上海莼試生物技術(shù)有限公司
   
菜單 Close 公司首頁 公司介紹 公司動(dòng)態(tài) 產(chǎn)品展廳 證書榮譽(yù) 聯(lián)系方式 在線留言
您當(dāng)前的位置: 網(wǎng)站首頁 > 產(chǎn)品展廳 >抗體 >脂多糖相關(guān)反應(yīng)蛋白5/γ-taxilin抗體品牌
產(chǎn)品展廳
脂多糖相關(guān)反應(yīng)蛋白5/γ-taxilin抗體品牌
  • 品牌:上海莼試
  • 產(chǎn)地:進(jìn)口、國(guó)產(chǎn)
  • 貨號(hào):CS11735
  • 發(fā)布日期: 2019-01-23
  • 更新日期: 2025-03-17
產(chǎn)品詳請(qǐng)
產(chǎn)地 進(jìn)口、國(guó)產(chǎn)
品牌 上海莼試
保存條件 Store at -20 °C
貨號(hào) CS11735
應(yīng)用范圍 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200
CAS編號(hào)
抗體名 Anti-Gamma-taxilin/LSR5
克隆性
靶點(diǎn) 詳見說明書
適應(yīng)物種 詳見說明書
形態(tài) 詳見說明書
宿主 詳見說明書
亞型 IgG
標(biāo)識(shí)物 詳見說明書
濃度 1mg/1ml%
免疫原 KLH conjugated synthetic peptide derived from human Gamma-taxilin

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn):

1. 脂多糖相關(guān)反應(yīng)蛋白5/γ-taxilin抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析;

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件;

3.NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活;

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等;

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性;

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合;

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用;

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

產(chǎn)品訂購信息:
英文名稱  Anti-Gamma-taxilin/LSR5 

中文名稱   脂多糖相關(guān)反應(yīng)蛋白5/γ-taxilin抗體品牌 

      chromosome X open reading frame 15; CXorf15; Environmental lipopolysaccharide-responding gene protein; factor inhibiting activating transcription factor 4 (ATF4)-mediated transcription; Factor inhibiting ATF4-mediated transcription antibody FIAT; Gamma-taxilin; Lipopolysaccharide-specific response protein 5; LSR5; TXLNG; TXLNG_HUMAN.



       1mg/1ml

規(guī)   0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 干細(xì)胞 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué)

蛋白分子量  predicted molecular weight: 60kDa

       Lyophilized or Liquid

 KLH conjugated synthetic peptide derived from human Gamma-taxilin 

       IgG

純化方法   affinity purified by Protein A

儲(chǔ)    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

脂多糖相關(guān)反應(yīng)蛋白5/γ-taxilin抗體品牌 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

(石蠟切片需做抗原修復(fù)) 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 This gene encodes a member of the taxilin family. The encoded protein binds to the C-terminal coiled-coil region of syntaxin family members 1A, 3A and 4A, and may play a role in intracellular vesicle trafficking. This gene is up-regulated by lipopolysaccharide and the gene product may be involved in cell cycle regulation. The related mouse protein was also shown to inhibit activating transcription factor 4-mediated transcription and thus regulate bone mass accrual. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009].

Function : May be involved in intracellular vesicle traffic. Inhibits ATF4-mediated transcription, possibly by dimerizing with ATF4 to form inactive dimers that cannot bind DNA. May be involved in regulating bone mass density through an ATF4-dependent pathway. May be involved in cell cycle progression.

Subunit : Binds to the C-terminal coiled coil region of syntaxin family members STX1A, STX3A and STX4A. Forms a heterodimer with ATF4 in osteoblasts.

Subcellular Location : Nucleus membrane. Cytoplasm, cytosol.

Tissue Specificity : Ubiquitously expressed. Expressed at high level in heart and skeletal muscle. Expressed in brain, placenta, lung, liver, kidney and pancreas.

Similarity : Belongs to the taxilin family.

Database links : UniProtKB/Swiss-Prot: Q9NUQ3.2

抗體的鑒定:

1脂多糖相關(guān)反應(yīng)蛋白5/γ-taxilin抗體品牌 抗體的效價(jià)鑒定:不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2)抗體的特異性鑒定:抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力??贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3)抗體親和力:是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol??贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。

Anti-OPN/FITC 熒光素標(biāo)記骨橋蛋白抗體IgGMulti-class antibodies規(guī)格: 0.2ml

Rabbit Anti-rat IgM/HRP 辣根過氧化物酶標(biāo)記兔抗大鼠IgMMulti-class antibodies規(guī)格: 0.1ml

嗜鉻粒素B抗體 Anti-CGB 0.2ml

Rabbit Anti-horse IgM/APC APC標(biāo)記的兔抗馬IgM 0.1ml

FAM135B 英文名稱: FAM135B蛋白抗體 0.2ml

Rhesus antibody Rh PKC alpha 蛋白激酶C α抗體 規(guī)格 0.2ml

Rabbit Anti-rat IgM/HRP 辣根過氧化物酶標(biāo)記兔抗大鼠IgMMulti-class antibodies規(guī)格: 0.1ml

Anti-HMGA1 (high mobility group AT-hook 1) 高遷移率族蛋白A1抗原Multi-class antibodies規(guī)格: 0.5mg

Anti-AAK1 AP2關(guān)聯(lián)激酶1抗體Multi-class antibodies規(guī)格: 0.2ml

Rhesus antibody Rh phospho-c-Jun(Thr91+Thr93) 磷酸化原癌基因c-Jun抗體 規(guī)格 0.1ml

羊抗人IgG血清 0.5ml 國(guó)產(chǎn)

WNK4 英文名稱: WNK4抗體 0.1ml

DRD3 英文名稱: 多巴胺受體D3抗體 0.1ml

Anti-AAK1 AP2關(guān)聯(lián)激酶1抗體Multi-class antibodies規(guī)格: 0.2ml

Anti-CD8/PE 熒光素藻紅蛋白標(biāo)記兔抗人、大、小鼠CD8抗體IgGMulti-class antibodies規(guī)格: 0.2ml

EGF(Epidermal growth factor)rat 重組表皮生長(zhǎng)因子抗原(大鼠)Multi-class antibodies規(guī)格: 0.5mg

Artemin抗體 Anti-ART 0.1ml

ZBTB25 英文名稱: 鋅指蛋白46抗體 0.2ml

DAP1 英文名稱: 死亡相關(guān)蛋白1抗體 0.2ml

Rhesus antibody Rh phospho-EIF4EBP1(Ser111) 磷酸化eIF4E結(jié)合蛋白抗體 規(guī)格 0.1ml

EGF(Epidermal growth factor)rat 重組表皮生長(zhǎng)因子抗原(大鼠)Multi-class antibodies規(guī)格: 0.5mg

人白介素10(IL-10)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

兔子可溶性內(nèi)皮細(xì)胞蛋白C受體(sEPCR)免疫試劑盒 Rabbit soluble endothelial protein C receptor,sEPCR ELISA Kit

神經(jīng)調(diào)節(jié)蛋白2(G2)ELISA試劑盒 ,英文名: G2 ELISA Kit

Porcineinsulin-likegrowthfactors2,IGF-2ELISA試劑盒豬樣生長(zhǎng)因子2(IGF-2)ELISA試劑盒規(guī)格:96T/48T

HumanAdenovirusAigen,ADV-AgELISA試劑盒人腺病毒抗原(ADV-Ag)ELISA試劑盒規(guī)格:96T/48T

Humanai-septolysinO,ASOELISAKit人抗鏈球菌溶血素O/抗O(ASO)ELISA試劑盒規(guī)格:96T/48T

大鼠素受體2(C2)ELISA試劑盒 ,英文名: C2 ELISA Kit

Rat ierleukin 8 (IL-8/CXCL8) ELISA Kit 大鼠白介素8(IL-8/CXCL8)ELISA試劑盒

Ratiercellularadhesionmolecule2,ICAM-2ELISAKit 大鼠細(xì)胞間粘附分子2(ICAM-2/CD102)ELISA試劑盒 96T/48T 進(jìn)口分裝

CLIAKitforcTn-I(MousecardiacoponinI)ELISAKit小鼠心肌肌鈣蛋白Ⅰ規(guī)格:48T/96T

細(xì)胞糖原高酸希爾(PAS)法染色試劑盒50次

RatEotaxin1ELISAKit大鼠嗜酸粒細(xì)胞趨化蛋白Eotaxin1(Eotaxin1/CCL11)ELISA試劑盒規(guī)格:96T/48T

脂多糖相關(guān)反應(yīng)蛋白5/γ-taxilin抗體品牌 大鼠胃蛋白酶(PG)ELISA試劑盒 ,英文名: PG ELISA Kit

人表面抗原(HBsAg)ELISA檢測(cè)試劑盒HumanhepatitisBvirussurfaceaigen,HBsAgELISAKit 96T/48T

大鼠1,3-βD葡葡糖苷酶(1,3-βD glucosidase)免疫試劑盒 Rat 1,3-βD glucosidase ELISA Kit

CLIAKitforREG-4(HumaegeneratinggeneIV)ELISAKit人再生基因蛋白IV規(guī)格:48T/96T

石蠟切片神經(jīng)組織固藍(lán)(FASTBLUE)染色試劑盒50次

ELISAKitPPAR-α人過氧化物酶體增殖物激活受體α規(guī)格:48T/96T

 


聯(lián)系方式
手機(jī):13585831301
Q Q:
亚洲精品乱码久久久久久久久久久久,国产精品视频色尤物yw,亚洲AV日韩AV永久无码色欲,亚洲a∨精品永久无码