中文名稱  泛素硫酯酶L1+3抗體說明書 

英文名稱  Anti-UCHL1+3

別    名 Ubiquitin C terminal esterase L1; Ubiquitin carboxyl terminal esterase L1; Ubiquitin carboxyl terminal esterase L3; Ubiquitin carboxyl terminal hydrolase isozyme L1; Ubiquitin carboxyl terminal hydrolase isozyme L3; Ubiquitin thiolesterase; UCHL1; UCHL3.

產(chǎn)品屬性：

泛素硫酯酶L1+3抗體說明書 濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Horse, Rabbit, Sheep

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 泛素 Alzheimer's

蛋白分子量  predicted molecular weight: 25kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human UCHL1+3 (141-223aa)

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

泛素硫酯酶L1+3抗體說明書 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The two ubiquitin C-terminal hydrolase (UCH) enzymes, UCHL1 and UCHL3, deubiquitinate ubiquitin-protein conjugates and control the cellular balance of ubiquitin. UCHL1 and UCHL3 are both small proteins of ~220 amino acids that share more than 40% amino acid sequence identity. UCHL3 is universally expressed in all tissues, while UCHL1 is expressed exclusively in neuronal tissue, testis and ovary. The activity of UCHL3 is more than 200 fold higher than UCHL1 when a fluorogenic ubiquitin substrate is used. UCHL1 associates with monoubiquitin and UCHL3 binds to Nedd8, ubiquitin-like protein. UCHL1 and UCHL3 play a role in the regulation of neuronal development and spermatogenesis. UCHL1 is involved in the pathogenesis of Parkinson’s disease (PD) and Alzheimer’s disease (AD).

Function : Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

Subunit : Monomer. Homodimer. Interacts with SNCA (By similarity). Interacts with COPS5.

Subcellular Location : Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note=About 30% of total UCHL1 is associated with membranes in brain.

Tissue Specificity : Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.

Post-translational modifications : O-glycosylated

DISEASE : Defects in UCHL1 are the cause of Parkinson disease type 5 (PARK5) [MIM:613643]; also known as Parkinson disease autosomal dominant 5. PARK5 is a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (resting tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia.

Similarity : Belongs to the peptidase C12 family.

Database links : UniProtKB/Swiss-Prot: P09936.2

實驗流程：

全、新、優(yōu)、品、好四大特點：

泛素硫酯酶L1+3抗體說明書 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊，產(chǎn)品一旦售出，實驗過程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時沒有任何的后顧之憂。

技術(shù)外包服務(wù):

★泛素硫酯酶L1+3抗體說明書 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測序、載體構(gòu)建等

★蛋白工程：原核、哺乳動物蛋白表達(dá)系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。

正在熱銷的相關(guān)產(chǎn)品：

Phospho-PAK4 (Ser99) peptide 磷酸化p21激活激酶4多肽抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-ATF1 活化轉(zhuǎn)錄因子1抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh phospho-APP/ABPP (Thr743) 磷酸化APP淀粉樣肽前體蛋白抗體 規(guī)格 0.1ml

Stat1 濃縮液 0.1ml 進(jìn)口分裝

UBE2D3 英文名稱： 泛素蛋白連接酶D3抗體 0.2ml

DENND2A 英文名稱： DENND2A蛋白抗體 0.2ml

Anti-ATF1 活化轉(zhuǎn)錄因子1抗體Multi-class antibodies規(guī)格： 0.2ml

MAGE-1 Anti-gen (Melanoma Anti-gen Recognized by T-cells 1) 相關(guān)抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-AMACR α-甲基?；o酶A消旋酶抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Phospho-BRCA1(Ser1524) 磷酸化癌易感基因1抗體 規(guī)格 0.1ml

品名 規(guī)格 備注

phospho-VEGFR2 (Tyr1175) 英文名稱： 磷酸化內(nèi)皮生長因子受體2抗體 0.1ml

DCK 英文名稱： 脫氧胞苷激酶抗體 0.2ml

Anti-AMACR α-甲基?；o酶A消旋酶抗體Multi-class antibodies規(guī)格： 0.2ml

Anti-p-VEGFR2/FITC 熒光素標(biāo)記兔抗人、大、小鼠磷酸化內(nèi)皮生長因子受體2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Cdkn1c/p57/Kip2 (Cyclindependent kinase inhibitor 1C) 周期蛋白依賴激酶抑制因子1C抗原Multi-class antibodies規(guī)格： 0.5mg

細(xì)胞間粘附分子-3抗體 Anti-CD50/ICAM-3 0.1ml

Synphilin-1 英文名稱： 核突觸蛋白相互作用蛋白1抗體 0.1ml

Endo G 英文名稱： 核酸內(nèi)切酶G抗體 0.1ml

Rhesus antibody Rh phospho-GIT1(Tyr510) 磷酸化G蛋白偶聯(lián)受體激酶相互作用蛋白1 規(guī)格 0.1ml

Cdkn1c/p57/Kip2 (Cyclindependent kinase inhibitor 1C) 周期蛋白依賴激酶抑制因子1C抗原Multi-class antibodies規(guī)格： 0.5mg

大鼠可溶性髓系細(xì)胞觸發(fā)受體-2(sEM-2)ELISA試劑盒 ，英文名： sEM-2 ELISA Kit

人基質(zhì)金屬蛋白酶3(MMP-3)ELISA檢測試劑盒HumanMaixmetalloproteinase3,MMP-3ELISAkit 96T/48T

獼猴骨特異性堿性磷酸酶B(ALP-B)免疫試劑盒 Rhesus macaque bone-specific alkphase B,ALP-B ELISA Kit

CLIAKitforON(Mouseosteonectin)ELISAKit小鼠骨粘連蛋白規(guī)格：48T/96T

糖類總量-比色法定量檢測試劑盒20次

ELISAKitGGT人γ谷氨酰轉(zhuǎn)移酶規(guī)格：48T/96T

大鼠白細(xì)胞活化黏附因子(ALCAM)ELISA試劑盒 ，英文名： ALCAM ELISA Kit

Mouse beta naphthol (beta -naphthol) ELISA Kit 小鼠β萘酚(β-naphthol)ELISA試劑盒

ELISA 小鼠半乳糖凝集素-9(mouse Galectin-9) 48T/96T 進(jìn)口分裝

CLIAKitforirGH(HumanImmunoreactivegrowthhormone)ELISAKit人免疫反應(yīng)性生長激素規(guī)格：48T/96T

通用型番茄斑萎病毒(TomatoSpottedWiltVirus;TSWV)試劑盒20次

ELISAKitNAP-2/CXCL7大鼠中性粒細(xì)胞趨化蛋白2規(guī)格：48T/96T

泛素硫酯酶L1+3抗體說明書 大鼠催產(chǎn)素(OT)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

人膜結(jié)合型IgM(mIgM)免疫試劑盒 Human Membrane IgM,mIgM ELISA kit

MouseTissuefactorpathwayinhibitor,TFPIELISAKit小鼠組織因子途徑抑制物(TFPI)ELISA試劑盒規(guī)格：96T/48T

肌酸激酶(CK)活性終點比色法定量檢測試劑盒20次

HumanPregnancySpecificβ1Glycoprotein,SP1/PSβ1-GELISA試劑盒人特異性β1糖蛋白(SP1/PSβ1-G)ELISA試劑盒規(guī)格：96T/48T

Humaestoteronerecepter，ELISAKit人睪同受體()ELISA試劑盒規(guī)格：96T/48T