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產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
品牌 | 上海莼試 |
保存條件 | Store at -20 °C |
貨號(hào) | CS11738 |
應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 |
CAS編號(hào) | |
抗體名 | Anti-TSHZ3/ZNF537 |
克隆性 | |
靶點(diǎn) | 詳見說明書 |
適應(yīng)物種 | 詳見說明書 |
形態(tài) | 詳見說明書 |
宿主 | 詳見說明書 |
亞型 | IgG |
標(biāo)識(shí)物 | 詳見說明書 |
濃度 | 1mg/1ml% |
免疫原 | KLH conjugated synthetic peptide derived from human TSHZ3/ZNF537 |
產(chǎn)品訂購(gòu)信息:
英文名稱 Anti-TSHZ3/ZNF537
中文名稱 鋅指蛋白537抗體規(guī)格
別 名 teashirt homolog 3; Zinc finger protein 537; TSHZ3; TSH3; ZNF537.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit
產(chǎn)品類型 一抗
研究領(lǐng)域 細(xì)胞生物 發(fā)育生物學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué)
蛋白分子量 predicted molecular weight: 119kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human TSHZ3/ZNF537
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
鋅指蛋白537抗體規(guī)格 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200
(石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 The homeobox DNA-binding domain is a 60 amino acid motif that is conserved among many species and functions to bind DNA via a helix-turn-helix structure, thereby playing a role in transcriptional regulation and the control of gene expression. TSHZ3 (teashirt zinc finger homeobox 3), also known as KIAA1474, TSH3 or ZNF537, is a 1,081 amino acid protein that contains one homeobox DNA-binding domain and five C2H2-type zinc fingers. Localized to the nucleus, TSHZ3 functions as a transcriptional regulator that is involved in developmental processes throughout the body. The gene encoding TSHZ3 maps to human chromosome 19, which consists of over 63 million bases, houses approximately 1,400 genes and is recognized for having the greatest gene density of the human chromosomes.
Function : Transcriptional regulator involved in developmental processes. Function in association with APBB1, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. TSHZ3-mediated transcription repression involves the recruitment of histone deacetylases HDAC1 and HDAC2. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s). Regulates the development of neurons involved in both respiratory rhythm and airflow control. Promotes maintenance of nucleus ambiguus (nA) motoneurons, which govern upper airway function, and establishes a respiratory rhythm generator (RRG) activity compatible with survival at birth. Involved in the differentiation of the proximal uretic smooth muscle cells during developmental processes. Involved in the up-regulation of myocardin, that directs the expression of smooth muscle cells in the proximal ureter.
Subunit : Interacts (via homeobox domain) with APBB1 (via PID domain 1). Interacts (via N-terminus) with HDAC1 and HDAC2; the interaction is direct. Found in a trimeric complex with APBB1 and HDAC1; the interaction between HDAC1 and APBB1 is mediated by TSHZ3.
Subcellular Location : Nucleus. Cell projection, growth cone. Note=Colocalizes with APBB1 in axonal grwoth cone. Colocalizes with APBB1 in the nucleus.
Tissue Specificity : Expressed in brain; strongly reduced in post-mortem elderly subjects with Alzheimer disease.
Similarity : Belongs to the teashirt C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers.
Contains 1 homeobox DNA-binding domain.
Anti-SIAH1/FITC 熒光素標(biāo)記泛素連接酶Siah1抗體IgGMulti-class antibodies規(guī)格: 0.2ml
Rabbit Anti-rat IgM 兔抗大鼠IgM (親和層析純化)Multi-class antibodies規(guī)格: 1mg
三磷酸鳥苷環(huán)水解酶抗體 Anti-GTP-CH-1 0.2ml
Dog IgM/Cy3 Cy3標(biāo)記的兔抗犬IgM抗體 0.1ml
FAM59B 英文名稱: FAM59B蛋白抗體 0.2ml
Rhesus antibody Rh phospho-RUNX2(Ser451) 磷酸化成骨特異性轉(zhuǎn)錄因子抗體 規(guī)格 0.1ml
Rabbit Anti-rat IgM 兔抗大鼠IgM (親和層析純化)Multi-class antibodies規(guī)格: 1mg
phospho-HP1/heterochromatin protein 1 (pTyr24)(fruit fly) 異染色質(zhì)蛋白1(磷酸化多肽)Multi-class antibodies規(guī)格: 0.5mg
Anti-ABCA1 腺苷三磷酸結(jié)合盒轉(zhuǎn)運(yùn)體A1抗體Multi-class antibodies規(guī)格: 0.1ml
Rhesus antibody Rh phospho-c-Jun(Ser63) 磷酸化原癌基因c-Jun抗體 規(guī)格 0.1ml
兔抗狗IgG血清 0.5ml 國(guó)產(chǎn)
WISP39 英文名稱: p21調(diào)控蛋白WISP39抗體 0.2ml
DRD4 英文名稱: 多巴胺受體D4抗體 0.1ml
Anti-ABCA1 腺苷三磷酸結(jié)合盒轉(zhuǎn)運(yùn)體A1抗體Multi-class antibodies規(guī)格: 0.1ml
Anti-XIAP/FITC 熒光素標(biāo)記X-連鎖凋亡蛋白/性連鎖凋亡抑制蛋白抗體IgGMulti-class antibodies規(guī)格: 0.2ml
CX36 (Connexin-36) 間隙連接蛋白36抗原Multi-class antibodies規(guī)格: 0.5mg
肝再生增強(qiáng)因子抗體 Anti-ALR 0.1ml
ZNF717 英文名稱: 鋅指蛋白717抗體 0.2ml
DBX1 英文名稱: 腦發(fā)育同源蛋白1抗體 0.2ml
Rhesus antibody Rh Phospho-FAK(Tyr407) 磷酸化粘著斑激酶抗體 規(guī)格 0.1ml
CX36 (Connexin-36) 間隙連接蛋白36抗原Multi-class antibodies規(guī)格: 0.5mg
人白C 抗原(DR15)ELISA試劑盒 96T/48T 試劑盒 組裝/原裝
兔子可溶性CD40配體(sCD40L)免疫試劑盒 Rabbit soluble cluster of differeiation 40 ligand,sCD40L ELISA Kit
神經(jīng)特異性烯醇化酶(NSE)ELISA試劑盒 ,英文名: NSE ELISA Kit
Porcineiercellularadhesionmolecule2,ICAM-2ELISA試劑盒豬細(xì)胞間粘附分子2(ICAM-2/CD102)ELISA試劑盒規(guī)格:96T/48T
Humanadenylylcyclase1,AC-1ELISA試劑盒人腺苷酸環(huán)化酶1(AC-1)ELISA試劑盒規(guī)格:96T/48T
HumanAi-ThrombinⅢ,AT-ⅢELISAKit人抗凝血酶Ⅲ抗體(AT-Ⅲ)ELISA試劑盒規(guī)格:96T/48T
大鼠松弛肽/松弛素(RLN)ELISA試劑盒 ,英文名: RLN ELISA Kit
人組織蛋白去乙?;?HD)ELISA檢測(cè)試劑盒HumanHistoneDeacetylase,HDELISAKit 96T/48T
類人猿降鈣素(CT)免疫試劑盒 Simian Calcitonin,CT ELISA Kit
CLIAKitforP-PKCELISAKit大鼠磷酸化蛋白激酶C規(guī)格:48T/96T
石蠟切片組織FSH-BETA蛋白表達(dá)熒光顯微鏡檢測(cè)試劑盒10/20次
ELISAKitSIgA人分泌型免疫球蛋白A規(guī)格:48T/96T
鋅指蛋白537抗體規(guī)格 大鼠尾加壓素II(Urotensin II) ELISA試劑盒 96T/48T ELISA試劑盒 組裝/原裝
人維生素H(VH)免疫試劑盒 Human Vitamin H,VH ELISA Kit
脂肪酸合成酶(FAS)測(cè)試盒 紫外分光光度法 25管/24樣
RatProgesteronereceptor,PROGRELISA試劑盒小鼠孕同受體(PROGR)ELISA試劑盒規(guī)格:96T/48T
Humaniercellularadhesionmolecule2,ICAM-2ELISA試劑盒人細(xì)胞間粘附分子2(ICAM-2/CD102)ELISA試劑盒規(guī)格:96T/48T
humanmitogenactivatedproteinkinasekinase6,MKK6ELISAKit人原活化蛋白激酶激酶6(MKK6)ELISA試劑盒規(guī)格:96T/48T
抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn):
1. 鋅指蛋白537抗體規(guī)格 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析;
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件;
3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活;
4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等;
5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性;
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合;
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用;
8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。
抗體的鑒定:
1)鋅指蛋白537抗體規(guī)格 抗體的效價(jià)鑒定:不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。
2)抗體的特異性鑒定:抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力??贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3)抗體親和力:是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol??贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。