產(chǎn)品訂購信息：
英文名稱  Anti-Ube2H/UBCH2 

中文名稱   泛素激活酶2H抗體規(guī)格 

別    名  E2 20K; Ubc H2; UBC8; UBCH 2; UBCH; UBCH2; Ube2h; UBE2H_HUMAN; Ubiquitin carrier protein H; Ubiquitin conjugating enzyme E2 H; Ubiquitin conjugating enzyme E2H (UBC8 homolog yeast); Ubiquitin conjugating enzyme E2H; Ubiquitin protein ligase H; Ubiquitin-conjugating enzyme E2 H; Ubiquitin-conjugating enzyme E2-20K; Ubiquitin-protein ligase H.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Mouse, Rat, Chicken, Dog, Cow, Rabbit 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 免疫學(xué) 表觀遺傳學(xué)

蛋白分子量  predicted molecular weight: 21kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human Ube2H/UBCH2 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

泛素激活酶2H抗體規(guī)格 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 UBE2H, also known as UBC8, UBCH, UBCH2 or E2-20K, is a 183 amino acid protein involved in ubiquitin-mediated protein degradation. Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). One of several members of the ubiquitin-conjugating enzyme family, UBE2H functions as an E2 ubiquitin-conjugating enzyme that acts to catalyze the covalent attachment of ubiquitin residues to various proteins, including Histone H2A. UBE2H shares 100% identity with its mouse counterpart and 98% identity with its frog and zebrafish homologs, suggesting a conserved function between species. Multiple isoforms of UBE2H exist due to alternative splicing events.

Function : Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A.

Similarity : Belongs to the ubiquitin-conjugating enzyme family.

Database links : UniProtKB/Swiss-Prot: P62256.1

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Anti-Phospho-TLK1 (Ser695) 磷酸化調(diào)節(jié)染色體組裝激酶1抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh GLUT8 葡萄糖轉(zhuǎn)運蛋白8抗體 規(guī)格 0.2ml

CCSA-3(Human colon cancer-specific antigen-3) ELISA kit 人專一抗原3 96T

NKp80 英文名稱： 細(xì)胞毒性受體NK-p80抗體 0.2ml

ANTXR2 英文名稱： 炭疽毒素受體2抗體 0.2ml

Anti-Phospho-TLK1 (Ser695) 磷酸化調(diào)節(jié)染色體組裝激酶1抗體Multi-class antibodies規(guī)格： 0.1ml

Anti-phospho-GAB2 (Tyr452) /FITC 熒光素標(biāo)記磷酸化接頭蛋白Gab 2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Phospho-eNOS (Thr495)/FITC 熒光素標(biāo)記磷酸化合成酶3抗體(內(nèi)皮型)IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh BrdU 溴脫氧尿苷抗體 規(guī)格 0.2ml

AQP1(Aquaporin1) 水通道蛋白-1抗原 0.5mg

HS1BP3 英文名稱： 造血干細(xì)胞特異性相關(guān)結(jié)合蛋白3抗體 0.2ml

Rhesus antibody Rh RGS5 G蛋白信號轉(zhuǎn)導(dǎo)調(diào)節(jié)因子5抗體 規(guī)格 0.2ml

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26S PSM(Human 26S proteasome) ELISA Kit 人26S蛋白酶體Multi-class antibodies規(guī)格： 48T

Anti-NPM 核仁磷酸蛋白抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh IGSF5 免疫球蛋白超家族成員5抗體 規(guī)格 0.2ml

TRH(Mouse thyrotropin-releasing hormone) ELISA Kit 小鼠促甲狀腺素釋放激素 96T

phospho-PIK3R1(Tyr467) 英文名稱： 磷酸化磷脂酰肌醇激酶抗體 0.1ml

Alpha s1 Casein 英文名稱： α-s1酪蛋白抗體 0.2ml

Anti-NPM 核仁磷酸蛋白抗體Multi-class antibodies規(guī)格： 0.2ml

DPYS Others Human 人 DPYS / Dihydropyrimidinase 桿狀病毒-昆蟲細(xì)胞裂解液 (陽性對照)

CM-H127人晶狀體上皮細(xì)胞完全培養(yǎng)基100mL

人軟骨細(xì)胞HC-a

羅猴胎腎細(xì)胞;MA104 腦細(xì)胞，C6細(xì)胞 NCI-H446 [H446](小細(xì)胞細(xì)胞)

人細(xì)胞;DU 145 [DU145;DU-145]

TNFRSF14 Others Mouse 小鼠 HVEM / TNFRSF14 人細(xì)胞裂解液 (陽性對照)

CL-0339G-401(人Wilms細(xì)胞)5×106cells/瓶×2

PHKG1 Others Human 人 PHKG1 桿狀病毒-昆蟲細(xì)胞裂解液 (陽性對照)

人食道平滑肌細(xì)胞總RNAHESMC NA

RD細(xì)胞，惡性胚胎橫紋肌瘤 人細(xì)胞系,Tul-1細(xì)胞 原代軟骨細(xì)胞特制基礎(chǔ)無血清培養(yǎng)基Many types of cells包裝：500/100ml

豹貓肺成纖維樣細(xì)胞;LCL2

HA Others H5N1 甲型 H5N1 (A/Anhui/1/2005) 血凝素HA1 (Hemagglutinin) 人細(xì)胞裂解液 (陽性對照)

泛素激活酶2H抗體規(guī)格 CL-0338G1(發(fā)綠色熒光的小鼠胚胎干細(xì)胞)5×106cells/瓶×2

ESAM Others Mouse 小鼠 ESAM / Endothelial Cell Adhesion Molecule 人細(xì)胞裂解液 (陽性對照)

小鼠肺大動脈內(nèi)皮細(xì)胞完全培養(yǎng)基 100mL

Eca-109人細(xì)胞 Human esophageal cancer cell line Eca-109. 1640+10% FBS

IL1B Protein Rat 重組大鼠 IL-1 beta / IL1B 蛋白 (mature form)

NCI-H524(人非小細(xì)胞細(xì)胞) 5×106cells/瓶×2 HUVEC(人臍內(nèi)皮細(xì)胞)

抗體的生物素化標(biāo)記實驗要點：

1. 泛素激活酶2H抗體規(guī)格 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）泛素激活酶2H抗體規(guī)格 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。