抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 突觸融合蛋白結(jié)合蛋白5抗體規(guī)格 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；

3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

產(chǎn)品訂購(gòu)信息：
英文名稱  Anti-Tomosyn/STXBP5 

中文名稱   突觸融合蛋白結(jié)合蛋白5抗體規(guī)格 

別    名  FLJ30922; Lethal(2) giant larvae protein homolog 3; LGL3; LLGL3; MGC141942; MGC141968; Nbla04300; STXBP5; Syntaxin binding protein 5; Syntaxin-binding protein 5; Tomosyn 1; STXB5_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來(lái)源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 神經(jīng)生物學(xué) 細(xì)胞凋亡 細(xì)胞周期蛋白 結(jié)合蛋白

蛋白分子量  predicted molecular weight: 127kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human Tomosyn/STXBP5 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

突觸融合蛋白結(jié)合蛋白5抗體規(guī)格 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 WD-repeats are motifs that are found in a variety of proteins and are characterized by a conserved core of 40-60 amino acids that commonly form a tertiary propeller structure. While proteins that contain WD-repeats participate in a wide range of cellular functions, they are generally involved in regulatory mechanisms concerning chromatin assembly, cell cycle control, signal transduction, RNA processing, apoptosis and vesicular trafficking. Tomosyn, also known as STXBP5 (syntaxin binding protein 5), LLGL3 or LGL3, is a 1,151 amino acid protein that localizes to the cytoplasm, as well as to the cell junction, secretory vesicles and to the peripheral membrane and contains one v-SNARE coiled-coil homology domain and 14 WD repeats. Interacting with Syntaxin 1 and Syntaxin 1B, Tomosyn functions as a regulator of neurotransmitter release and calcium-dependent exocytosis. Additionally, Tomosyn inhibits membrane fusion and may play a role in the assembly of SNARE complexes between transport vesicles and the plasma membrane. Multiple isoforms of Tomosyn exist due to alternative splicing events.

Function : Tomosyn plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release by inhibiting the assembly of trans-SNARE complexes between transport vesicles and the plasma membrane, via the displacement of munc18 from syntaxin-1. There are three splice variants of tomosyn designated m-tomosyn, b-tomosyn and s-tomosyn. Although b-tomosyn is ubiquitously expressed, s-tomosyn and m-tomosyn are expressed primarily in brain.

Subunit : Interacts with STX1A and STX1B via its v-SNARE homology domain. Part of a complex that contains STX1, STXBP5, SNAP25 and SYT1. Part of a complex that contains STXBP5, STX4A and SNAP23 (By similarity).

Subcellular Location : Cytoplasm (By similarity). Cell membrane; Peripheral membrane protein (By similarity). Cytoplasmic vesicle membrane; Peripheral membrane protein (By similarity). Cytoplasmic vesicle, secretory vesicle, synaptic vesicle (By similarity). Cell junction, synapse (By similarity). Note=Cytoplasmic, and associated with vesicular membranes and the plasma membrane. Detected at synapses and on synaptic vesicles (By similarity).

Similarity : Belongs to the WD repeat L(2)GL family.

Contains 1 v-SNARE coiled-coil homology domain.

Contains 14 WD repeats.

Database links : UniProtKB/Swiss-Prot: Q5T5C0.1

抗體的鑒定：

1）突觸融合蛋白結(jié)合蛋白5抗體規(guī)格 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。

Anti-PSCA /FITC 熒光素標(biāo)記抗干細(xì)胞抗原抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rabbit Anti-pig IgG Whole serum 兔抗豬IgG抗血清Multi-class antibodies規(guī)格： 1ml

生長(zhǎng)抑制因子基因1抗體 anti-ING1/p33 0.2ml

Rabbit anti-Bovine IgM whole serum 兔抗IgM抗血清 1ml

FAN1 英文名稱： 范可尼綜合征相關(guān)蛋白FAN1抗體 0.2ml

Rhesus antibody Rh phospho-PRKD1(Tyr463) 磷酸化蛋白激酶C mu型抗體 規(guī)格 0.1ml

Rabbit Anti-pig IgG Whole serum 兔抗豬IgG抗血清Multi-class antibodies規(guī)格： 1ml

BCRP/ABCG2(breast cancer resistance protein) 癌耐藥相關(guān)蛋白(抗原)Multi-class antibodies規(guī)格： 0.5mg

Anti-c-Abl 非受體酪氨酸激酶c-Abl抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh phospho-CHEK1(Ser317) 磷酸化細(xì)胞周期檢測(cè)點(diǎn)激酶1抗體 規(guī)格 0.1ml

兔抗Biotin (生物素)血清 0.5ml 國(guó)產(chǎn)

WDR16 英文名稱： WD重復(fù)蛋白16抗體 0.2ml

Phospho-Dab1 (Ser491) 英文名稱： 磷酸化Disabled 1抗體 0.1ml

Anti-c-Abl 非受體酪氨酸激酶c-Abl抗體Multi-class antibodies規(guī)格： 0.1ml

Anti-CD167a(DDR1)/HRP 辣根過(guò)氧化物酶標(biāo)記CD167a抗體IgGMulti-class antibodies規(guī)格： 0.2ml

ECE1(Endothelin Converting Enzyme 1) 內(nèi)皮素轉(zhuǎn)化酶1抗原Multi-class antibodies規(guī)格： 0.5mg

ADP核糖基化因子相互作用蛋白1抗體 Anti-Arfaptin 1 0.1ml

ZNF146 英文名稱： 鋅指蛋白146抗體 0.2ml

DHFR 英文名稱： 二氫葉酸還原酶抗體 0.1ml

Rhesus antibody Rh phospho-eIF4EBP1/4EBP1(Ser65/Thr70) 磷酸化eIF4E結(jié)合蛋白抗體 規(guī)格 0.1ml

ECE1(Endothelin Converting Enzyme 1) 內(nèi)皮素轉(zhuǎn)化酶1抗原Multi-class antibodies規(guī)格： 0.5mg

人橙(AO)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

兔子堿性成纖維細(xì)胞生長(zhǎng)因子(bFGF)免疫試劑盒 Rabbit basic fibroblast growth factor,bFGF ELISA Kit

神經(jīng)穿透素1(NPTX-1)ELISA試劑盒 ，英文名： NPTX-1 ELISA Kit

Porcineierleukin2,IL-2ELISA試劑盒豬白介素2(IL-2)ELISA試劑盒規(guī)格：96T/48T

HumanAlanineAminoansferase,ALT/GPTELISA試劑盒人氨酸轉(zhuǎn)氨酶/谷轉(zhuǎn)氨酶(ALT/GPT)ELISA試劑盒規(guī)格：96T/48T

Humanapelin13,AP13ELISAKit人apelin13(AP13)ELISA試劑盒規(guī)格：96T/48T

大鼠水通道蛋白2(AQP-2) ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

人鐵調(diào)素25(Hepc25)免疫試劑盒 Human Hepcidin 25,Hepc25 ELISA Kit

幾丁質(zhì)酶測(cè)試盒 可見分光光度法 50管/24樣

RatsolubleToll-likereceptor2,sTLR-2ELISA試劑盒大鼠可溶性Toll樣受體2(sTLR2)ELISA試劑盒規(guī)格：96T/48T

HumanKallikrein10,KLK10ELISA試劑盒人激肽釋放酶10(KLK10)ELISA試劑盒規(guī)格：96T/48T

HumanNervegrowthfactor,NGFELISAKit人的神經(jīng)生長(zhǎng)因子(NGF)ELISA試劑盒規(guī)格：96T/48T

突觸融合蛋白結(jié)合蛋白5抗體規(guī)格 大鼠維生素C(VC)ELISA試劑盒 ，英文名： VC ELISA Kit

人顆粒酶A(Gzms-A)ELISA檢測(cè)試劑盒HumangranzymesA,Gzms-AELISAKit 96T/48T

倉(cāng)鼠壞死因子α(TNF-α)免疫試劑盒 Hamster Tumor necrosis factor α,TNF-α ELISA kit

CLIAKitforRBC(Ratai-redcellaibody)ELISAKit大鼠抗紅細(xì)胞抗體規(guī)格：48T/96T

石蠟切片特恩布爾(TURNBULL-DAB)非血紅素鐵(二價(jià))強(qiáng)化染色試劑盒50次

ELISAKitoligomeric人寡聚蛋白規(guī)格：48T/96T