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產(chǎn)地 | 進口、國產(chǎn) |
品牌 | 上海莼試 |
保存條件 | Store at -20 °C |
貨號 | CS11946 |
應用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 |
CAS編號 | |
抗體名 | Anti-TRPC1 |
克隆性 | |
靶點 | 詳見說明書 |
適應物種 | 詳見說明書 |
形態(tài) | 詳見說明書 |
宿主 | 詳見說明書 |
亞型 | IgG |
標識物 | 詳見說明書 |
濃度 | 1mg/1ml% |
免疫原 | KLH conjugated synthetic peptide derived from human TRPC1 |
產(chǎn)品訂購信息:
英文名稱 Anti-TRPC1
中文名稱 瞬時受體電位通道1抗體規(guī)格
別 名 TRPC1_HUMAN; Short transient receptor potential channel 1; TrpC1; Transient receptor protein 1; TRP-1; TRP 1; TRP 1 protein; TRP1; TRP1 protein; . HTRP 1; HTRP1; MGC133334; MGC133335; Mtrp1; Transient receptor potential cation channel subfamily C member 1; Transient receptor potential channel 1; Trp related protein 1; TRPC1; Trpc1 transient receptor potential cation channel subfamily C member 1.
濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit
產(chǎn)品類型 一抗
研究領域
蛋白分子量 predicted molecular weight: 87kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human TRPC1
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
瞬時受體電位通道1抗體規(guī)格 產(chǎn)品應用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
(石蠟切片需做抗原修復)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 The protein encoded by this gene is a membrane protein that can form a non-selective channel permeable to calcium and other cations. The encoded protein appears to be induced to form channels by a receptor tyrosine kinase-activated phosphatidylinositol second messenger system and also by depletion of intracellular calcium stores. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011].
Function : Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Seems to be also activated by intracellular calcium store depletion.
Subunit : Interacts with TRPC4AP (By similarity). Homotetramer and heterotetramer with TRPC4 and/or TRPC5. Interacts with TRPC3, TRPC4 and TRPC5. Interacts with ITPR3. Interacts with MX1 and RNF24. Interacts with FKBP4.
Subcellular Location : Membrane; Multi-pass membrane protein.
Tissue Specificity : Seems to be ubiquitous.
Similarity : Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC1 sub-subfamily.
Contains 4 ANK repeats.
Database links : UniProtKB/Swiss-Prot: P48995.1
STAT6(signal transducers and activators of transduction 6) 信號轉(zhuǎn)導和轉(zhuǎn)錄激活因子6抗原Multi-class antibodies規(guī)格: 0.5mg
Anti-BRCA2 癌易感基因2抗體Multi-class antibodies規(guī)格: 0.1ml
Rhesus antibody Rh PEG10 高表達基因抗體 規(guī)格 0.1ml
CD45RO 1:150 濃縮液 0.1ml 進口分裝
Tuberin 英文名稱: 馬鈴薯球蛋白(性硬化)抗體 0.2ml
CIB1 英文名稱: 鈣整合素結合蛋白1抗體 0.1ml
Anti-BRCA2 癌易感基因2抗體Multi-class antibodies規(guī)格: 0.1ml
Anti-CD304/BDCA-4/NRP-1 /FITC 熒光素標記神經(jīng)纖毛蛋白-1抗體IgGMulti-class antibodies規(guī)格: 0.2ml
Anti-Annexin V/PE 熒光素PE標記兔抗人、大、小鼠、Annexin V抗體IgGMulti-class antibodies規(guī)格: 0.2ml
鼠抗人CD3單克隆抗體 Anti-CD3 0.1ml
Goat Anti-Mouse IgG/PE-Cy5.5 PE-Cy5.5標記的羊抗小鼠IgG 0.1ml
FEZ2 英文名稱: 椎骨蛋白2抗體 0.2ml
Rhesus antibody Rh PLGF 胎盤生長因子抗體 規(guī)格 0.1ml
Anti-Annexin V/PE 熒光素PE標記兔抗人、大、小鼠、Annexin V抗體IgGMulti-class antibodies規(guī)格: 0.2ml
Anti-TRF/FITC 熒光素標記抗轉(zhuǎn)鐵蛋白抗體IgGMulti-class antibodies規(guī)格: 0.2ml
SM-MHC(Cardiac myosin heavy chain) 心肌肌凝蛋白多肽Multi-class antibodies規(guī)格: 0.5mg
惡性特異性生長因子抗體 Anti-TSGF 0.1ml
SASS6 英文名稱: 紡錘體組裝異常蛋白6抗體 0.2ml
EPHA10 英文名稱: 酪酸蛋白激酶受體A10抗體 0.1ml
Rhesus antibody Rh phospho-IRF3(Ser386) 磷酸化干擾素調(diào)節(jié)因子3抗體 規(guī)格 0.1ml
SM-MHC(Cardiac myosin heavy chain) 心肌肌凝蛋白多肽Multi-class antibodies規(guī)格: 0.5mg
大鼠磷酸化核因子-κB(p-NF-κB)ELISA試劑盒 ,英文名: p-NF-κB ELISA Kit
人血小板活化因子(PAF)ELISA檢測試劑盒Humanplateletactivatingfactor,PAFELISAKit 96T/48T
鯊魚皮質(zhì)醇(Coisol)免疫試劑盒 Shark Coisol ELISA Kit
CLIAKitforOVAsIgE(HumanovalbuminspecificIgE)ELISAKit人卵清蛋白特異性IgE規(guī)格:48T/96T
臺桌清潔劑(飯桌、工作臺、學生課桌椅等)500毫升/瓶
ELISAKitIL-1α人白介素1α規(guī)格:48T/96T
大鼠表面活性物質(zhì)關聯(lián)蛋白J(SPJ)ELISA試劑盒 ,英文名: SPJ ELISA Kit
Human alpha sarcomeric actin (alpha -SCA) ELISA Kit 人α橫紋肌肌動蛋白(α-SCA)ELISA試劑盒
RatcoagulationfactorⅩⅢ,FⅩⅢELISAKit 大鼠凝血因子ⅩⅢ(FⅩⅢ)ELISA試劑盒 96T/48T 進口分裝
CLIAKitforCoisolELISAKit大鼠皮質(zhì)醇規(guī)格:48T/96T
細胞乙脫氫酶2活性比色法定量檢測試劑盒20次
RatcoagulationfactorⅫ,FⅫELISAKit大鼠凝血因子Ⅻ(FⅫ)ELISA試劑盒規(guī)格:96T/48T
瞬時受體電位通道1抗體規(guī)格 大鼠蛋白C(PROC)ELISA試劑盒 ,英文名: PROC ELISA Kit
Rat soluble ierleukin 2 receptor beta chain (IL-2sR beta) ELISA Kit 大鼠白介素2可溶性受體β鏈(IL-2sRβ)ELISA試劑盒
RatprocollagenⅢN-terminalpeptide,PⅢELISAKit 大鼠Ⅲ型前膠原氨基端肽(PⅢ)ELISA試劑盒 96T/48T 進口分裝
CLIAKitforCTX(Humancytotoxin)ELISAKit人細胞毒素規(guī)格:48T/96T
細胞衰老特異性早期脂褐素蘇丹黑原位染色試劑盒(與紅細胞無法分別)50次
ratEsadiolreceptor,ERELISAKit大鼠雌二醇受體(ER)ELISA試劑盒規(guī)格:96T/48T
抗體的生物素化標記實驗要點:
1. 瞬時受體電位通道1抗體規(guī)格 如在反應混合液中有疊氮鈉或游離氨基存在,會抑制標記反應。因此,蛋白質(zhì)在反應前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析;
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應先用幾個不同的分子比來篩選最適條件;
3.用NHSB量過量也是不利的,抗原的結合位點可能因此被封閉,導致抗體失活;
4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等;
5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結合力或活性;
6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結合;
7.交聯(lián)反應后,應充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結合產(chǎn)生競爭作用;
8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導致高本底。
抗體的鑒定:
1)瞬時受體電位通道1抗體規(guī)格 抗體的效價鑒定:不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應,瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。
2)抗體的特異性鑒定:抗體的特異性是指與相應抗原或近似抗原物質(zhì)的識別能力??贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應率來表示。交叉反應率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結合率,求出各自在IC50時的濃度,并按公式計算交叉反應率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應率近似為0,即該血清的特異性較好。
3)抗體親和力:是指抗體和抗原結合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結合位點與抗原決定簇之間立體構型的合適度決定的。有助于維持抗原抗體復合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。