抗體的生物素化標記實驗要點：

1. 泛素硫酯酶L3抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導(dǎo)致高本底。

產(chǎn)品訂購信息：
英文名稱  Anti-UCHL3 

中文名稱   泛素硫酯酶L3抗體品牌 

別    名  Ubiquitin carboxyl-terminal hydrolase isozyme L3; Ubiquitin thioesterase L3; Ubiquitin thiolesterase; Ubiquitin thiolesterase L3; UCH L3; UCH-L3; UCHL3; UCHL3_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 表觀遺傳學(xué) 泛素 Alzheimer's

蛋白分子量  predicted molecular weight: 26kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human UCHL3 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

泛素硫酯酶L3抗體品牌 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 UCH-L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCH-L1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. UCH-L1 is expressed in brain neurons. Examination of specific brain regions reveals expression in all areas tested, particularly in the substantia nigra. UCH-L1 represents 1 to 2% of total soluble brain protein. Its occurrence in Lewy bodies and its function in the proteasome pathway make it a compelling candidate gene in Parkinson disease. The gene which encodes UCH-L1 maps to human chromosome 4p14. The 230 amino acid human UCH-L3 protein is 54% identical to that of UCH-L1. UCH-L3 is the predominant thiol protease and has high-affinity binding sites for ubiquitin.

Function : Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3". Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory.

Subunit : Preferentially binds diubiquitin; the interaction does not hydrolyze diubiquitin but, in vitro, inhibits the hydrolyzing activity on other substrates.

Subcellular Location : Cytoplasm.

Tissue Specificity : Highly expressed in heart, skeletal muscle, and testis.

Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR.

Similarity : Belongs to the peptidase C12 family.

Database links : UniProtKB/Swiss-Prot: P15374.1

抗體的鑒定：

1）泛素硫酯酶L3抗體品牌 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。

P73 protein P73抑制基因抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-Angiotensin I 緊張素I抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh phospho-APP/ABPP(Tyr757) 磷酸化APP(Tyr757)淀粉樣肽前體蛋白抗體 規(guī)格 0.1ml

Surfacant Protein A(100-200) 濃縮液 0.1ml 進口分裝

UBE2D2 英文名稱： 泛素蛋白連接酶D2抗體 0.2ml

DHRS9 英文名稱： 短鏈脫氫酶/還原酶家族9抗體 0.1ml

Anti-Angiotensin I 緊張素I抗體Multi-class antibodies規(guī)格： 0.2ml

MMP-2/Gelatinase A ELISA Kit 大鼠基質(zhì)金屬蛋白酶2/明膠酶AMulti-class antibodies規(guī)格： 48T

Anti-AMACR/P504S /FITC 熒光素標記α-甲基?；o酶A消旋酶抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh FAT/CDHF7/FAT1 鈣粘蛋白家族成員7抗體 規(guī)格 0.1ml

IL-2R(Mouse Interleukin-2 receptor) ELISA kit 小鼠白介素2受體 96T

phospho-MAP3K7IP1(Thr431) 英文名稱： 磷酸化轉(zhuǎn)化生長因子β活化激酶結(jié)合蛋白1抗體 0.1ml

AADACL2 英文名稱： 芳香乙酰胺脫乙?；笜拥鞍?抗體 0.2ml

Anti-AMACR/P504S /FITC 熒光素標記α-甲基酰基輔酶A消旋酶抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Shrimp tropomyosin /FITC 熒光素標記南美白對蝦過敏原蛋白(蝦原肌球蛋白)抗體IgGMulti-class antibodies規(guī)格： 0.2ml

BACE( ASP2 ) β分泌酶(抗原)Multi-class antibodies規(guī)格： 0.5mg

孤菲肽受體/痛敏肽受體抗體 Anti-Nociceptin receptor 0.1ml

SNAP25 英文名稱： 突觸相關(guān)蛋白25抗體 0.1ml

Endomucin 英文名稱： 內(nèi)皮粘蛋白EMCN抗體 0.2ml

Rhesus antibody Rh Phospho-p53BP1 (Ser1778) 磷酸化p53結(jié)合蛋白1抗體 規(guī)格 0.1ml

BACE( ASP2 ) β分泌酶(抗原)Multi-class antibodies規(guī)格： 0.5mg

大鼠可溶性糖蛋白VI(sGPVI)ELISA試劑盒 ，英文名： sGPVI ELISA Kit

人內(nèi)皮細胞生長因子受體3(VEGFR-3)ELISA檢測試劑盒HumanVascuoarendothelialcellgrowthfactorreceptor3,VEGFR-3ELISAKit 96T/48T

鳥H5N1 IgG抗體ELSIA 試劑盒 Bird influenza A virus subtype H5N1 IgG aibody ELISA Kit

CLIAKitforOPG(RabbitOsteoprotegerin)ELISAKit兔骨保護素規(guī)格：48T/96T

糖類總量氨基熒光定量檢測試劑盒20次

ELISAKitPγ人γ肽規(guī)格：48T/96T

大鼠白細胞激活黏附因子(ALCAM)ELISA試劑盒 ，英文名： ALCAM ELISA Kit

Human beta 2 glycoprotein 1 aibody IgG (beta 2-GP1 Ab IgG) ELISA Kit 人β2糖蛋白1抗體IgG(β2-GP1 Ab IgG)ELISA試劑盒

AflatoxinELISAKit 霉毒素(Aflatoxin)ELISA試劑盒 96T/48T 進口分裝

CLIAKitforColI(HumanCollageypeI)ELISAKit人Ⅰ型膠原規(guī)格：48T/96T

細胞游離氨基酸含量比色法定量檢測試劑盒20次

Ratcholesterolesteransferprotein,CETPELISAKit大鼠膽固醇酯轉(zhuǎn)移蛋白(CETP)ELISA試劑盒規(guī)格：96T/48T

泛素硫酯酶L3抗體品牌 大鼠催產(chǎn)素(OT)ELISA試劑盒 ，英文名： OT ELISA Kit

The swine major histocompatibility complex class I (MHC I and /SLA I) ELISA Kit 豬主要組織相容性復(fù)合體Ⅰ類(MHCⅠ/SLAⅠ)ELISA試劑盒

ELISA 小鼠載脂蛋白E(mouse APO E) 48T/96T 進口分裝

CLIAKitforLMWH(Humanlowmolecularweightheparin)ELISAKit人低分子肝素規(guī)格：48T/96T

通用型DNA損傷彗星檢測試劑盒20次

ELISAKitα-glucosidase雞α葡萄糖苷酶規(guī)格：48T/96T