技術(shù)外包服務(wù):

★人乳頭狀瘤E6相關(guān)蛋白抗體費(fèi)用 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等

★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。

實(shí)驗(yàn)流程：

人乳頭狀瘤E6相關(guān)蛋白抗體費(fèi)用 英文名稱  Anti-UBE3A/E6-AP

中文名稱  人乳頭狀瘤E6相關(guān)蛋白抗體費(fèi)用 

別    名 ANCR; Angelman syndrome; AS; CTCL tumor antigen se37 2; E6 AP; E6AP antibody E6AP ubiquitin protein ligase; E6AP ubiquitin protein ligase; E6AP ubiquitin-protein ligase; EPVE6AP; HPVE6A; Human papilloma virus E6 associated protein Angelman syndrome; Human papilloma virus E6 associated protein; Human papillomavirus E6-associated protein; NY REN 54 antigen; NY REN 54 antigen; Oncogenic protein associated protein E6 AP; Oncogenic protein associated protein E6AP; Oncogenic protein-associated protein E6-AP; Renal carcinoma antigen NY REN 54; Renal carcinoma antigen NY-REN-54; UBE 3A; Ube3a; UBE3A protein; UBE3A_HUMAN; Ubiquitin protein ligase E3A; Ubiquitin-protein ligase E3A.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep

產(chǎn)品類型   一抗  

研究領(lǐng)域     細(xì)胞生物 染色質(zhì)和核信號(hào) 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞周期蛋白 表觀遺傳學(xué) 泛素

蛋白分子量  predicted molecular weight: 105kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human UBE3A/E6-AP

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

人乳頭狀瘤E6相關(guān)蛋白抗體費(fèi)用 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 E6-associating protein is a component of the ubiquitin-mediated proteolytic pathway, which selectively targets proteins for degradation by the 26S proteasome. Ubiquitin (Ub) is directly conjugated to protein substrates by the transfer of Ub from an E2 ubiquitin conjugating enzyme to the target protein. This conjugation is facilitated by the enzymatic activity of E3 ubiquitin ligase family members such as E6-AP. Several substrates of E6-AP have been identified and include the tumor suppressor protein p53 and the mammalian homolog of Rad23, HHR23A. Previous studies have indicated that E6-AP associates with the human papillomavirus E6 oncogene, which complexes with p53 and thereby potentiates E6-AP mediated ubiquitination of p53. Genetic mutations that impair E6-AP activity result in the accumulation of p53 in the cytoplasm, and, in many instances, these mutations are associated with the development of the rare neurodevelopmental disorder Angelman syndrome (AS), which is characterized by severe motor dysfunction and mental retardation.

Function : E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo.

Subcellular Location : Nucleus.

Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR.

DISEASE : Defects in UBE3A are a cause of Angelman syndrome (AS) [MIM:105830]; also known as 'happy puppet syndrome'. AS is characterized by features of severe motor and intellectual retardation, microcephaly, ataxia, frequent jerky limb movements and flapping of the arms and hands, hypotonia, hyperactivity, hypopigmentation, seizures, absence of speech, frequent smiling and episodes of paroxysmal laughter, and an unusual facies characterized by macrostomia, a large mandible and open-mouthed expression, a great propensity for protruding the tongue ('tongue thrusting'), and an occipital groove.

Similarity : Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.

Database links : UniProtKB/Swiss-Prot: Q05086.4

全、新、優(yōu)、品、好四大特點(diǎn)：

人乳頭狀瘤E6相關(guān)蛋白抗體費(fèi)用 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時(shí)沒有任何的后顧之憂。

正在熱銷的相關(guān)產(chǎn)品：

PAK7/PAK5/MBT 激活激酶PAK7/PAK5抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-ATF2 活化復(fù)制因子2抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh phospho-APP/ABPP (Ser730) 磷酸化APP淀粉樣肽前體蛋白抗體 規(guī)格 0.1ml

Somatostatin 生長(zhǎng)抑素 濃縮液 0.1ml 進(jìn)口分裝

UBE2D4 英文名稱： 泛素蛋白連接酶D4抗體 0.2ml

DDRGK1 英文名稱： DDRGK1蛋白抗體 0.2ml

Anti-ATF2 活化復(fù)制因子2抗體Multi-class antibodies規(guī)格： 0.1ml

MMP-7 ELISA kit 大鼠基質(zhì)金屬蛋白酶7Multi-class antibodies規(guī)格： 48T

Anti-ALT1/GPT 1/FITC 熒光素標(biāo)記谷丙氨酸氨基轉(zhuǎn)移酶1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh fatty acid elongase 脂肪酸延長(zhǎng)酶抗體 規(guī)格 1ml

gp210 ELISA Kit 大鼠抗核膜糖蛋白210抗體 96T

phospho-MAPK3(Tyr204) 英文名稱： 磷酸化原活化蛋白激酶3抗體 0.1ml

AAMP 英文名稱： 內(nèi)皮細(xì)胞遷移蛋白抗體 0.2ml

Anti-ALT1/GPT 1/FITC 熒光素標(biāo)記谷丙氨酸氨基轉(zhuǎn)移酶1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-VCAM-1/FITC(CD106) 熒光素標(biāo)記內(nèi)皮細(xì)胞粘附分子抗體IgGMulti-class antibodies規(guī)格： 0.2ml

CD33/Siglec-3(mouse ret) CD33抗原(大、小鼠)Multi-class antibodies規(guī)格： 0.5mg

細(xì)胞表面趨化因子受體7抗體 Anti-CCR-7 0.1ml

STK36 英文名稱： 絲酸/蘇酸激酶36融合同源蛋白抗體 0.2ml

ENC1 英文名稱： 外胚層神經(jīng)皮層蛋白1抗體 0.2ml

Rhesus antibody Rh Phospho-HER2(Tyr1248) 磷酸化HER2受體抗體 規(guī)格 0.1ml

CD33/Siglec-3(mouse ret) CD33抗原(大、小鼠)Multi-class antibodies規(guī)格： 0.5mg

大鼠可溶性髓系細(xì)胞觸發(fā)受體-1(sEM-1)ELISA試劑盒 ，英文名： sEM-1 ELISA Kit

人基質(zhì)金屬蛋白酶7(MMP-7)ELISA檢測(cè)試劑盒HumanMaixmetalloproteinase7,MMP-7ELISAkit 96T/48T

家蠶卵黃蛋白(LT)免疫試劑盒 Bombyx mori Livetin,LT ELISA Kit

CLIAKitforON(Humanosteonectin)ELISAKit人骨粘連蛋白規(guī)格：48T/96T

糖血清蛋白(GSP)化學(xué)比色法定量檢測(cè)試劑盒50次

ELISAKitγ-ECS人γ谷氨酰半胱氨酸合成酶規(guī)格：48T/96T

大鼠白細(xì)胞活化黏附因子(ALCAM)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

人卵清蛋白特異性IgG(OVA sIgG)免疫試劑盒 Human ovalbumin specific IgG,OVA sIgG ELISA Kit

Mouseβmannosidase,βManaseELISAKit小鼠β甘露糖苷酶(βManase)ELISA試劑盒規(guī)格：96T/48T

石蠟切片組織ERBETA蛋白表達(dá)NBT顯色光學(xué)顯微鏡檢測(cè)試劑盒10/20次

HumanProteinase3Aibody,PR3AbELISA試劑盒人蛋白酶3抗體(PR3Ab)ELISA試劑盒規(guī)格：96T/48T

HumaissuePolypeptideAigen，TPAELISAKit人組織多肽抗原(TPA)ELISA試劑盒規(guī)格：96T/48T

人乳頭狀瘤E6相關(guān)蛋白抗體費(fèi)用 大鼠簇集蛋白(CLU)ELISA試劑盒 ，英文名： CLU ELISA Kit

Rat leukoiene B4 (LTB4) ELISA Kit 大鼠白三烯B4(LTB4)ELISA試劑盒

RatEndostatin,ESELISAKit 大鼠內(nèi)皮抑素(ES)ELISA試劑盒 96T/48T 進(jìn)口分裝

CLIAKitforCTGFELISAKit大鼠結(jié)締組織生長(zhǎng)因子規(guī)格：48T/96T

細(xì)胞糖原磷酸化酶a(GLYCOGENPHOSPHORYLASE)活性酶連續(xù)循環(huán)比色法定量檢測(cè)試劑盒10次

ratEndothelin1,ET-1ELISAKit大鼠內(nèi)皮素1(ET-1)ELISA試劑盒規(guī)格：96T/48T