產品訂購信息：
英文名稱  Anti-TLR1 

中文名稱   Toll樣受體1抗體規(guī)格 

別    名  Toll-like receptor 1; GD2:CD281; CD281 antigen; rsc786; Similar to Drosophila Toll protein; TIL; Toll (Drosophila) homolog; Toll like receptor 1 Precursor; Toll/Interleukin 1 receptor like; TLR1_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應   Human, Mouse, Rat, Dog, Cow, Horse 

產品類型   一抗  

研究領域    免疫學 細胞膜受體

蛋白分子量  predicted molecular weight: 87(hu,mo)/91(rat)kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human TLR1 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Toll樣受體1抗體規(guī)格 產品應用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產品介紹 TLR1 is a member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. The gene encoding TLR1 is ubiquitously expressed, and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for this gene.

Function : Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity).

Subunit : Binds MYD88 (via TIR domain). Interacts with CNPY3 (By similarity). Interacts (via extracellular domain) with TLR2. Ligand binding induces the formation of a heterodimer with TLR2.

Subcellular Location : Cell membrane; Single-pass type I membrane protein (By similarity). Cytoplasmic vesicle, phagosome membrane; Single-pass type I membrane protein (By similarity).

Tissue Specificity : Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine.

Similarity : Belongs to the Toll-like receptor family.

Contains 19 LRR (leucine-rich) repeats.

Contains 1 LRRCT domain.

Contains 1 TIR domain.

Database links : UniProtKB/Swiss-Prot: Q15399.3

Anti-Oct-4 /FITC 熒光素標記胚胎干細胞關鍵蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rabbit Anti-human IgG/HRP 辣根過氧化物酶標記兔抗人IgGMulti-class antibodies規(guī)格： 0.1ml

周期素依賴性激酶8抗體 Anti-CDK8 0.2ml

Rabbit Anti-horse IgM/FITC FITC標記的兔抗馬IgM 0.1ml

Factor VII 英文名稱： 凝血因子7抗體 0.1ml

Rhesus antibody Rh Plakophilin 1 橋粒斑菲素蛋白1抗體 規(guī)格 0.2ml

Rabbit Anti-human IgG/HRP 辣根過氧化物酶標記兔抗人IgGMulti-class antibodies規(guī)格： 0.1ml

LH/CG Receptor(Luteinizing Hormone/Choriogonadotropin Receptor) 人促黃體生成素受體抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-Agrin 聚集蛋白抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh phospho-CaMK2 alpha (Tyr231) 磷酸化鈣/鈣調素依賴蛋白激酶2α抗體 規(guī)格 0.1ml

堿性磷酸酶阻斷劑 10ml Sigma原料

VGLUT1/BNP1 英文名稱： 腦特異性依賴無機磷轉運蛋白抗體 0.2ml

DEGS1 英文名稱： 退行性精母細胞同源物1抗體 0.2ml

Anti-Agrin 聚集蛋白抗體Multi-class antibodies規(guī)格： 0.2ml

Anti-Phospho-TBK1/NAK (Ser172) /FITC 熒光素標記NF-κB活化激酶抗體IgGMulti-class antibodies規(guī)格： 0.2ml

N-coR1 (Nuclear receptor co-repressor 1) 核受體輔助抑制因子(抗原)Multi-class antibodies規(guī)格： 0.5mg

磷酸協(xié)同轉運蛋白抗體 SLC34A2/NaPi-2b 0.1ml

SMC6 英文名稱： 染色體結構維持蛋白6抗體 0.2ml

E.coli O157:H7 英文名稱： 性大腸桿菌埃希氏菌O157:H7抗體 0.2ml

Rhesus antibody Rh phospho-MARK4(Ser423) 磷酸化絲氨酸/蘇氨酸蛋白激酶MARK4抗體 規(guī)格 0.1ml

N-coR1 (Nuclear receptor co-repressor 1) 核受體輔助抑制因子(抗原)Multi-class antibodies規(guī)格： 0.5mg

人α平滑肌肌動蛋白(α-SMA)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

兔子白三烯B4(LTB4)免疫試劑盒 Rabbit Leukoiene B4,LTB4 ELISA Kit

絨毛膜激素β(β-HCG)ELISA試劑盒 ，英文名： β-HCG ELISA Kit

Porcinethyroopin-releasinghormone,HELISA試劑盒豬促甲狀腺素釋放激素(H)ELISA試劑盒規(guī)格：96T/48T

Humanai-albuminaibody,AAAELISA試劑盒人抗白蛋白抗體(AAA)ELISA試劑盒規(guī)格：96T/48T

Humanbasicfibroblastgrowthfactor，bFGFELISAKit人堿性成纖維細胞生長因子(bFGF)ELISA試劑盒規(guī)格：96T/48T

大鼠生長激素(GH)ELISA試劑盒 ，英文名： GH ELISA Kit

人垂草扁桃酸(VMA)ELISA檢測試劑盒Humanvanillylmandelicacid,VMAELISAKit 96T/48T

海獅雌二醇(E2)免疫試劑盒 Sea Lion Esadiol,E2 ELISA kit

CLIAKitforPLH催乳素規(guī)格：48T/96T

石蠟切片組織PDGF-A蛋白表達熒光顯微鏡檢測試劑盒10/20次

ELISAKitPMNElastase人多形核白細胞彈性蛋白酶規(guī)格：48T/96T

Toll樣受體1抗體規(guī)格 大鼠透明質酸(HA)ELISA試劑盒 ，英文名： HA ELISA Kit

人胃蛋白酶原A(PG-A)ELISA檢測試劑盒humanPepsinogenA,PG-AELISAKit 96T/48T

倉鼠白介素1β(IL-1β)免疫試劑盒 Hamster Ierleukin 1β,IL-1β ELISA Kit

CLIAKitforPTHrPELISAKit大鼠甲狀旁腺激素相關蛋白規(guī)格：48T/96T

石蠟切片中性脂質油紅O直接染色試劑盒50次

ELISAKitOFQ/N人孤腓肽規(guī)格：48T/96T

抗體的生物素化標記實驗要點：

1. Toll樣受體1抗體規(guī)格 如在反應混合液中有疊氮鈉或游離氨基存在,會抑制標記反應。因此,蛋白質在反應前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質之間的分子比按蛋白質表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結合位點可能因此被封閉,導致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結合；

7.交聯(lián)反應后,應充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結合產生競爭作用；

8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導致高本底。

抗體的鑒定：

1）Toll樣受體1抗體規(guī)格 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應,瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應抗原或近似抗原物質的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應率來表示。交叉反應率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結合率,求出各自在IC50時的濃度,并按公式計算交叉反應率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質的交叉反應率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結合位點與抗原決定簇之間立體構型的合適度決定的。有助于維持抗原抗體復合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。