產(chǎn)品訂購(gòu)信息：
英文名稱  Anti-phospho-TIRAP(Tyr86) 

中文名稱   磷酸化白細(xì)胞介素1受體銜接蛋白抗體費(fèi)用 

別    名  TIRAP (phospho Y86); Adapter protein wyatt; MAL antibody MyD88 adapter like protein; TIR domain containing adapter protein; Toll interleukin 1 receptor (TIR) domain containing adaptor protein; Toll interleukin 1 receptor domain containing adapter protein; Toll like receptor adaptor protein; Wyatt.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg

抗體來(lái)源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Dog, Pig, Cow, Horse, Guinea Pig 

產(chǎn)品類型   一抗 磷酸化抗體  

研究領(lǐng)域    心 細(xì)胞生物 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo)

蛋白分子量  predicted molecular weight: 24kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated Synthesised acetylpeptide derived from human TIRAP around the acetylation site of Tyr86 [KD(p-Y)DV] 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

磷酸化白細(xì)胞介素1受體銜接蛋白抗體費(fèi)用 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The Toll-like receptor (TLR) family in mammals comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TIRAP (TIR domain-containing adaptor protein) is an adaptor protein used by TLR4. Blocking TIRAP inhibits TLR4-mediated signaling events, including DC maturation and cytokine production.

Function : Adapter involved in TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6.

Subunit : Homodimer. Also forms heterodimers with MyD88. Binds to TLR4 and IRAK2 via their respective TIR domains. Binds to BMX, PKR and TBK1. Does not interact with IRAK1, nor TLR9.

Subcellular Location : Cytoplasm.

Tissue Specificity : Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain.

Post-translational modifications : Phosphorylated by IRAK1 and IRAK4. Also phosphorylated by BTK.

Polyubiquitinated. Polyubiquitination follows phosphorylation by BTK and leads to TIRAP degradation.

Similarity : Contains 1 TIR domain.

Database links : UniProtKB/Swiss-Prot: P58753.2

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Rabbit anti-mouse IgG1, kappa chain 兔抗小鼠IgG1k鏈 (親和層析純化)Multi-class antibodies規(guī)格： 1mg

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FAM160B1 英文名稱： FAM160B1蛋白抗體 0.2ml

Rhesus antibody Rh Phospho-S6 Ribosomal Protein (Ser235+Ser236) 磷酸化S6核糖體蛋白抗體 規(guī)格 0.1ml

Rabbit anti-mouse IgG1, kappa chain 兔抗小鼠IgG1k鏈 (親和層析純化)Multi-class antibodies規(guī)格： 1mg

CD122/IL-2RB(Imterleukm-2 Receptor beta) CD122/白介素2受體β鏈抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-beta-Amyloid(1-42) β淀粉樣肽(1-42)抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh phospho-Bid (Ser65) 磷酸化BH3結(jié)構(gòu)域凋亡誘導(dǎo)蛋白抗體 規(guī)格 0.1ml

ELISA試劑盒開(kāi)發(fā)服務(wù)(10kit以內(nèi)) 次 西唐

VWF 英文名稱： 假性因子/性因子抗體 0.1ml

DPF2 英文名稱： 雙PHD-D4鋅指蛋白2抗體 0.2ml

Anti-beta-Amyloid(1-42) β淀粉樣肽(1-42)抗體Multi-class antibodies規(guī)格： 0.1ml

Anti- Beta-HCG/Gold 膠體金離子標(biāo)記小鼠抗人β亞基人絨毛膜激素單抗IgGMulti-class antibodies規(guī)格： 0.2ml

ECP(eosinophil cationic protein)mouse rat 嗜酸性粒細(xì)胞陽(yáng)離子蛋白抗原Multi-class antibodies規(guī)格： 0.5mg

芳香化酶抗體 Anti-Aromatase 0.1ml

ZNF473 英文名稱： 鋅指蛋白473抗體 0.2ml

DNA Ligase IV 英文名稱： DNA連接酶4抗體 0.2ml

Rhesus antibody Rh phospho-eIF4EBP1/2/3(Thr36) 磷酸化4E結(jié)合蛋白1,2,3抗體 規(guī)格 0.1ml

ECP(eosinophil cationic protein)mouse rat 嗜酸性粒細(xì)胞陽(yáng)離子蛋白抗原Multi-class antibodies規(guī)格： 0.5mg

人β細(xì)胞素(BTC) ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

兔子核因子-κB亞基p65親和肽(NF-κB p65)免疫試劑盒 Rabbit nuclear factor-κB p65,NF-κB p65 ELISA Kit

山梨醇脫氫酶(SDH)ELISA試劑盒 ，英文名： SDH ELISA Kit

Porcinelipoproteinα,Lp-αELISA試劑盒豬脂蛋白α(Lp-α)ELISA試劑盒規(guī)格：96T/48T

Humanalpha-fetoproteinLensculinarisaggliin3,AFP-L3ELISA試劑盒人小扁結(jié)合型甲胎蛋白/甲胎蛋白異質(zhì)體3(AFP-L3)ELISA試劑盒規(guī)格：96T/48T

HumanAprotinin，APELISAKit人抑肽酶(AP)ELISA試劑盒規(guī)格：96T/48T

大鼠瘦素受體(LEPR)ELISA試劑盒 ，英文名： LEPR ELISA Kit

Mouse vitamin C (VC) ELISA Kit 小鼠維生素C(VC)ELISA試劑盒

RatFMS-liketyrosinekinase3ligand,Flt-3LELISAKit 大鼠FMS樣酪氨酸激酶3配體(Flt3L)ELISA試劑盒 96T/48T 進(jìn)口分裝

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細(xì)胞SYK激酶活性定量檢測(cè)試劑盒(A/B/C)20次

ELISAKit1,3-βDglu大鼠1,3-βD葡葡糖苷酶規(guī)格：48T/96T

磷酸化白細(xì)胞介素1受體銜接蛋白抗體費(fèi)用 大鼠微粒體谷胱甘肽S轉(zhuǎn)移酶1(MGST1)ELISA試劑盒 ，英文名： MGST1 ELISA Kit

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CLIAKitforHpt/HP(MouseHaptoglobin)ELISAKit小鼠結(jié)合珠蛋白/觸珠蛋白規(guī)格：48T/96T

細(xì)胞PAK1激酶活性定量檢測(cè)試劑盒(A/B/C)20次

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抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 磷酸化白細(xì)胞介素1受體銜接蛋白抗體費(fèi)用 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；

3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）磷酸化白細(xì)胞介素1受體銜接蛋白抗體費(fèi)用 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。