技術(shù)外包服務(wù):

★TWIST蛋白抗體規(guī)格 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測序、載體構(gòu)建等

★蛋白工程：原核、哺乳動物蛋白表達系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細胞工程：細胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細胞培養(yǎng)、細胞膜制備、穩(wěn)定細胞株構(gòu)建、細胞RNAi技術(shù)等等。

實驗流程：

TWIST蛋白抗體規(guī)格 英文名稱  Anti-TWIST protein

中文名稱  TWIST蛋白抗體規(guī)格 

別    名 Twist1; bHLHa38; M-Twist; pdt; Ska; Twist; Twist related protein 1; Twist1.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat

產(chǎn)品類型   一抗  

研究領(lǐng)域     免疫學(xué) 神經(jīng)生物學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子

蛋白分子量  predicted molecular weight: 23kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human TWIST (101-140aa)

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

TWIST蛋白抗體規(guī)格 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. [provided by RefSeq, Jul 2008].

Function : Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.

Subunit : Efficient DNA binding requires dimerization with another bHLH protein. Homodimer or heterodimer with E proteins such as TCF3. ID1 binds preferentially to TCF3 but does not interact efficiently with TWIST1 so ID1 levels control the amount of TCF3 available to dimerize with TWIST1 and thus determine the type of dimer formed.

Subcellular Location : Nucleus.

Tissue Specificity : Subset of mesodermal cells

DISEASE : Defects in TWIST1 are a cause of Saethre-Chotzen syndrome (SCS) [MIM:101400]; also known as acrocephalosyndactyly type 3 (ACS3). SCS is a craniosynostosis syndrome characterized by coronal synostosis, brachycephaly, low frontal hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.

Defects in TWIST1 are the cause of Robinow-Sorauf syndrome (RSS) [MIM:180750]; also known as craniosynostosis-bifid hallux syndrome. RSS is an autosomal dominant defect characterized by minor skull and limb anomalies which is very similar to Saethre-Chotzen syndrome.

Defects in TWIST1 are the cause of craniosynostosis type 1 (CRS1) [MIM:123100]. Craniosynostosis consists of premature fusion of one or more cranial sutures, resulting in an abnormal head shape.

Similarity : Contains 1 basic helix-loop-helix (bHLH) domain.

Database links : UniProtKB/Swiss-Prot: Q15672.1

轉(zhuǎn)錄因子

Twist蛋白是屬于堿性螺旋-環(huán)-螺旋蛋白家族中的高度保守的轉(zhuǎn)錄因子,Twist在凋亡,的轉(zhuǎn)移發(fā)揮一定的作用。

全、新、優(yōu)、品、好四大特點：

TWIST蛋白抗體規(guī)格 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標準品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

好：我公司具有優(yōu)質(zhì)的技術(shù)團隊，產(chǎn)品一旦售出，實驗過程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時沒有任何的后顧之憂。

正在熱銷的相關(guān)產(chǎn)品：

VASH1(Vasohibin 1) 兔抑制蛋白1抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-procaspase 3 半胱天冬酶-3酶原抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh PCNA [Proliferation Marker] 增殖細胞核抗原抗體 規(guī)格 0.1ml

GRN Kit Human 人 Granulin / GRN / Progranulin ELISA配對抗體 ELISA

TRPM1 英文名稱： 瞬時受體電位離子通道蛋白1抗體(M亞家族) 0.1ml

Cdc6 英文名稱： 細胞分裂周期蛋白6抗體 0.1ml

Anti-procaspase 3 半胱天冬酶-3酶原抗體Multi-class antibodies規(guī)格： 0.2ml

sEPCR ELISA Kit 大鼠可溶性內(nèi)皮細胞蛋白C受體Multi-class antibodies規(guī)格： 48T

Anti-AR(phospho Ser213/Ser791)/FITC 熒光素標記磷酸化雄激素受體抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh FAM156A/TMEM2 跨膜蛋白29抗體 規(guī)格 0.2ml

TPO-Ab ELISA Kit 大鼠抗甲狀腺過氧化物酶抗體 96T

Myf6 英文名稱： 生肌調(diào)節(jié)因子6抗體 0.2ml

phospho-ACTH (Ser168) 英文名稱： 磷酸化促腎上腺皮質(zhì)激素ACTH抗體 0.1ml

Anti-AR(phospho Ser213/Ser791)/FITC 熒光素標記磷酸化雄激素受體抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Vimentin/FITC 熒光素標記波形蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

CGB(Chromogranin B) 嗜鉻粒素B抗原Multi-class antibodies規(guī)格： 0.5mg

周期素依賴激酶1抗體 Anti-Cdc2/P34CDC2/CDK1 0.1ml

SUFU/Suppressor of Fused 英文名稱： 抑制Hh信號通路蛋白SUFU抗體 0.2ml

ESAT6 英文名稱： 結(jié)核分枝桿菌分泌性蛋白ESAT6抗體 0.2ml

Rhesus antibody Rh phospho-GADD153 (Ser30) 磷酸化GADD153抗體 規(guī)格 0.1ml

CGB(Chromogranin B) 嗜鉻粒素B抗原Multi-class antibodies規(guī)格： 0.5mg

大鼠腦肽前體N末端片段(-proBNP)ELISA試劑盒 ，英文名： -proBNP ELISA Kit

人S100B蛋白(S-100B)ELISA檢測試劑盒HumanSolubleprotein-100B,S-100BELISAKit 96T/48T

魚11-酮類固醇免疫試劑盒 Fish 11-Keto Steroid ELISA Kit

CLIAKitforPAPELISAKit大鼠纖溶酶抗纖溶酶復(fù)合物規(guī)格：48T/96T

水樣品氟元素比色法定量檢測試劑盒20次

ELISAKitMN人變規(guī)格：48T/96T

大鼠成纖維生長因子受體底物2(FRS2)ELISA試劑盒 ，英文名： FRS2 ELISA Kit

Human ai alpha 1 chymoypsin (AACT) ELISA Kit 人α1抗胰糜蛋白酶(AACT)ELISA試劑盒

RatCollageypeⅡ,ColⅡELISAKit 大鼠Ⅱ型膠原(ColⅡ)ELISA試劑盒 96T/48T 進口分裝

CLIAKitforCr(HumanCrosslaps)ELISAKit人骨膠原交聯(lián)規(guī)格：48T/96T

細胞血紅素氧合酶(HEMEOXYGENASE)活性熒光定量檢測試劑盒20次

RatCyclin-D3ELISAKit大鼠細胞周期素D3(Cyclin-D3)ELISA試劑盒規(guī)格：96T/48T

TWIST蛋白抗體規(guī)格 大鼠毒蕈堿型膽堿受體M2(CHRM2)ELISA試劑盒 ，英文名： CHRM2 ELISA Kit

Rat ierleukin 17 (IL-17) ELISA Kit 大鼠白介素17(IL-17)ELISA試劑盒

RatOsteoprotegerin,OPGELISAKIT 大鼠骨保護素(OPG)ELISA試劑盒 96T/48T 進口分裝

CLIAKitforD2RELISAKit大鼠多巴胺D2受體規(guī)格：48T/96T

細胞色素P450亞酶2B6(BUPROPION)活性高效液相色譜法(HPLC)定量檢測試劑盒20次

Ratglucocoicoidreceptor-α,GR-αELISAKit大鼠糖皮質(zhì)激素受體α(GR-α)ELISA試劑盒規(guī)格：96T/48T