 0.2ml

Anti-ERK1/MAPK3/FITC 熒光素標(biāo)記原活化蛋白激酶3抗體IgGMulti-class antibodies規(guī)格： 0.2ml

GKRP(Human glucokinase regulatory protein) ELISA Kit 人葡萄糖激酶調(diào)節(jié)蛋白Multi-class antibodies規(guī)格： 48T

Anti-MCM2 染色體維持缺陷蛋白2抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh JMJD6 凋亡細(xì)胞清除受體抗體 規(guī)格 0.2ml

ANG ELISA Kit 大鼠生長(zhǎng)素 96T

phospho-PKC Epsilon(Ser729) 英文名稱： 磷酸化蛋白激酶C/p-PKC ε抗體 0.1ml

C3orf20 英文名稱： 3號(hào)染色體開放閱讀框20抗體 0.2ml

Anti-MCM2 染色體維持缺陷蛋白2抗體Multi-class antibodies規(guī)格： 0.2ml

STK4 Others Human 人 STK4 / MST1 桿狀病毒-昆蟲細(xì)胞裂解液 (陽性對(duì)照)

人腦干星形膠質(zhì)細(xì)胞HA-bs

猞猁皮膚成纖維樣細(xì)胞;ELS1

BALB/3T3細(xì)胞，BALB/C小鼠胚成纖維細(xì)胞 人*癌細(xì)胞,PC-2細(xì)胞 CM-M024小鼠淋巴成纖維細(xì)胞完全培養(yǎng)基100mL

大鼠細(xì)胞;UMR-106

CDH1 Others Human 人 Cadherin-1 / CDH1 / CD324 / E-cadherin 人細(xì)胞裂解液 (陽性對(duì)照)

CL-0367IM-9(人外周血B淋巴細(xì)胞)5×106cells/瓶×2

CD58 Others Human 人 LFA-3 / CD58 人細(xì)胞裂解液 (陽性對(duì)照)

人平滑肌細(xì)胞總RNAHPSMC NA

MDA-MB-231細(xì)胞，人癌細(xì)胞 BALB/c小鼠ES細(xì)胞,B/c-ES細(xì)胞 BPH-1, 人細(xì)胞

NCI-H1975(人) 5×106cells/瓶×2

HDMEC adult Pellet 人真皮微內(nèi)皮細(xì)胞團(tuán)塊(成年捐獻(xiàn)者) > 1 mio.cells 小氣道上皮細(xì)胞培養(yǎng)基SAEpiCM-prf

神經(jīng)遷移蛋白Slit2/3抗體說明書 CL-0365HuTu-80(人十二脂腸腺癌)5×106cells/瓶×2

MRPL44 Others Human 人 MRPL44 桿狀病毒-昆蟲細(xì)胞裂解液 (陽性對(duì)照)

人上皮細(xì)胞總RNAHPEpiC NA

綿羊肺細(xì)胞;OAR-L1 胚胎成纖維細(xì)胞，NIH/3T3細(xì)胞 NCTC1469細(xì)胞，小鼠正常肝細(xì)胞

人胚胎皮膚成纖維細(xì)胞;HES

HA Others H11N9 甲型 H11N9 (A/mallard/Alberta/294/1977) 血凝素HA1 (Hemagglutinin) 人細(xì)胞裂解液 (陽性對(duì)照)

技術(shù)外包服務(wù):

★神經(jīng)遷移蛋白Slit2/3抗體說明書 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等

★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。