抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 磷酸化神經(jīng)生長相關(guān)蛋白SCG10抗體說明書 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時,或位于酶的催化位點(diǎn)時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。

產(chǎn)品訂購信息：
英文名稱  Anti-phospho-STMN2/SCG10 (Ser50) 

中文名稱   磷酸化神經(jīng)生長相關(guān)蛋白SCG10抗體說明書 

別    名  Neuronal growth associated protein; SCG 10; SCG10; SCG10 protein; SCGN 10; SCGN10; SGC 10; SGC10; Stathmin 2; Stathmin like 2; STMN 2; STMN-2; Superior cervical ganglia neural specific 10; Superior cervical ganglion 10 protein; Superiorcervical ganglia neural specific 10.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Horse, Rabbit 

產(chǎn)品類型   一抗 磷酸化抗體  

研究領(lǐng)域    細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué)

蛋白分子量  predicted molecular weight: 20kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human STMN2 around the phosphorylation site of Ser50 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化神經(jīng)生長相關(guān)蛋白SCG10抗體說明書 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 This gene encodes a member of the stathmin family of phosphoproteins. Stathmin proteins function in microtubule dynamics and signal transduction. The encoded protein plays a regulatory role in neuronal growth and is also thought to be involved in osteogenesis. Reductions in the expression of this gene have been associated with Down's syndrome and Alzheimer's disease. Alternatively spliced transcript variants have been observed for this gene. A pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Nov 2010].

Function : Regulator of microtubule stability. When phosphorylated by MAPK8, stabilizes microtubules and consequently controls neurite length in cortical neurons. In the developing brain, negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone.

Subunit : Interacts with MAPK8. Interacts with ITM2C.

Subcellular Location : Cytoplasm. Cytoplasm, perinuclear region. Cell projection, growth cone. Membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Cell projection, axon. Golgi apparatus. Endosome. Note=Associated with punctate structures in the perinuclear cytoplasm, axons, and growth cones of developing neurons. SCG10 exists in both soluble and membrane-bound forms.

Tissue Specificity : Neuron specific.

Post-translational modifications : Sumoylated.

Phosphorylated mostly by MAPK8, but also by MAPK9 and MAPK10 in the developing brain cortex.

N-terminal palmitoylation promotes specific anchoring to the cytosolic leaflet of Golgi membranes and subsequent vesicular trafficking along dendrites and axons. Neuronal Stathmins are substrates for palmitoyltransferases ZDHHC3, ZDHHC7 and ZDHHC15.

Similarity : Belongs to the stathmin family.

Database links : UniProtKB/Swiss-Prot: Q93045.3

STMN2神經(jīng)生長相關(guān)蛋白又稱：頸上神經(jīng)節(jié)神經(jīng)特異性因子10，該蛋白可調(diào)節(jié)神經(jīng)細(xì)胞的分化、神經(jīng)細(xì)胞軸突的增殖及細(xì)胞骨架的構(gòu)成。

抗體的鑒定：

1）磷酸化神經(jīng)生長相關(guān)蛋白SCG10抗體說明書 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力。抗體的特異性高,它的識別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗(yàn)測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。

Anti-MSLN /FITC 熒光素標(biāo)記間皮素抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Phospho-TNK1 (Tyr277)/FITC 熒光素標(biāo)記磷酸化非受體型酪氨酸蛋白激酶Tnk1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

5-羥色胺受體1A抗體 Anti-5-HTR1A 0.1ml

Mouse Anti-Rabbit IgM/Cy5 Cy5標(biāo)記的小鼠抗兔IgM 0.1ml

GLIPR2 英文名稱： 腦相關(guān)蛋白2抗體 0.2ml

Rhesus antibody Rh PSD93 突觸后密度蛋白93抗體 規(guī)格 0.2ml

Anti-Phospho-TNK1 (Tyr277)/FITC 熒光素標(biāo)記磷酸化非受體型酪氨酸蛋白激酶Tnk1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-EGF-R/FITC 熒光素標(biāo)記表皮生長因子受體抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Phospho-MYPT1 (Thr853) /FITC 熒光素標(biāo)記磷酸化肌球蛋酸酶抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh C2orf89 2號染色體開放閱讀框89抗體 規(guī)格 0.2ml

CCR-6/CD196 peptide 細(xì)胞表面趨化因子受體6抗原 0.5mg

HRH4/GPCR105 英文名稱： 組織胺H4受體抗體 0.1ml

Rhesus antibody Rh SAP62 剪接體相關(guān)蛋白62抗體 規(guī)格 0.2ml

Anti-Phospho-MYPT1 (Thr853) /FITC 熒光素標(biāo)記磷酸化肌球蛋酸酶抗體IgGMulti-class antibodies規(guī)格： 0.2ml

D1R(Human Dopamine D1 receptor) ELISA Kit 人多巴胺D1受體Multi-class antibodies規(guī)格： 48T

Anti-LATS1 抑制基因LATS1抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh KT3 tag KT3 tag抗體 規(guī)格 0.1ml

irGH(Human Immunoreactive growth hormone) ELISA Kit 人免疫反應(yīng)性生長激素 96T

Phospho-PIM1(Tyr218) 英文名稱： 磷酸化前病毒整合位點(diǎn)蛋白1抗體 0.1ml

CLSTN1 英文名稱： 相關(guān)類鈣粘蛋白CS1抗體 0.1ml

Anti-LATS1 抑制基因LATS1抗體Multi-class antibodies規(guī)格： 0.2ml

CD14 Others Cynomolgus 食蟹猴 CD14 人細(xì)胞裂解液 (陽性對照)

CM-R089大鼠皮膚肥大細(xì)胞完全培養(yǎng)基100mL

小鼠巨噬細(xì)胞MMa

22RV1細(xì)胞，人細(xì)胞 豬腎細(xì)胞,LLC-PK1細(xì)胞 人腎皮質(zhì)上皮細(xì)胞HRCEpiC

HPAC(人*腺泡上皮癌) 5×106cells/瓶×2

293T(人胚腎細(xì)胞) 5×106cells/瓶×2 CM-H051人胎盤滋養(yǎng)層細(xì)胞完全培養(yǎng)基100mL 人膀胱基質(zhì)成纖維細(xì)胞RNAHBdSF miRNA5 μg

CL-0346HBE(人上皮樣細(xì)胞)5×106cells/瓶×2

DMT1 Others Human 人 DNMT2 / DMT1 桿狀病毒-昆蟲細(xì)胞裂解液 (陽性對照)

人肺泡上皮細(xì)胞總RNAHPAEpiC NA

K562細(xì)胞，慢性髓原 人胚肺二倍體細(xì)胞,HLF-02細(xì)胞 lovo, 人細(xì)胞

豹貓肺成纖維樣細(xì)胞;LCL1

HA Others H5N1 甲型 H5N1 (A/Anhui/1/2005) 血凝素 (Hemagglutinin / HA) 人細(xì)胞裂解液 (陽性對照)

磷酸化神經(jīng)生長相關(guān)蛋白SCG10抗體說明書 CL-0345HB611(人細(xì)胞)5×106cells/瓶×2

MFI2 Others Mouse 小鼠 MFI2 / CD228 / melanoansferrin 人細(xì)胞裂解液 (陽性對照)

小鼠肺大動脈平滑肌細(xì)胞完全培養(yǎng)基 100mL

C3H/10T1/2, Clone 8小鼠胚胎成纖維細(xì)胞 C3H/10T1/2, Clone 8 mouse embryonic fibroblast MEM培養(yǎng)基(GIBCO)+10%FBS

IL1B Protein Cynomolgus 重組食蟹猴 IL-1 beta / IL1B 蛋白

NCI-H520(人肺鱗癌細(xì)胞) 5×106cells/瓶×2 T/G HA-VSMC(人主動脈平滑肌細(xì)胞)