抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 染色體結(jié)構(gòu)維持蛋白4抗體規(guī)格 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；

3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

產(chǎn)品訂購(gòu)信息：
英文名稱  Anti-SMC4 

中文名稱   染色體結(jié)構(gòu)維持蛋白4抗體規(guī)格 

別    名  Chromosome associated polypeptide C; hCAP C; XCAP C homolog; CAP C; CAPC; SMC 4; SMC4; SMC4L1; Structural maintenance of chromosomes 4 like 1; SMC4_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來(lái)源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 細(xì)胞周期蛋白 細(xì)胞分化 表觀遺傳學(xué)

蛋白分子量  predicted molecular weight: 147kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from hu SMC4 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

染色體結(jié)構(gòu)維持蛋白4抗體規(guī)格 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 SMC4 is a central component of the condensin complex, which is required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.

Function : Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.

Subunit : Forms a heterodimer with SMC2. Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: BRRN1/CAPH, CNAP1/CAPD2 and CAPG.

Subcellular Location : Nucleus. Cytoplasm. Chromosome. Note=In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.

Tissue Specificity : Widely expressed. Higher expression in testis, colon, thymus.

Similarity : Belongs to the SMC family. SMC4 subfamily.

Database links : UniProtKB/Swiss-Prot: Q9NTJ3.2

抗體的鑒定：

1）染色體結(jié)構(gòu)維持蛋白4抗體規(guī)格 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。

Anti-Mcpt7/Tryptase Beta1/FITC 熒光素標(biāo)記兔抗人、大、小鼠肥大細(xì)胞蛋白酶7抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Phospho-TIF1beta (Ser824)/FITC 熒光素標(biāo)記磷酸化轉(zhuǎn)錄中介因子Tif1β抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh ACOX1 過(guò)氧化物酶?；o酶A氧化酶1抗體 規(guī)格 0.2ml

Rabbit Anti-Bov IgG/PE PE標(biāo)記的兔抗IgG 0.1ml

Gemin 4 英文名稱： 脊髓性肌癥蛋白Gemin4抗體 0.2ml

Rhesus antibody Rh Rabbit Anti-Biotin/PE-Cy7 PE-Cy7標(biāo)記的兔抗生物素抗體IgG 規(guī)格 0.1ml

Anti-Phospho-TIF1beta (Ser824)/FITC 熒光素標(biāo)記磷酸化轉(zhuǎn)錄中介因子Tif1β抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-DPV/FITC 熒光素標(biāo)記抗鴨瘟病毒抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Mcpt7/Tryptase Beta1/FITC 熒光素標(biāo)記兔抗人、大、小鼠肥大細(xì)胞蛋白酶7抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh CA125/MUC16 抗原抗體 規(guī)格 0.1ml

ChRM3 peptide 毒蕈堿型乙酰膽堿受體M3抗原 0.5mg

HS6ST1 英文名稱： 乙酰肝素6腦苷脂轉(zhuǎn)酶1抗體 0.2ml

Rhesus antibody Rh SECTM1 分泌和跨膜蛋白1抗體 規(guī)格 0.2ml

Anti-Mcpt7/Tryptase Beta1/FITC 熒光素標(biāo)記兔抗人、大、小鼠肥大細(xì)胞蛋白酶7抗體IgGMulti-class antibodies規(guī)格： 0.2ml

IDUA(Human Alpha-L-iduronidase) ELISA Kit 人αL艾杜糖苷酸酶Multi-class antibodies規(guī)格： 48T

Anti-NKB 神經(jīng)激肽B抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh IL-13 白介素13抗體 規(guī)格 0.1ml

17-OHCS ELISA Kit 大鼠17羥皮質(zhì)類固醇 96T

phospho-PIK3R1(Tyr368) 英文名稱： 磷酸化磷脂酰肌醇激酶抗體 0.1ml

CCL27/CTACK 英文名稱： 皮膚T細(xì)胞虜獲趨化因子抗體 0.1ml

Anti-NKB 神經(jīng)激肽B抗體Multi-class antibodies規(guī)格： 0.2ml

NCR1 Others Rat 大鼠 NCR1 / NK-p46 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

BALB/C小鼠肝瘤株;BNL 1ME A.7R.1

原代神經(jīng)膠質(zhì)細(xì)胞特制無(wú)血清添加劑Many types of cells包裝：1ml

ECV-304細(xì)胞，人臍內(nèi)皮(具有T24細(xì)胞特性) 金黃色葡萄球菌 人牙齦成纖維細(xì)胞總RNAHGF NA

RAG(小鼠腎腺癌細(xì)胞) 5×106cells/瓶×2

WB-F344(大鼠肝上皮樣干細(xì)胞) 5×106cells/瓶×2 CL-0271D283 Med(人腦髓母細(xì)胞瘤細(xì)胞)5×106cells/瓶×2 豹貓肺成纖維樣細(xì)胞;LCL3

CL-0338G1(發(fā)綠色熒光的小鼠胚胎干細(xì)胞)5×106cells/瓶×2

ESAM Others Mouse 小鼠 ESAM / Endothelial Cell Adhesion Molecule 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

小鼠肺大動(dòng)脈內(nèi)皮細(xì)胞完全培養(yǎng)基 100mL

Eca-109人細(xì)胞 Human esophageal cancer cell line Eca-109. 1640+10% FBS

IL1B Protein Rat 重組大鼠 IL-1 beta / IL1B 蛋白 (mature form)

NCI-H524(人非小細(xì)胞細(xì)胞) 5×106cells/瓶×2 HUVEC(人臍內(nèi)皮細(xì)胞)

染色體結(jié)構(gòu)維持蛋白4抗體規(guī)格 CL-0336FC33(人胚胎腎細(xì)胞(Asp-2基因修飾))5×106cells/瓶×2

CS Others Human 人 Ciate syhase / CS 桿狀病毒-昆蟲(chóng)細(xì)胞裂解液 (陽(yáng)性對(duì)照)

人腸平滑肌細(xì)胞總RNAHISMC NA

山羊皮膚細(xì)胞;WGS1 大鼠心肌細(xì)胞，H9c2細(xì)胞 Ca759細(xì)胞，615小鼠株

人胚胎成纖維樣細(xì)胞;HEH2

HA Others H1N2 甲型 H1N2 (A/swine/Guangxi/13/2006) 血凝素HA1 (Hemagglutinin) 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)