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去泛素化酶22抗體品牌 英文名稱  Anti-USP22

中文名稱  去泛素化酶22抗體品牌 

別    名 Deubiquitinating enzyme 22; Ubiquitin carboxyl-terminal hydrolase 22; Ubiquitin thioesterase 22; Ubiquitin thiolesterase 22; Ubiquitin thiolesterase 8; Ubiquitin thiolesterase 8; Ubiquitin-specific processing protease 8; Ubiquitin-specific processing protease 8; Ubiquitin-specific-processing protease 22; UBP22_HUMAN; Usp22; USP3L.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, Sheep, Xenopus laevis

產(chǎn)品類型   一抗  

研究領(lǐng)域     細胞生物 干細胞 細胞分化 表觀遺傳學(xué) 泛素

蛋白分子量  predicted molecular weight: 60kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human USP22

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

去泛素化酶22抗體品牌 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP22 (ubiquitin specific peptidase 22), also known as USP3L, is a 525 amino acid protein that contains one UBP-type zinc finger and functions to catalyze the conversion of a ubiquitin C-terminal thioester to free ubiquitin and thiol, a reaction that may influence several cellular processes. Via its catalytic activity, USP22 is thought to play an important role in cell cycle progression and may also serve as a cancer stem cell marker.

Function : Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression.

Subunit : Component of some SAGA transcription coactivator-HAT complexes, at least composed of ATXN7, ATXN7L3, ENY2, GCN5L2, SUPT3H, TAF10, TRRAP and USP22. Within the SAGA complex, ATXN7L3, ENY2 and USP22 form a subcomplex required for histone deubiquitination. Interacts directly with ATXN7L3; leading to its recritment to the SAGA complex.

Subcellular Location : Nucleus.

Tissue Specificity : Moderately expressed in various tissues including heart and skeletal muscle, and weakly expressed in lung and liver.

Similarity : Belongs to the peptidase C19 family. UBP8 subfamily.

Contains 1 UBP-type zinc finger.

Database links : UniProtKB/Swiss-Prot: Q9UPT9.2

實驗流程：

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