產(chǎn)品訂購信息：
英文名稱  Anti-TROVE-2/SSA2 

中文名稱   相關(guān)蛋白2抗體說明書 

別    名  TROVE2; 60 kDa ribonucleoprotein Ro; 60 kDa Ro protein; 60 kDa SS A/Ro ribonucleoprotein; 60 kDa SS-A/Ro ribonucleoprotein; Autoantigen Ro/SSA, 60-KD; bA101E13.2; Gastric cancer multi drug resistance protein; Ribonucleoprotein autoantigen SS A/Ro; RO 60; Ro 60 kDa autoantigen; RO60; RO60_HUMAN; RoRNP; Sjoegren syndrome antigen A2; Sjoegren syndrome type A antigen; Sjogren syndrome antigen A2 (60kD ribonucleoprotein autoantigen SS A/Ro); Sjogren syndrome antigen A2 (60kDa ribonucleoprotein autoantigen SS A/Ro); Sjogren syndrome antigen A2; SS A; SS-A; SS-A/Ro; SSA 2; SSA; SSA2; TROVE 2; TROVE domain family member 2; TROVE2.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 免疫學(xué) 表觀遺傳學(xué)

蛋白分子量  predicted molecular weight: 61kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human TROVE-2/SSA2 (351-450aa) 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

相關(guān)蛋白2抗體說明書 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Ro autoantigens are of clinical significance because antibodies directed against them are found in most patients with primary Sjqgren syndrome, subacute cutaneous lupus erythematosus (SLE), neonatal lupus erythematosus, ANA-negative lupus erythematosus, and systemic lupus erythematosus-like disease secondary to homozygous C2 or C4 complement deficiency (1). Ro/SSA is a ribonucleoprotein that binds to autoantibodies in 35 to 50% of patients with SLE and in up to 97% of patients with Sjqgren syndrome (2). The Ro/SSA particle consists of a single immunoreactive protein noncovalently bound with one of four small RNA molecules (2). Most anti-Ro/SSA-positive sera antibodies detect not only the main protein, but also a smaller Ro/SSA protein (2). The genes which encode the smaller and larger proteins map to human chromosomes 11p15.5 and 1q31, respectively (3?). La/SSB is an autoimmune RNA-binding protein that plays a role in the transcription of RNA polymerase III was originally defined by its reactivity with autoantibodies from patients with Sj鰃ren syndrome and SLE (6).

Function : RNA-binding protein that binds to several small cytoplasmic RNA molecules known as Y RNAs. May stabilize these RNAs from degradation.

Subunit : Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Found in a complex with PUF60 and Y5 RNA. Interacts with RIP11.

Subcellular Location : Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR.

Similarity : Belongs to the Ro 60 kDa family. Contains 1 TROVE domain.

Database links : UniProtKB/Swiss-Prot: P10155.2

Anti-Phospho-mu Opioid Receptor (Ser375) /FITC 熒光素標(biāo)記磷酸化μ-型受體抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rabbit Anti-mouse IgM/HRP 辣根過氧化物酶標(biāo)記兔抗小鼠IgMMulti-class antibodies規(guī)格： 0.1ml

c-erbB-4癌基因抗體 Anti-C-erbB-4/HER4/erbB-4 0.1ml

Rabbit Anti-horse IgM/RBITC 羅丹明標(biāo)記的兔抗馬IgM 0.1ml

FGF14 英文名稱： 成纖維細(xì)胞生長因子14抗體 0.2ml

Rhesus antibody Rh PKC-γ/SCA14 蛋白激酶C亞型G抗體 規(guī)格 0.2ml

Rabbit Anti-mouse IgM/HRP 辣根過氧化物酶標(biāo)記兔抗小鼠IgMMulti-class antibodies規(guī)格： 0.1ml

HSP47(Heat shock protein-47) 熱休克蛋白-47抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-phospho-c-Abl(Tyr245) 磷酸化非受體酪氨酸激酶c-Abl抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh Phospho-CHEK1 (Ser296) 磷酸化細(xì)胞周期檢測點激酶1抗體 規(guī)格 0.1ml

兔抗人IgG-F(ab)2 0.5ml 國產(chǎn)

WD SOCS box protein 2 英文名稱： 信號傳導(dǎo)抑制蛋白WD重復(fù)蛋白2抗體 0.2ml

Phospho-Dab1 (Tyr220) 英文名稱： 磷酸化Disabled 1抗體 0.1ml

Anti-phospho-c-Abl(Tyr245) 磷酸化非受體酪氨酸激酶c-Abl抗體Multi-class antibodies規(guī)格： 0.1ml

Anti-AFP/Biotin 生物素化鼠抗人甲胎蛋白單克隆抗體(檢測)IgGMulti-class antibodies規(guī)格： 0.2ml

E2F1 轉(zhuǎn)錄因子E2F-1Multi-class antibodies規(guī)格： 0.5mg

磷酸化APP(Tyr757)淀粉樣肽前體蛋白抗體 Anti-phospho-APP 0.1ml

ZnT-1 英文名稱： 鋅轉(zhuǎn)運蛋白1抗體 0.2ml

DHRS2 英文名稱： 脫氫酶/還原酶家族成員2抗體 0.1ml

Rhesus antibody Rh Phospho-eNOS(Ser1177) 磷酸化合成酶3抗體(內(nèi)皮型) 規(guī)格 0.1ml

E2F1 轉(zhuǎn)錄因子E2F-1Multi-class antibodies規(guī)格： 0.5mg

人γ肽(Pγ)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

兔子基質(zhì)金屬蛋白酶抑制因子1(TIMP-1)免疫試劑盒 Rabbit tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA Kit

神經(jīng)酸酶(NA)ELISA試劑盒 ，英文名： NA ELISA Kit

Porcineierleukin4,IL-4ELISA試劑盒豬白介素4(IL-4)ELISA試劑盒規(guī)格：96T/48T

Humanaldehydedehydrogenase,ALDHELISA試劑盒人乙脫氫酶(ALDH)ELISA試劑盒規(guī)格：96T/48T

HumanApolipoproteinC3，ApoC3ELISAKit人載脂蛋白C3(ApoC3)ELISA試劑盒規(guī)格：96T/48T

大鼠水通道蛋白1(AQP-1)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

人鐵調(diào)素(Hepc)免疫試劑盒 Human hepcidin,Hepc ELISA Kit

中性木聚糖酶測試盒 可見分光光度法 50管/24樣

RatsolubleToll-likereceptor6,sTLR-6ELISA試劑盒大鼠可溶性Toll樣受體6(sTLR6)ELISA試劑盒規(guī)格：96T/48T

HumanKallikrein11,KLK11ELISA試劑盒人激肽釋放酶11(KLK11)ELISA試劑盒規(guī)格：96T/48T

HumanNein-1,n1ELISAKit人軸突生長誘向因子1(n1)ELISA試劑盒規(guī)格：96T/48T

相關(guān)蛋白2抗體說明書 大鼠維生素B6(VB6)ELISA試劑盒 ，英文名： VB6 ELISA Kit

人顆粒酶B(Gzms-B)ELISA檢測試劑盒HumangranzymesB,Gzms-BELISAKit 96T/48T

倉鼠脂氧素A4(LXA4)免疫試劑盒 Hamster Lipoxin A4,LXA4 ELISA Kit

CLIAKitforRBC(Humanai-redcellaibody)ELISAKit人抗紅細(xì)胞抗體規(guī)格：48T/96T

石蠟切片透明質(zhì)酸染色試劑盒50次

ELISAKitON人骨粘連蛋白規(guī)格：48T/96T

抗體的生物素化標(biāo)記實驗要點：

1. 相關(guān)蛋白2抗體說明書 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）相關(guān)蛋白2抗體說明書 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴(kuò)散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力。抗體的特異性高,它的識別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。