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產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
品牌 | 上海莼試 |
保存條件 | Store at -20 °C |
貨號(hào) | CS11856 |
應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
CAS編號(hào) | |
抗體名 | Anti-Transferrin |
克隆性 | |
靶點(diǎn) | 詳見(jiàn)說(shuō)明書(shū) |
適應(yīng)物種 | 詳見(jiàn)說(shuō)明書(shū) |
形態(tài) | 詳見(jiàn)說(shuō)明書(shū) |
宿主 | 詳見(jiàn)說(shuō)明書(shū) |
亞型 | IgG |
標(biāo)識(shí)物 | 詳見(jiàn)說(shuō)明書(shū) |
濃度 | 1mg/1ml% |
免疫原 | human Transferrin in plasma |
產(chǎn)品訂購(gòu)信息:
英文名稱(chēng) Anti-Transferrin
中文名稱(chēng) 轉(zhuǎn)鐵蛋白/血清鐵傳遞蛋白抗體說(shuō)明書(shū)
別 名 Apotransferrin; Beta 1 metal binding globulin; DKFZp781D0156; PRO1400; PRO1557; PRO2086; Serotransferrin precursor; Siderophilin; TF; Transferin; Transferrin; TRFE_HUMAN; Beta-1 metal-binding globulin; Serotransferrin.
濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg 0.2ml/200μg 1ml/1mg
抗體來(lái)源 Rabbit
克隆類(lèi)型 polyclonal
交叉反應(yīng) Human
產(chǎn)品類(lèi)型 一抗
研究領(lǐng)域 心
蛋白分子量 predicted molecular weight: 77kDa
性 狀 Lyophilized or Liquid
免 疫 原 human Transferrin in plasma
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
轉(zhuǎn)鐵蛋白/血清鐵傳遞蛋白抗體說(shuō)明書(shū) 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq, Sep 2009].
Function : Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
Subunit : Monomer.
Subcellular Location : Secreted.
Tissue Specificity : Expressed by the liver and secreted in plasma.
DISEASE : Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
Similarity : Belongs to the transferrin family.
Contains 2 transferrin-like domains.
Database links : UniProtKB/Swiss-Prot: P02787.3
Ag-ARC(ARG3.1/ARC, Activity-regulated cytoskeleton-associated protein) ARC(多肽抗原)Multi-class antibodies規(guī)格: 0.5mg
Anti-Phospho-Beta-Catenin (Ser45) 磷酸化β 連環(huán)素蛋白抗體Multi-class antibodies規(guī)格: 0.1ml
Rhesus antibody Rh PCDHGB2 原鈣粘蛋白γB2抗體 規(guī)格 0.2ml
AHSG Kit Human 人 Fetuin-A / AHSG / FETUA ELISA配對(duì)抗體 ELISA
TRIP13 英文名稱(chēng): 甲狀腺激素受體相互作用13/瘤病毒16型E1結(jié)合蛋白抗體 0.2ml
phospho-CBL2 (Tyr774) 英文名稱(chēng): 磷酸化原癌蛋白CBL2抗體 0.1ml
Anti-Phospho-Beta-Catenin (Ser45) 磷酸化β 連環(huán)素蛋白抗體Multi-class antibodies規(guī)格: 0.1ml
ADP ELISA Kit 大鼠脂聯(lián)素Multi-class antibodies規(guī)格: 48T
Anti-Aromatase P450/FITC 熒光素標(biāo)記芳香化酶/細(xì)胞色素P450/P450抗體IgGMulti-class antibodies規(guī)格: 0.2ml
Rhesus antibody Rh FAM129A/Cell growth inhibiting gene 39 protein 細(xì)胞生長(zhǎng)抑制基因39蛋白抗體 規(guī)格 0.2ml
ANG-4(Mouse Angiopoietin 4) ELISA Kit 小鼠生成素4 96T
MRP2 英文名稱(chēng): 多藥耐藥相關(guān)蛋白2抗體 0.1ml
AMBN 英文名稱(chēng): 釉基質(zhì)蛋白抗體 0.2ml
Anti-Aromatase P450/FITC 熒光素標(biāo)記芳香化酶/細(xì)胞色素P450/P450抗體IgGMulti-class antibodies規(guī)格: 0.2ml
Anti-Phospho-SirT1 (Ser47) /FITC 熒光素標(biāo)記磷酸化沉默調(diào)節(jié)蛋白1抗體IgGMulti-class antibodies規(guī)格: 0.2ml
Bax peptide Bax(多肽抗原)Multi-class antibodies規(guī)格: 0.5mg
軸索過(guò)度生長(zhǎng)抑制因子-B抗體 Anti-Nogo-B 0.2ml
Synapsin II 英文名稱(chēng): 神經(jīng)突觸素2抗體 0.2ml
ESYT1 英文名稱(chēng): 延伸突觸蛋白1抗體 0.2ml
Rhesus antibody Rh phospho-P53(Thr18) 磷酸化抑制基因P53抗體 規(guī)格 0.1ml
Bax peptide Bax(多肽抗原)Multi-class antibodies規(guī)格: 0.5mg
大鼠內(nèi)皮型合成酶(eNOS/NOS3)ELISA試劑盒 ,英文名: eNOS/NOS3 ELISA Kit
人中期因子(MK)ELISA檢測(cè)試劑盒HumanMidkine,MKELISAKit 96T/48T
魚(yú)白介素4(IL-4)免疫試劑盒 Fish Ierleukin 4,IL-4 ELISA Kit
CLIAKitforPC(HumanplasmaaicoagulaproteinC)ELISAKit人血漿抗凝蛋白C規(guī)格:48T/96T
雙參數(shù)細(xì)胞周期G2期流式細(xì)胞分析試劑盒10次
ELISAKitM2-PK人同酸激酶M2型同工酶規(guī)格:48T/96T
大鼠成纖維細(xì)胞生長(zhǎng)因子7(FGF7)ELISA試劑盒 ,英文名: FGF7 ELISA Kit
TH glycoprotein (THP) ELISA Kit 人TH糖蛋白(THP)ELISA試劑盒
Ratmaixmetalloproteinase5,MMP-5ELISAKit 大鼠基質(zhì)金屬蛋白酶5(MMP-5)ELISA試劑盒 96T/48T 進(jìn)口分裝
CLIAKitforCsA(HumanCyclosporineA)ELISAKit人環(huán)孢素A規(guī)格:48T/96T
細(xì)胞線粒體NADH氧化酶活性定量檢測(cè)試劑盒20次
RatDihydrotestosterone,DHTELISAKit大鼠雙氫睪同(DHT)ELISA試劑盒規(guī)格:96T/48T
轉(zhuǎn)鐵蛋白/血清鐵傳遞蛋白抗體說(shuō)明書(shū) 大鼠多巴胺轉(zhuǎn)運(yùn)蛋白(DAT)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝
人腦腸肽(BGP/Gehrelin)免疫試劑盒 Human brain-g peptides,BGP/Gehrelin Elisa kit
MousesolubleClusterofdiffereiation86,sCD86ELISAKit小鼠可溶性CD86(B7-2/sCD86)ELISA試劑盒規(guī)格:96T/48T
冰凍切片組織TUBULIN蛋白表達(dá)NBT顯色光學(xué)顯微鏡檢測(cè)試劑盒10/20次
Humanplasmin/fibrinolysin,PL/FbnELISA試劑盒人纖溶酶/纖維蛋白溶酶(PL/Fbn)ELISA試劑盒規(guī)格:96T/48T
Humanspleeyrosinekinase,SykELISAKit人*酪氨酸激酶(Syk)ELISA試劑盒規(guī)格:96T/48T
抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn):
1. 轉(zhuǎn)鐵蛋白/血清鐵傳遞蛋白抗體說(shuō)明書(shū) 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析;
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件;
3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活;
4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等;
5.當(dāng)游離ε-氨基(賴(lài)氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性;
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合;
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用;
8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。
抗體的鑒定:
1)轉(zhuǎn)鐵蛋白/血清鐵傳遞蛋白抗體說(shuō)明書(shū) 抗體的效價(jià)鑒定:不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。
2)抗體的特異性鑒定:抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力??贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3)抗體親和力:是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol??贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。