金屬蛋白酶組織抑制因子-4抗體規(guī)格 英文名稱  Anti-TIMP-4

中文名稱  金屬蛋白酶組織抑制因子-4抗體規(guī)格 

別    名 Metalloproteinase inhibitor 4; TIMP 4; TIMP4; TIMP metallopeptidase inhibitor 4; TIMP-4; Timp4; TIMP4_HUMAN; Tissue inhibitor of metalloproteinase 4.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來(lái)源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Cow, Horse, Rabbit

產(chǎn)品類型   一抗  

研究領(lǐng)域     心 神經(jīng)生物學(xué) 合成與降解

蛋白分子量  predicted molecular weight: 22kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human TIMP-4

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

金屬蛋白酶組織抑制因子-4抗體規(guī)格 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The tissue inhibitors of metalloproteinases (TIMPs) are naturally occurring proteins that specifically inhibit matrix metalloproteinases and regulate extracellular matrix turnover and tissue remodeling by forming tightbinding inhibitory complexes with the MMPs. Thus, TIMPs maintain the balance between matrix destruction and formation. An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. The TIMP proteins share several structural features including six loops held in place by six disulfide bonds arranged in three knot-like structures. These proteins also contain twelve cysteine residues in conserved regions of the molecule that form six disulfide bonds, essential for the formation of native conformations, and the N terminal region that is necessary for inhibitory activities. The N terminus of each TIMP contains a consensus sequence (VIRAK) and each TIMP is translated with a 29 amino acid leader sequence that is cleaved off to produce the mature protein. The C terminal regions are divergent, which enhances the selectivity of inhibition and binding efficiency. Although the TIMP proteins share high homology, they may either be secreted extracellularly in soluble form (TIMP1, TIMP2 and TIMP4) or bind to extracellular matrix components (TIMP3).

The MMPs and TIMPs can be divided into two groups with respect to gene expression: the majority exhibit inducible expression and a small number are produced constitutively or are expressed at very low levels and are not inducible. Among agents that induce MMP and TIMP production are the inflammatory cytokines TNF alpha and IL1 beta. A marked cell type specificity is a hallmark of both MMP and TIMP gene expression (i.e. a limited number of cell types can be induced to make these proteins). Tissue Inhibitor of Metalloproteinases 4 (TIMP4) was identified by molecular cloning. TIMP4 shows 37 % amino acid identity with TIMP1 and 51 % homology with TIMP2 and TIMP3. TIMP4 is secreted extracellularly, predominantly in heart and brain tissue. It may function in a tissue specific fashion in extracellular matrix (ECM) homeostasis. TIMP4 has a strong inhibitory effect on the invasion of human breast cancer cells across reconstituted basement membranes suggesting that TIMP4 may have an important role in inhibiting primary tumor growth and progression. The human TIMP4 gene has the chromosomal location of 3p25.

Function : Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.

Subcellular Location : Secreted.

Tissue Specificity : Abundant in heart and present at low levels in many other tissues.

Similarity : Belongs to the protease inhibitor I35 (TIMP) family.

Contains 1 NTR domain.

Database links : UniProtKB/Swiss-Prot: Q99727.1

合成與降解（Synthesis and Degradation）

TIMP-4是TIMP家族的新成員，TIMP-3與TIMP-4同樣具有較強(qiáng)的促凋亡、抑制生成、浸潤(rùn)作用。

實(shí)驗(yàn)流程：

全、新、優(yōu)、品、好四大特點(diǎn)：

金屬蛋白酶組織抑制因子-4抗體規(guī)格 全：公司提供上萬(wàn)種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過(guò)程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時(shí)沒(méi)有任何的后顧之憂。

正在熱銷的相關(guān)產(chǎn)品：

Tyrosinase 酪氨酸酶(多肽抗原)Multi-class antibodies規(guī)格： 0.5mg

Anti-CD337/NKp30/NCR3 細(xì)胞毒性受體NK-p30抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Orexin-A 增食欲素A/欲激素A 規(guī)格 0.1ml

CD300C Kit Human 人 CD300C ELISA配對(duì)抗體 ELISA

TRAF4 英文名稱： 壞死因子受體相關(guān)蛋白4抗體 0.1ml

C1orf98 英文名稱： 1號(hào)染色體開放閱讀框98抗體 0.2ml

Anti-CD337/NKp30/NCR3 細(xì)胞毒性受體NK-p30抗體Multi-class antibodies規(guī)格： 0.2ml

TSH ELISA Kit 大鼠促甲狀腺素Multi-class antibodies規(guī)格： 48T

Anti-ATG1/ULK1/FITC 熒光素標(biāo)記自噬相關(guān)蛋白1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Factor XI 凝血因子11抗體 規(guī)格 0.2ml

MUC5AC(Mouse Mucin-5 subtype AC) ELISA Kit 小鼠粘蛋白/粘液素5AC 96T

MGMT 英文名稱： O6甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶抗體 0.1ml

ACCN1 英文名稱： 腦通道蛋白1抗體 0.2ml

Anti-ATG1/ULK1/FITC 熒光素標(biāo)記自噬相關(guān)蛋白1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Telomerase Binding Protein，P23/FITC 熒光素標(biāo)記端粒酶結(jié)合蛋白P23抗體IgGMulti-class antibodies規(guī)格： 0.2ml

M2-PK ( pyruvate Kinase M2) 酸激酶-M2(抗原)Multi-class antibodies規(guī)格： 0.5mg

抗豬病大腸桿菌志賀樣毒素Ⅱ型突變體抗體 Shiga-like toxin IIe variant subunit A[Escherichia coli] 0.2ml

phospho-STAT6 (Tyr641) 英文名稱： 磷酸化信號(hào)轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子6抗體 0.1ml

EYA4 英文名稱： EYA4蛋白抗體 0.2ml

Rhesus antibody Rh phospho-M-CSF Receptor(Tyr809) 磷酸化巨噬細(xì)胞集落刺激因子受體抗體 規(guī)格 0.1ml

M2-PK ( pyruvate Kinase M2) 酸激酶-M2(抗原)Multi-class antibodies規(guī)格： 0.5mg

大鼠凝血酶原片段F1+2(F1+2)ELISA試劑盒 ，英文名： F1+2 ELISA Kit

人抗天然脫氧核糖核酸抗體(n-DNA-Ab)ELISA檢測(cè)試劑盒Humannaturaldeoxyribonucleicacidaibody,n-DNA-AbELISAKit 96T/48T

魚金屬硫蛋白(MT)免疫試劑盒 Fish metallothionein,MT ELISA kit

CLIAKitforPDHE1(Humanpyruvatedehydrogenase-E1)ELISAKit人同酸脫氫酶E1規(guī)格：48T/96T

食油檸檬酸比色法定量檢測(cè)試劑盒20次

ELISAKitOPAs人不透光相關(guān)蛋白規(guī)格：48T/96T

大鼠促生長(zhǎng)激素釋放激素(GHRH)ELISA試劑盒 ，英文名： GHRH ELISA Kit

Human substance P (SP) ELISA Kit 人p物質(zhì)(SP)ELISA試劑盒

RatImmunoglobulinG,IgGELISAKit 大鼠免疫球蛋白G(IgG)ELISA試劑盒 96T/48T 進(jìn)口分裝

CLIAKitforCTACK/CCL27(HumancaneousTcell-atactingchemokine,CTACK)ELISAKit人皮膚T細(xì)胞虜獲趨化因子規(guī)格：48T/96T

細(xì)胞糖原酶連續(xù)循環(huán)反應(yīng)比色法定量檢測(cè)試劑盒20次

RatEndothelialniicoxidesyhase3,eNOS-3ELISAKit大鼠內(nèi)皮型合成酶3(eNOS-3)ELISA試劑盒規(guī)格：96T/48T

金屬蛋白酶組織抑制因子-4抗體規(guī)格 大鼠肥大細(xì)胞類糜蛋白酶1(CMA1)ELISA試劑盒 ，英文名： CMA1 ELISA Kit

Rat gamma aminobyric acid (GABA) ELISA Kit 大鼠γ氨基(GABA)ELISA試劑盒

Ratkeratinocyteaocrinefactor/Amphiregulin,KAF/ARELISAKit 大鼠角化細(xì)胞因子(KAF)/雙調(diào)蛋白(AR)ELISA試劑盒 96T/48T 進(jìn)口分裝

CLIAKitforDPD(Mousedeoxypyridinoline)ELISAKit小鼠脫氧酚/脫氧啉規(guī)格：48T/96T

細(xì)胞羥賴氨酸(hydroxylisine)含量比色法定量檢測(cè)試劑盒20次

RatHelicobacterpyloriIgG,Hp-IgGELISAKit大鼠幽門螺旋菌IgG(Hp-IgG)ELISA試劑盒規(guī)格：96T/48T

技術(shù)外包服務(wù):

★金屬蛋白酶組織抑制因子-4抗體規(guī)格 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等

★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。