磷酸化自噬相關(guān)蛋白1抗體費(fèi)用 英文名稱(chēng)  Anti-Phospho-ATG1(Ser556)

中文名稱(chēng)  磷酸化自噬相關(guān)蛋白1抗體費(fèi)用 

別    名 ULK1 (phospho S556); ULK1 (phospho Ser556); p-ULK1 (Ser556); ATG 1; ULK1; ATG1; ATG1A; Serine/threonine protein kinase ULK1; Serine/threonine protein kinase Unc51.1; ULK 1; Unc 51 (C. elegans) like kinase 1; UNC 51; Unc 51 like kinase 1; Unc-51-like kinase 1; Unc-51 like kinase 1 (C. elegans); UNC51; ULK1_HUMAN; Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog; hATG1.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg

抗體來(lái)源  Rabbit

克隆類(lèi)型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Pig, Horse

產(chǎn)品類(lèi)型   一抗 磷酸化抗體  

研究領(lǐng)域    細(xì)胞生物 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 116kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human ULK1 around the phosphorylation site of Ser556

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化自噬相關(guān)蛋白1抗體費(fèi)用 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 ULK1 belongs to the serine/threonine protein kinase family. It is involved in axon growth and plays an essential role in neurite branching during sensory axon outgrowth. Knockdown of ULK1 results in impaired endocytosis of nerve growth factor (NGF), excessive axon arborization, and severely stunted axon elongation indicating that ULK1 mediates a non clathrin coated endocytosis in sensory growth cones. Knockdown of ULK1 also inhibits the autophagic response. It appears to act as a convergence point for multiple signals that regulate autophagy, and in turn interacts with a large number of autophagy related (Atg) proteins.

Function : Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation.

Subunit : Interacts with GABARAP and GABARAPL2. Interacts (via C-terminus) with ATG13/KIAA0652. Part of a complex consisting of ATG13/KIAA0652, ULK1 and RB1CC1. Associates with the mammalian target of rapamycin complex 1 (mTORC1) through an interaction with RPTOR; the association depends on nutrient conditions and is reduced during starvation.

Subcellular Location : Cytoplasm, cytosol. Preautophagosomal structure. Note=Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.

Tissue Specificity : Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.

Post-translational modifications : Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.

Similarity : Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.

Contains 1 protein kinase domain.

Database links : UniProtKB/Swiss-Prot: O75385.2

Atg1是一種絲氨酸/蘇氨酸蛋白激酶。Atg1的激脢活性是CVT信號(hào)傳導(dǎo)通路以及細(xì)胞自噬所必須的。Atg1可以和一些執(zhí)行細(xì)胞自噬的蛋白相互作用，而且有很多調(diào)控細(xì)胞自噬的信號(hào)傳導(dǎo)通路匯集在Atg1。因此Atg1可能是一個(gè)可以調(diào)控細(xì)胞自噬很多步驟的一個(gè)調(diào)節(jié)關(guān)鍵點(diǎn)。但在較高等的真核生物中，Atg1的角色仍然不是很清楚。通過(guò)目前的研究已經(jīng)比較了解細(xì)胞自噬可以導(dǎo)致細(xì)胞的死亡，但是如何導(dǎo)致死亡的分子機(jī)制還不清楚，有待于進(jìn)一步研究。

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