抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 神經(jīng)細(xì)胞胞漿蛋白9.5抗體（蛋白基因產(chǎn)物9.5）規(guī)格 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時,或位于酶的催化位點(diǎn)時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。

產(chǎn)品訂購信息：
英文名稱  Anti-UCHL1/PGP9.5 

中文名稱   神經(jīng)細(xì)胞胞漿蛋白9.5抗體（蛋白基因產(chǎn)物9.5）規(guī)格 

別    名  Gracile axonal dystrophy; Neuron cytoplasmic protein 9.5; Park 5; Park5; Parkinson Disease 5; PGP 9.5; PGP9.5; PGP95; Protein gene product 9.5; Ubiquitin C terminal esterase L1; Ubiquitin C terminal hydrolase (neuron specific); Ubiquitin C terminal hydrolase; Ubiquitin carboxyl terminal esterase L1; Ubiquitin carboxyl terminal hydrolase isozyme L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1; Ubiquitin thioesterase L1; Ubiquitin thiolesterase; Ubiquitin thiolesterase L1; UCH L1; UCH-L1; UCHL1; UCHL1_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Pig, Cow, Horse, Guinea Pig, G 

產(chǎn)品類型   一抗  

研究領(lǐng)域     細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué)

蛋白分子量  predicted molecular weight: 25kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PGP95 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

神經(jīng)細(xì)胞胞漿蛋白9.5抗體（蛋白基因產(chǎn)物9.5）規(guī)格 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 catalyzes the hydrolysis of ubiquitin carboxy-terminal thiolesters to form ubiquitin and a thiol; may play a role in neuropathic pain [RGD].

Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.

Function : Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

Subcellular Location : Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.

Tissue Specificity : Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.

Post-translational modifications : O-glycosylated.

DISEASE : Defects in UCHL1 are the cause of Parkinson disease type 5 (PARK5) [MIM:613643]; also known as Parkinson disease autosomal dominant 5. PARK5 is a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (resting tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia.

Similarity : Belongs to the peptidase C12 family.

該抗原是一種神經(jīng)特異性蛋白，廣泛分布于中樞與外周的神經(jīng)元和神經(jīng)纖維、神經(jīng)細(xì)胞、腎小管段、睪丸精原細(xì)胞、Leydig細(xì)胞、卵細(xì)胞以及與非黃體內(nèi)的某些細(xì)胞。用于標(biāo)記神經(jīng)元，對研究幾種人慢性神經(jīng)變性性疾病中廣泛存在的細(xì)胞包涵體特征較有意義。

抗體的鑒定：

1）神經(jīng)細(xì)胞胞漿蛋白9.5抗體（蛋白基因產(chǎn)物9.5）規(guī)格 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗(yàn)測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。

CAE-Ab Human 抗人抗體Multi-class antibodies

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SP Kit(Mouse) SP免疫組化檢測試劑盒(小鼠) 3ml

TXNDC9 英文名稱： 胚胎干細(xì)胞相關(guān)蛋白TXNDC9抗體 0.2ml

C8orf72 英文名稱： 8號染色體開放閱讀框72抗體 0.2ml

Anti-CLEC1/CLEC1A C型凝集素結(jié)構(gòu)域家族1成員A抗體Multi-class antibodies規(guī)格： 0.2ml

MIP2 (myocardial ischemic preconditioning upregulated protein 2) 巨噬細(xì)胞蛋白2抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-Angiopoietin 1 生成素-1抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh phospho-Beta-catenin(Tyr86) 磷酸化β 連環(huán)素蛋白抗體 規(guī)格 0.1ml

包被緩沖液(PH9.6) 100ml 進(jìn)口分裝

VTG 英文名稱： 魚、青鳉魚卵黃蛋白原抗體 0.2ml

DLGAP4 英文名稱： PSD95結(jié)合蛋白4抗體 0.2ml

Anti-Angiopoietin 1 生成素-1抗體Multi-class antibodies規(guī)格： 0.1ml

Anti-SSTR1/FITC 熒光素標(biāo)記生長抑素受體1(SSTR1)抗體IgGMulti-class antibodies規(guī)格： 0.2ml

AMPK Beta1 (AMP-activated Protein Kinase beta-1) 腺苷單磷酸活化蛋白激酶β1抗原Multi-class antibodies規(guī)格： 0.5mg

胰十二指腸同源異型盒基因抗體 anti-Pdx1 0.1ml

Serum Amyloid P 英文名稱： 血清淀粉樣蛋白P成份抗體 0.1ml

ENPP3 英文名稱： ENPP3抗體IgG 0.1ml

Rhesus antibody Rh phospho-Na+/K+-ATPase Alpha1(Ser 943) 磷酸化ATP酶α1多肽抗體 規(guī)格 0.2ml

AMPK Beta1 (AMP-activated Protein Kinase beta-1) 腺苷單磷酸活化蛋白激酶β1抗原Multi-class antibodies規(guī)格： 0.5mg

大鼠酪酸蛋白激酶JAK3(Jak3)ELISA試劑盒 ，英文名： Jak3 ELISA Kit

人壞死因子相關(guān)凋亡誘導(dǎo)配體3(AIL-R3)ELISA檢測試劑盒Humaumornecrosisfactor-relatedapoptosis-inducingligand3,AIL-R3ELISAKit 96T/48T

原激活的蛋白激酶/MAP激酶(MAPK)免疫試劑盒 Human mitogen activited protein kinase,MAPK ELISA Kit

CLIAKitforOrexinAELISAKit大鼠阿立新A規(guī)格：48T/96T

糖果糖精(saccharin)含量比色法定量檢測試劑盒20次

ELISAKitCEF人癌胚鐵蛋白規(guī)格：48T/96T

大鼠抑制因子受體(LIFR)ELISA試劑盒 ，英文名： LIFR ELISA Kit

Mouse beta endorphin (beta -EP) ELISA Kit 小鼠β內(nèi)啡肽(β-EP)ELISA試劑盒

ELISA 小鼠凝血因子X(mouse FX) 48T/96T 進(jìn)口分裝

CLIAKitforiso-Hyd(HumanIsoprenalineHydrochloride)ELISAKit人異規(guī)格：48T/96T

通用型丁酰膽堿酯酶活性熒光定量檢測試劑盒20次

ELISAKitTNFsR-Ⅰ大鼠壞死因子可溶性受體Ⅰ規(guī)格：48T/96T

神經(jīng)細(xì)胞胞漿蛋白9.5抗體（蛋白基因產(chǎn)物9.5）規(guī)格 大鼠單核細(xì)胞趨化蛋白3(MCP-3/CCL7)ELISA試劑盒 ，英文名： MCP-3/CCL7 ELISA Kit

Porcine acetylcholine (ACh) ELISA Kit 豬乙酰膽堿(ACh)ELISA試劑盒

ELISA 小鼠生成素-2(mouse Angiopoietin-2) 48T/96T 進(jìn)口分裝

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