抗體的生物素化標(biāo)記實驗要點：

1. 尿皮質(zhì)素UCN3抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細胞的熒光標(biāo)記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導(dǎo)致高本底。

產(chǎn)品訂購信息：
英文名稱  Anti-Urocortin 3 

中文名稱   尿皮質(zhì)素UCN3抗體品牌 

別    名  SCP; SPC; Stresscopin; Ucn III; UCN3; UCN3 urocortin 3 stresscopin; UCNIII; Urocortin 3 stresscopin; Urocortin III; UCN3_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Pig, Cow, Horse, Rabbit 

產(chǎn)品類型   一抗  

研究領(lǐng)域     心 細胞生物 神經(jīng)生物學(xué) 生長因子和激素 病

蛋白分子量  predicted molecular weight: 4.6kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human UCN3 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

尿皮質(zhì)素UCN3抗體品牌 產(chǎn)品應(yīng)用    WB= ELISA= IHC-P= IHC-F= IF=

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Urocortin 3 is a member of the sauvagine/corticotropin-releasing factor/urotensin I family. It is structurally related to the corticotropin-releasing factor (CRF) gene and the encoded product is an endogenous ligand for CRF type 2 receptors. In the brain it may be responsible for the effects of stress on appetite. In spite of the gene family name similarity, the product of this gene has no sequence similarity to urotensin II.

A powerful cytoprotective effect of urocortin peptide administration against ischemia and reperfusion injury has been demonstrated in isolated cardiomyocyte models, as well as in the intact heart both in vitro and in vivo.

Function : Urocortin 3 is a member of the sauvagine/corticotropin-releasing factor/urotensin I family. It is structurally related to the corticotropin-releasing factor (CRF) gene and the encoded product is an endogenous ligand for CRF type 2 receptors. In the brain it may be responsible for the effects of stress on appetite. In spite of the gene family name similarity, the product of this gene has no sequence similarity to urotensin II. A powerful cytoprotective effect of urocortin peptide administration against ischemia and reperfusion injury has been demonstrated in isolated cardiomyocyte models, as well as in the intact heart both in vitro and in vivo.

Subunit : Binds with high affinity to CRF receptors 2-alpha and 2-beta.

Subcellular Location : Secreted

Similarity : Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family.

Database links : UniProtKB/Swiss-Prot: Q969E3.2

抗體的鑒定：

1）尿皮質(zhì)素UCN3抗體品牌 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力。抗體的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。

Anti-Phospho-HSL (Ser660)/FITC 熒光素標(biāo)記磷酸化荷爾蒙敏感脂肪酶抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-PIBF/FITC 熒光素標(biāo)記孕激素誘導(dǎo)阻斷因子抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh ASM/Acid sphingomyelinase 酸性神經(jīng)鞘磷脂酶抗體 規(guī)格 0.1ml

Donkey Anti-Guinea pig IgG/Cy3 Cy3標(biāo)記的驢抗豚鼠IgG 0.1ml

GLUT6 英文名稱： 葡萄糖轉(zhuǎn)運蛋白6抗體 0.2ml

Rhesus antibody Rh Rabbit Anti-mouse IgG/Gold 膠體金標(biāo)記的兔抗小鼠IgG 規(guī)格 0.5ml

Anti-PIBF/FITC 熒光素標(biāo)記孕激素誘導(dǎo)阻斷因子抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-GAPDH /FITC 熒光素標(biāo)記3-磷酸甘油醛脫氫酶抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-NPY1R/FITC 熒光素標(biāo)記神經(jīng)肽Y1受體抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh BPIL2 殺菌/通透性增加蛋白樣2抗體 規(guī)格 0.2ml

IGFBP-5 ( Insulin-like Growth Factor Binding Protein 5、IBP5) 樣生長因子結(jié)合蛋白-5(抗原) 0.5mg

HOXA7 英文名稱： 同源盒基因HOXA7蛋白抗體 0.1ml

Rhesus antibody Rh Ret/HSCR1 原癌基因酪氨酸蛋白激酶受體RET/多發(fā)性腺瘤和甲狀腺髓樣癌蛋白抗體 規(guī)格 0.1ml

Anti-NPY1R/FITC 熒光素標(biāo)記神經(jīng)肽Y1受體抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Human Free Protein S,FP-S ELISA Kit 人游離蛋白SMulti-class antibodies規(guī)格： 48T

Anti-Pan-ras Pan ras抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh H5N1 Matrix Protein 2 A型病毒H5N1-M2蛋白抗體 規(guī)格 1ml

TCC C5b-9(Mouse terminal complement complex C5b-9) ELISA Kit 小鼠末端補體復(fù)合物C5b-9 96T

phospho-PRKD1(Tyr463) 英文名稱： 磷酸化蛋白激酶C mu型抗體 0.1ml

BXDC1 英文名稱： BXDC1蛋白抗體 0.2ml

Anti-Pan-ras Pan ras抗體Multi-class antibodies規(guī)格： 0.1ml

EPHB2 Others Human 人 EphB2 / Hek5 (aa 570-987) 桿狀病毒-昆蟲細胞裂解液 (陽性對照)

人腦星形膠質(zhì)母細胞瘤;U-87 MG [U87MG;U87 MG]

mRTEC, 小鼠腎小管上皮細胞

RLF, 大鼠淋巴成纖維細胞 STJ.EPI.H7細胞 COS-1(SV40 轉(zhuǎn)化的非洲綠猴腎細胞)

人結(jié)直腸腺癌細胞;HCT 116 [HCT116]

REG1A Others Human 人 REG1A / PSPS 人細胞裂解液 (陽性對照)

CL-0438SF767(人細胞)5×106cells/瓶×2

NRG1 Others Canine 狗 NRG1-alpha Protein (ECD) 人細胞裂解液 (陽性對照)

人肺動脈成纖維細胞RNAHPAF miRNA5 μg

兔晶體上皮細胞永生系;N/N1003A(RLE) 細胞，HCT 116細胞 M-1細胞，小鼠腎集合管細胞(SV40轉(zhuǎn)化)

人胚肺細胞系;KuMA

HA Others H5N1 甲型 H5N1 (A/Cambodia/R0405050/2007) 血凝素 (Hemagglutinin / HA) 人細胞裂解液 (陽性對照)

尿皮質(zhì)素UCN3抗體品牌 CL-0436SF17(人細胞)5×106cells/瓶×2

CCNA1 Others Human 人 CCNA1 / Cyclin-A1 桿狀病毒-昆蟲細胞裂解液 (陽性對照)

人肺動脈內(nèi)皮細胞RNAHPAEC miRNA5 μg

H08910細胞，細胞 人細胞,H125細胞 MLF, 小鼠淋巴成纖維細胞

豚鼠皮膚細胞;GP-S1

TNFRSF17 Others Mouse 小鼠 TNFRSF17 人細胞裂解液 (陽性對照)