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環(huán)指蛋白88抗體說明書
  • 品牌:上海莼試
  • 產(chǎn)地:進口、國產(chǎn)
  • 貨號:CS11792
  • 發(fā)布日期: 2019-01-23
  • 更新日期: 2025-03-14
產(chǎn)品詳請
產(chǎn)地 進口、國產(chǎn)
品牌 上海莼試
保存條件 Store at -20 °C
貨號 CS11792
應(yīng)用范圍 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200
CAS編號
抗體名 Anti-TRIM5α/RNF88
克隆性
靶點 詳見說明書
適應(yīng)物種 詳見說明書
形態(tài) 詳見說明書
宿主 詳見說明書
亞型 IgG
標識物 詳見說明書
濃度 1mg/1ml%
免疫原 KLH conjugated synthetic peptide derived from human TRIM5α/RNF88 corresponding to amino acids 351-450.

環(huán)指蛋白88抗體說明書 英文名稱  Anti-TRIM5α/RNF88

中文名稱  環(huán)指蛋白88抗體說明書 

     TRIM5 alpha; RING finger protein 88; RNF88; TRIM5; TRIM5_HUMAN; tripartite motif protein 5; tripartite motif protein TRIM5; Tripartite motif-containing protein 5.

產(chǎn)品屬性:

      1mg/1ml

規(guī)   0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human

產(chǎn)品類型   一抗  

研究領(lǐng)域    細胞生物 免疫學(xué) 表觀遺傳學(xué)

蛋白分子量  predicted molecular weight: 54kDa 

       Lyophilized or Liquid

  KLH conjugated synthetic peptide derived from human TRIM5α/RNF88 corresponding to amino acids 351-450.

      IgG

純化方法   affinity purified by Protein A

   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

環(huán)指蛋白88抗體說明書 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

(石蠟切片需做抗原修復(fù)) 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 TRIM5a is a 493 amino acid member of the large tripartite motif protein (TRIM) family. TRIM proteins are composed of three zinc-binding domains, a RING, a B-box 2 and a coiled-coil domain, and they use homomultimerization to identify different cell compartments. Some TRIM proteins, such as TRIM5? also possess a carboxy-terminal B30.2 (SPRY) domain and localize to the cytoplasm. TRIM5?mediates innate intracellular retroviral resistance, which is dependent on its carboxy-terminal domain. The three variable regions of the B30.2 domain form loops on one side of the B30.2 core structure of TRIM5?which may form a binding surface for the virus. TRIM5?trimerization plays a major role in its affinity for the retroviral capsid, and in its ability to inhibit virus infection. The linker region between the coiled-coil and B30.2 domains of TRIM5?is required for this trimerization. TRIM5?blocks infection after the virus has entered the cell.

Subunit : Isoform Alpha forms homotrimers, and may interact with retroviral CA protein. Isoform Delta interacts with BTBD1 and BTBD2.

Subcellular Location : Cytoplasm, P-body.

Similarity : Belongs to the TRIM/RBCC family.

Contains 1 B box-type zinc finger.

Contains 1 B30.2/SPRY domain.

Contains 1 RING-type zinc finger.

科學(xué)家經(jīng)過研究發(fā)現(xiàn)TRIM5蛋白有阻止HIV繁殖的作用,在毒感染過程中,一旦HIV進入細胞,TRIM5蛋白立刻攔截并阻止其繁殖。因此, TRIM5是先天中對HIV的感應(yīng)器。與適應(yīng)性“后發(fā)制人”不同,先天能夠在接觸到病原體時立刻將其消除。

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技術(shù)外包服務(wù):

環(huán)指蛋白88抗體說明書 分子生物學(xué):質(zhì)粒抽提、PCRQ-PCR、RT-PCR、分子生物學(xué):基因合成、引物合成、基因測序、載體構(gòu)建等

蛋白工程:原核、哺乳動物蛋白表達系統(tǒng)等

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細胞工程:細胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細胞培養(yǎng)、細胞膜制備、穩(wěn)定細胞株構(gòu)建、細胞RNAi技術(shù)等等。


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