中文名稱  質(zhì)子梯度調(diào)節(jié)蛋白1抗體說明書 

英文名稱  Anti-UQCRFS1

別    名 Complex III subunit 5; Cytochrome b6f complex iron sulfur subunit, chloroplastic; petC; PGR1; Plastohydroquinone:plastocyanin oxidoreductase iron sulfur protein; Proton gradient regulation protein 1; Rieske iron sulfur protein; RIP1; RIS1; RISP; Ubiquinol cytochrome C reductase rieske iron sulphur; UQCR5; Cytochrome b-c1 complex subunit Rieske, mitochondrial.

產(chǎn)品屬性：

質(zhì)子梯度調(diào)節(jié)蛋白1抗體說明書 濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit

產(chǎn)品類型   一抗  

研究領(lǐng)域     細胞生物 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子

蛋白分子量  predicted molecular weight: 22kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human UQCRFS1(131-180aa)

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

質(zhì)子梯度調(diào)節(jié)蛋白1抗體說明書 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 UQCRFS1 is an iron-sulfur Rieske protein belongs to major subunits of cytochrome b6f complex of photosynthetic membranes and is encoded by petA gene. A hydrophobic stretch next to N-terminus of the protein is considered as a transmembrane anchor for the Rieske protein. It had been shown that the Rieske protein is involved in the quinol binding. In plants it is essential protein for photoautotrophism. Confers resistance to photo-oxidative damages by contributing to the thermal dissipation of light energy and to lumenal acidification (increase of pH gradient). Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.

Function : Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.

The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex (By similarity).

Subunit : The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1).

Subcellular Location : Mitochondrion inner membrane.

Similarity : Contains 1 Rieske domain.

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★質(zhì)子梯度調(diào)節(jié)蛋白1抗體說明書 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測序、載體構(gòu)建等

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