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泛素激活酶2（泛素激活酶E1相關(guān)蛋白抗體）說明書 英文名稱  Anti-Ube1L/UBE2

中文名稱  泛素激活酶2（泛素激活酶E1相關(guān)蛋白抗體）說明書 

別    名 D8; UBA7; Ube 1L; UBE 2; UBE2; Ubiquitin activating enzyme 2; Ubiquitin activating enzyme E1 homolog; Ubiquitin Activating Enzyme E1 Like; Ubiquitin activating enzyme E1 related protein; UBA7_HUMAN.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Pig, Cow, Horse, Rabbit

產(chǎn)品類型   一抗  

研究領(lǐng)域    細胞生物 免疫學 信號轉(zhuǎn)導 細胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子

蛋白分子量  predicted molecular weight: 112kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human Ube1L

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

泛素激活酶2（泛素激活酶E1相關(guān)蛋白抗體）說明書 產(chǎn)品應(yīng)用   IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein. [provided by RefSeq, Jul 2008].

Function : Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.

Subunit : Monomer (By similarity). Binds and is involved in the conjugation of G1P2/ISG15.

Tissue Specificity : Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.

Similarity : Belongs to the ubiquitin-activating E1 family.

Database links : UniProtKB/Swiss-Prot: P41226.2

實驗流程：

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