![]() |
產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
品牌 | 上海莼試 |
保存條件 | Store at -20 °C |
貨號(hào) | CS11744 |
應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 |
CAS編號(hào) | |
抗體名 | Anti-TEF3/TEAD4 |
克隆性 | |
靶點(diǎn) | 詳見說明書 |
適應(yīng)物種 | 詳見說明書 |
形態(tài) | 詳見說明書 |
宿主 | 詳見說明書 |
亞型 | IgG |
標(biāo)識(shí)物 | 詳見說明書 |
濃度 | 1mg/1ml% |
免疫原 | KLH conjugated synthetic peptide derived from human TEF3/TEAD4 |
抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn):
1. 轉(zhuǎn)錄增強(qiáng)因子TEF3抗體說明書 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析;
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件;
3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活;
4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等;
5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性;
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合;
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用;
8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。
產(chǎn)品訂購(gòu)信息:
英文名稱 Anti-TEF3/TEAD4
中文名稱 轉(zhuǎn)錄增強(qiáng)因子TEF3抗體說明書
別 名 EFTR 2; EFTR2; hRTEF 1B; hRTEF1B; MGC9014; Related to TEF 1; Related to TEF1; Related transcription enhancer factor 1B; RTEF1; TCF13L1; TEA domain family member 4; TEAD 4; TEAD-4; TEAD4; TEAD4_HUMAN; TEF 3; TEF-3; TEF3; TEFR 1; TEFR1; Transcription factor 13 (SV40 transcriptional enhancer factor) like 1; Transcription factor 13 like 1; Transcription factor 13-like 1; Transcription factor RTEF 1; Transcription factor RTEF-1; Transcription factor RTEF1; Transcriptional enhancer factor 1 related; Transcriptional enhancer factor 3; Transcriptional enhancer factor TEF 3; Transcriptional enhancer factor TEF-3; Transcriptional enhancer factor TEF3.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Zebrafish
產(chǎn)品類型 一抗
研究領(lǐng)域 心 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué)
蛋白分子量 predicted molecular weight: 48kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human TEF3/TEAD4
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
轉(zhuǎn)錄增強(qiáng)因子TEF3抗體說明書 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
(石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 TEF-3, is a 427 amino acid member of the transcriptional enhancer factor (TEF) family of proteins that are characterized by the presence of a TEA DNA-binding domain. Localized to the nucleus and expressed primarily in skeletal muscle, TEF-3 functions as a transcriptional regulator by binding specifically and non-cooperatively to the M-CAT motif found in the promotors of muscle-specific genes, thereby directing their subsequent expression. TEF-3 contains one TEA DNA-binding domain and is expressed as multiple isoforms due to alternative splicing events.
Function : ranscription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif.
Subunit : Interacts with YAP1 and WWTR1/TAZ.
Subcellular Location : Nucleus.
Tissue Specificity : Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.
Similarity : Contains 1 TEA DNA-binding domain.
Database links : UniProtKB/Swiss-Prot: Q15561.3
抗體的鑒定:
1)轉(zhuǎn)錄增強(qiáng)因子TEF3抗體說明書 抗體的效價(jià)鑒定:不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。
2)抗體的特異性鑒定:抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力??贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3)抗體親和力:是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol??贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。
Anti-PIRH2/FITC 熒光素標(biāo)記泛素連接酶抗體IgGMulti-class antibodies規(guī)格: 0.2ml
Rabbit Anti-rat IgG 兔抗大鼠IgG (親和層析純化)Multi-class antibodies規(guī)格: 1mg
糖原合酶激酶-3β抗體 Anti-GSK-3β 0.1ml
Dog IgM/Cy5 Cy5標(biāo)記的兔抗犬IgM抗體 0.1ml
FAM59A 英文名稱: FAM59A蛋白抗體 0.2ml
Rhesus antibody Rh phospho-RUNX3(Ser338) 磷酸化Runx3抗體 規(guī)格 0.1ml
Rabbit Anti-rat IgG 兔抗大鼠IgG (親和層析純化)Multi-class antibodies規(guī)格: 1mg
Hpt/HP(haptoglobin) 結(jié)合珠蛋白/觸珠蛋白抗原Multi-class antibodies規(guī)格: 0.5mg
Anti-ABCG1 三磷酸腺苷結(jié)合盒亞家族G1抗體Multi-class antibodies規(guī)格: 0.2ml
Rhesus antibody Rh Phospho-CHK2 (ser516) 磷酸化細(xì)胞周期檢測(cè)點(diǎn)激酶2抗體 規(guī)格 0.1ml
兔抗小鼠IgG~Fc 0.5ml 國(guó)產(chǎn)
WIF1 英文名稱: Wnt信號(hào)抑制因子1抗體 0.2ml
Phospho-DOK2 (Tyr351) 英文名稱: 磷酸化D酪酸激酶衰減蛋白2抗體 0.1ml
Anti-ABCG1 三磷酸腺苷結(jié)合盒亞家族G1抗體Multi-class antibodies規(guī)格: 0.2ml
Anti-CD86/B7-2/RBITC 紅色羅丹明標(biāo)記CD86蛋白抗體抗體IgGMulti-class antibodies規(guī)格: 0.2ml
CRF (Corticotropin-Releasing Factor) 促腎上腺皮質(zhì)激素釋放因子Multi-class antibodies規(guī)格: 0.5mg
整合素樣金屬蛋白酶與凝血酶1型-7(抗體) Anti-ADAM-TS7 0.1ml
ZNF434 英文名稱: 蛋白5/鋅指蛋白434抗體 0.2ml
Defensin alpha 5 英文名稱: 防御素α5抗體 0.2ml
Rhesus antibody Rh phospho-FAS (Tyr291) 磷酸化載脂蛋白A1抗體 規(guī)格 0.1ml
CRF (Corticotropin-Releasing Factor) 促腎上腺皮質(zhì)激素釋放因子Multi-class antibodies規(guī)格: 0.5mg
人癌易感蛋白2(BRCA-2)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝
小鼠樣生長(zhǎng)因子結(jié)合蛋白3(IGFBP-3)免疫試劑盒 Mouse Insulin-like growth factor binding protein 3,IGFBP-3 ELISA KIT
炭疽桿菌(BA)核酸檢測(cè)試劑盒(PCR-熒光探針法) 48T
HumanProstaticAcidPhosphatase,PAPELISA試劑盒人酸性磷酸酶(PAP)ELISA試劑盒規(guī)格:96T/48T
ELISAKitCA724小鼠標(biāo)志物規(guī)格:48T/96T
HumanComplemefragme3b,C3bELISAKit人補(bǔ)體片斷3b(C3b)ELISA試劑盒規(guī)格:96T/48T
大鼠絲蛋白結(jié)合LIM蛋白1(FBLIM1)ELISA試劑盒 ,英文名: FBLIM1 ELISA Kit
Mouse vitamin A (VA) ELISA Kit 小鼠維生素A(VA)ELISA試劑盒
RatProgesterone,PROGELISAkit 大鼠孕激素/孕酮(PROG)ELISA試劑盒 96T/48T 進(jìn)口分裝
CLIAKitforHGH(Humangrowthhormone)ELISAKIT人生長(zhǎng)激素規(guī)格:48T/96T
細(xì)胞SPHK1激酶活性定量檢測(cè)試劑盒(A/B/C)20次
ELISAKiX大鼠Ⅰ型膠原N末端肽規(guī)格:48T/96T
轉(zhuǎn)錄增強(qiáng)因子TEF3抗體說明書 大鼠維生素E(VE)ELISA試劑盒 ,英文名: VE ELISA Kit
人Ⅲ型膠原(ColⅢ)ELISA檢測(cè)試劑盒HumanCollageypeⅢ,ColⅢELISAKIT 96T/48T
大鼠1,25二羥基維生素D3(1,25 DHVD3)免疫試劑盒 Rat 1,25-dihydroxyvitamin D3 ELISA Kit
CLIAKitforRBP-4(MouseRetinol-bindingprotein4)ELISAKit小鼠視黃醇結(jié)合蛋白4規(guī)格:48T/96T
石蠟切片蘇木素伊紅染色試劑盒50次
ELISAKitFDA人果糖1,6二磷酸縮酶規(guī)格:48T/96T