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粘結(jié)蛋白SA2抗體說明書
  • 品牌:上海莼試
  • 產(chǎn)地:進(jìn)口、國產(chǎn)
  • 貨號(hào):CS12084
  • 發(fā)布日期: 2019-02-13
  • 更新日期: 2025-03-14
產(chǎn)品詳請
產(chǎn)地 進(jìn)口、國產(chǎn)
品牌 上海莼試
保存條件 Store at -20 °C
貨號(hào) CS12084
應(yīng)用范圍 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
CAS編號(hào)
抗體名 Anti-SA2
克隆性
靶點(diǎn) 詳見說明書
適應(yīng)物種 詳見說明書
形態(tài) 詳見說明書
宿主 詳見說明書
亞型 IgG
標(biāo)識(shí)物 詳見說明書
濃度 1mg/1ml%
免疫原 KLH conjugated synthetic peptide derived from human SA2

全、新、優(yōu)、品、好四大特點(diǎn):

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粘結(jié)蛋白SA2抗體說明書 英文名稱  Anti-SA2

中文名稱  粘結(jié)蛋白SA2抗體說明書 

     Cohesin Subunit SA 2; Cohesin subunit SA-2; DKFZp686P168; DKFZp781H1753; SA 2; SA2; SCC3 homolog 2; STAG 2; STAG 2; stag2; STAG2_HUMAN; Stromal antigen 2; FLJ25871; bA517O1.1.

產(chǎn)品屬性:

      1mg/1ml

規(guī)   0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 細(xì)胞周期蛋白 細(xì)胞分化 表觀遺傳學(xué)

蛋白分子量  predicted molecular weight: 141kDa 

       Lyophilized or Liquid

  KLH conjugated synthetic peptide derived from human SA2

      IgG

純化方法   affinity purified by Protein A

儲(chǔ)    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

粘結(jié)蛋白SA2抗體說明書 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

(石蠟切片需做抗原修復(fù)) 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.

Function : Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.

Subunit : Interacts directly with RAD21 in cohesin complex. Cohesin complexes are composed of a heterodimer between a SMC1 protein (SMC1A or SMC1B) and SMC3, which are attached via their hinge domain, and RAD21 which link them at their heads, and one STAG protein (STAG1, STAG2 or STAG3). In cohesin complexes, STAG2 is mutually exclusive with STAG1 and STAG3.

Subcellular Location : Nucleus. Chromosome. Chromosome, centromere. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK1, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex.

Post-translational modifications : Phosphorylated by PLK1. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation (By similarity).

Similarity : Belongs to the SCC3 family.

Contains 1 SCD (stromalin conservative) domain.

Database links : UniProtKB/Swiss-Prot: Q8N3U4.3

實(shí)驗(yàn)流程:

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技術(shù)外包服務(wù):

粘結(jié)蛋白SA2抗體說明書 分子生物學(xué):質(zhì)粒抽提、PCRQ-PCR、RT-PCR、分子生物學(xué):基因合成、引物合成、基因測序、載體構(gòu)建等

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病毒包裝:腺病毒、慢病毒等

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