產(chǎn)品訂購(gòu)信息：
英文名稱  Anti-UBE1/E1 Ubiquitin Activating Enzyme 

中文名稱   泛素激活酶E1抗體費(fèi)用 

別    名  A1S9; A1S9 protein; A1S9T and BN75 temperature sensitivity complementing; A1S9T; A1ST; GXP 1; GXP1; MGC4781; Protein A1S9; Uba1; UBA1_HUMAN; UBE 1 ; UBE 1X; UBE1; UBE1X; Ubiquitin activating enzyme E1; Ubiquitin-activating enzyme E1; Ubiquitin-like modifier-activating enzyme 1.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來(lái)源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 表觀遺傳學(xué)

蛋白分子量  predicted molecular weight: 118kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human UBE1 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

泛素激活酶E1抗體費(fèi)用 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008].

Function : Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.

Subunit : Monomer (By similarity). Interacts with GAN (via BTB domain).

Post-translational modifications : ISGylated.

DISEASE : Defects in UBA1 are the cause of spinal muscular atrophy X-linked type 2 (SMAX2) [MIM:301830]; also known as X-linked lethal infantile spinal muscular atrophy, distal X-linked arthrogryposis multiplex congenita or X-linked arthrogryposis type 1 (AMCX1). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX2 is a lethal infantile form presenting with hypotonia, areflexia, and multiple congenital contractures.

Similarity : Belongs to the ubiquitin-activating E1 family.

Database links : UniProtKB/Swiss-Prot: P22314.3

Anti-Lpin1 protein/FITC 熒光素標(biāo)記Lpin1 抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-phospho-Synapsin I (Ser9) /FITC 熒光素標(biāo)記磷酸化神經(jīng)突觸素1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Adiponectin Receptor 1 脂聯(lián)素受體1抗體 規(guī)格 0.2ml

Rabbit Anti-Goat IgG/Alexa Fluor 555 Alexa Fluor 555標(biāo)記的兔抗羊IgG 0.1ml

GPR48 英文名稱： G蛋白偶聯(lián)受體48抗體 0.1ml

Rhesus antibody Rh Rabbit Anti-chicken IgM/FITC FITC標(biāo)記的兔抗雞IgM 規(guī)格 0.3ml

Anti-phospho-Synapsin I (Ser9) /FITC 熒光素標(biāo)記磷酸化神經(jīng)突觸素1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-p-ERK1/p-MAPK-1/2 /FITC 熒光素標(biāo)記磷酸化原活化蛋白激酶1/2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-MTL/FITC 熒光素標(biāo)記胃動(dòng)素抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh C1orf172 1號(hào)染色體開放閱讀框172抗體 規(guī)格 0.2ml

TGF- Beta1 (Ttansforming Growth Factor – Beta1) 轉(zhuǎn)移生長(zhǎng)因子–β1(抗原) 0.5mg

HSP70 英文名稱： 熱休克蛋白70抗體 0.1ml

Rhesus antibody Rh RRAGA RAS相關(guān)GTP結(jié)合蛋白A抗體 規(guī)格 0.2ml

Anti-MTL/FITC 熒光素標(biāo)記胃動(dòng)素抗體IgGMulti-class antibodies規(guī)格： 0.2ml

EAR3(Human eosinophil-associated ribonuclease A family member 3)ELISA Kit 人嗜酸性相關(guān)之RNA水解酵素家族成員3Multi-class antibodies規(guī)格： 48T

Anti-MUC5AC/Mucin 5AC 胃粘液素抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh Insulin 抗體 規(guī)格 0.1ml

ACA-IgM(Human anti-cardiolipin antibody IgM) ELISA Kit 人抗心磷脂抗體IgM 96T

Phospho-PDGFRA(Tyr1018) 英文名稱： 磷酸化血小板源性生長(zhǎng)因子受體-α抗體 0.1ml

C1D 英文名稱： 核DNA結(jié)合蛋白C1D抗體 0.1ml

Anti-MUC5AC/Mucin 5AC 胃粘液素抗體Multi-class antibodies規(guī)格： 0.1ml

CD200 Others Cynomolgus 食蟹猴 CD200 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

平滑肌細(xì)胞Many types of cells包裝：5 × 105次方(1ml)

615小鼠滑膜瘤株;ZM755

LTEP-s細(xì)胞，人肺鱗癌細(xì)胞 小鼠皮膚細(xì)胞,JB6-C30細(xì)胞 CL-0114Hs 578T(人癌細(xì)胞)5×106cells/瓶×2

Tca-8113(人舌鱗癌細(xì)胞) 5×106cells/瓶×2

HBdMEC-c 人膀胱微內(nèi)皮細(xì)胞(HBdMEC) 500,000cells 皮下脂肪細(xì)胞Many types of cells包裝：5 × 105次方(1ml)

CL-0282HO-8910PM(人高轉(zhuǎn)移細(xì)胞)5×106cells/瓶×2

TNFSF11 Others Mouse 小鼠 RANKL / OPGL / TNFSF11 / CD254 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

小鼠頜下腺上皮細(xì)胞完全培養(yǎng)基 100mL

L W-3A小鼠皮下結(jié)締組織細(xì)胞 L W-3A mice subcutaneous connective tissue cells DMEM培養(yǎng)基+10%FBS

IL1A Protein Mouse 重組小鼠 IL-1 alpha / IL1A / IL1F1 蛋白

P3X63Ag8.653(小鼠細(xì)胞) 5×106cells/瓶×2 L6(大鼠成肌細(xì)胞)

泛素激活酶E1抗體費(fèi)用 CL-0278HCCLM3(人高轉(zhuǎn)移細(xì)胞)5×106cells/瓶×2

IL17RB Others Mouse 小鼠 IL17BR / IL17RB / IL-17 Receptor B 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

人晶狀體上皮細(xì)胞cDNAHLEpiC cDNA

LCC1細(xì)胞，人癌細(xì)胞 VERO衍生株,SVP細(xì)胞 成骨細(xì)胞Many types of cells包裝：5 × 105次方(1ml)

TG-905(人腦膠質(zhì)母細(xì)胞瘤細(xì)胞) 5×106cells/瓶×2

HPMEC Pellet 人肺微內(nèi)皮細(xì)胞團(tuán)塊 > 1 mio.cells 多聚賴氨酸 1 mg/mlPLL

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 泛素激活酶E1抗體費(fèi)用 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；

3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）泛素激活酶E1抗體費(fèi)用 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。