產(chǎn)品訂購信息：
英文名稱  Anti-TOP2 alpha 

中文名稱   DNA拓普西異構(gòu)酶ⅡA抗體品牌 

別    名  Topoisomerase Ⅱalpha; DNA topoisomerase II, alpha isozyme; DNA topoisomerase 2 alpha; DNA topoisomerase II 170 kD; DNA Topoisomerase2; TOP2; TOP2A; TopII alpha; Topo II alpha; Topoisomerase DNA II alpha 170kDa; Topoisomerase II alpha 170 kDa; TP2A; TPⅡA; TOP2A_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit 

產(chǎn)品類型   一抗  

研究領(lǐng)域     染色質(zhì)和核信號 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子

蛋白分子量  predicted molecular weight: 174kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human TOP2 alpha (592-643aa) 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

DNA拓普西異構(gòu)酶ⅡA抗體品牌 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Function : Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.

Subunit : Homodimer. Interacts with COPS5.

Subcellular Location : Cytoplasm. Nucleus, nucleoplasm. Note=Generally located in the nucleoplasm.

Post-translational modifications : Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.

Similarity : Belongs to the type II topoisomerase family.

Database links : UniProtKB/Swiss-Prot: P11388.3

Eotaxin-2 human 人噬酸性粒細(xì)胞趨化因子-2Multi-class antibodies

Anti-phospho C-Met/HGFR(Tyr1365) 磷酸化原癌基因c-Met抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh NPC1/Niemann Pick C1 尼曼匹克C1前體蛋白抗體 規(guī)格 0.2ml

Col I (Human Collagen Type I ) ELISA Kit 人Ⅰ型膠原 96T

TPX2 英文名稱： 微管相關(guān)同源蛋白TPX2抗體 0.2ml

C6orf225 英文名稱： 6號染色體開放閱讀框225抗體 0.2ml

Anti-phospho C-Met/HGFR(Tyr1365) 磷酸化原癌基因c-Met抗體Multi-class antibodies規(guī)格： 0.1ml

PEPT2 (Peptide-transporters 2) 腸道肽轉(zhuǎn)運(yùn)蛋白2/小肽轉(zhuǎn)運(yùn)蛋白2抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-ATP1b2/Na+K+ATPase ATP酶通道蛋白抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh phospho-Alpha synuclein(Tyr133) 磷酸化核突觸蛋白α抗體 規(guī)格 0.1ml

Ostepontin 濃縮液 0.1ml 進(jìn)口分裝

Urocortin 英文名稱： 尿皮質(zhì)素抗體 0.2ml

DEDD1 英文名稱： 死亡效應(yīng)結(jié)構(gòu)域蛋白1抗體 0.2ml

Anti-ATP1b2/Na+K+ATPase ATP酶通道蛋白抗體Multi-class antibodies規(guī)格： 0.2ml

Anti-PTPRJ/CD148/DEP1/FITC 熒光素標(biāo)記CD148抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Recomb Protein A 重組蛋白AMulti-class antibodies規(guī)格： 1mg

粘附分子整合素α5抗體 Anti-ITG-α5/CD49e 0.2ml

NTR3 英文名稱： 神經(jīng)降壓素受體3抗體(糖蛋白95) 0.2ml

FAAH1 英文名稱： 脂肪酸酰胺水解酶1抗體 0.2ml

Rhesus antibody Rh Phospho-PPIG (Ser376) 磷酸化親環(huán)蛋白(親環(huán)素)PPIG抗體 規(guī)格 0.2ml

Recomb Protein A 重組蛋白AMulti-class antibodies規(guī)格： 1mg

大鼠凝血因子Ⅹ(FⅩ)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

人絲氨酸/蘇氨酸激酶24(STK24)免疫試劑盒 Human Serine/threonine-protein kinase 24,STK24 ELISA Kit

果膠含量試劑盒 可見分光光度法 50管/24樣

RatVascularEndothelialcellGrowthFactor,VEGFELISA試劑盒大鼠內(nèi)皮細(xì)胞生長因子(VEGF)ELISA試劑盒規(guī)格：96T/48T

humanlymphocyteaigen6complexlocusA,Ly6aELISA試劑盒人淋巴細(xì)胞抗原6復(fù)合物基因座A(Ly6a)ELISA試劑盒規(guī)格：96T/48T

HumanparamyxovirusparotitisIgGELISAKit人病毒IgGELISA試劑盒規(guī)格：96T/48T

大鼠激素釋放激素受體(GHR)ELISA試劑盒 ，英文名： GHR ELISA Kit

Porcine histamine (HIS) ELISA Kit 豬組胺(HIS)ELISA試劑盒

ELISA 小鼠腦衍化神經(jīng)營養(yǎng)因子(mouse BDNF) 48T/96T 進(jìn)口分裝

CLIAKitforL-LDH(HumanL-LactateDehydrogenase)ELISAKit人L-乳酸脫氫酶規(guī)格：48T/96T

通用型EBV(EPSTEIN-BARR)病毒定量PCR擴(kuò)增檢測試劑盒20次

ELISAKitCRP雞C反應(yīng)蛋白規(guī)格：48T/96T

DNA拓普西異構(gòu)酶ⅡA抗體品牌 大鼠肺表面活性物質(zhì)相關(guān)蛋白C(SP-C)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

人鳥苷酸交換因子(GEF)免疫試劑盒 Human Guanine Exchange Factor,GEF ELISA Kit

Mousesexhormone-bindingglobulin,SHBGELISAKit小鼠性激素結(jié)合球蛋白(SHBG)ELISA試劑盒規(guī)格：96T/48T

冰凍切片組織RHO蛋白表達(dá)NBT顯色光學(xué)顯微鏡檢測試劑盒10/20次

HumanPlacealalkalinepkosphatase,PLAPELISA試劑盒人胎盤堿性磷酸酶(PLAP)ELISA試劑盒規(guī)格：96T/48T

Humansolubletumornecrosisfactor-relatedapoptosisinducingligand,sAILELISAKit人可溶性壞死因子相關(guān)凋亡誘導(dǎo)配體(sAIL)ELISA試劑盒規(guī)格：96T/48T

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. DNA拓普西異構(gòu)酶ⅡA抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時,或位于酶的催化位點(diǎn)時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）DNA拓普西異構(gòu)酶ⅡA抗體品牌 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗(yàn)測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。