易感基因101蛋白抗體費用 英文名稱  Anti-Tsg101

中文名稱  易感基因101蛋白抗體費用 

別    名 ESCRT I complex subunit TSG101; ESCRT-I complex subunit TSG101; Tsg101; TSG 10; TSG 101; TSG10; Tumor susceptibility gene 10; Tumor susceptibility gene 101; Tumor susceptibility gene 101 protein; Tumor susceptibility protein; Tumor susceptibility protein isoform 3; VPS 23; VPS23; TS101_HUMAN.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應   Human, Mouse, Rat, Dog, Cow, Horse, Rabbit

產(chǎn)品類型   一抗  

研究領域     細胞生物 免疫學 染色質(zhì)和核信號 微生物學 細胞分化 表觀遺傳學

蛋白分子量  predicted molecular weight: 44kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human Tsg101 N-terminus

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

易感基因101蛋白抗體費用 產(chǎn)品應用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 

The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. TSG101 also interacts with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses like -1 p6, human spumavirus Gag, HTLV-1 Gag, as well as of Ebolavirus VP40.

Function : Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.

Subunit : Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, a VPS37 protein (VPS37A to -D) and a FAM125/MVB12 protein (FAM12* or -B) in a 1:1:1:1 stoechiometry. Interacts with VPS37A, VPS37B and VPS37C. Interacts with ubiquitin, stathmin, GMCL, DMAP1 and AATF (By similarity). Interacts with HGS; the interaction mediates the association with the ESCRT-0 complex. Interacts with GGA1 and GGA3. Interacts (via UEV domain) with PDCD6IP/AIP1. Interacts with VPS28, SNF8 and VPS36. Self-associates. Interacts with FAM12*/MVB12A; the association appears to be mediated by the TSG101-VPS37 binary subcomplex. Interacts with VPS37D. Interacts with LRSAM1. Interacts with CEP55; the interaction is required for cytokinesis but not for viral budding. Interacts with PDCD6. Interacts with HIV-1 p6. Interacts with human spumavirus Gag. Interacts with HTLV-1 Gag. Interacts with Ebola virus VP40. Interacts with EIAV p9; the interaction has been shown in vitro. Interacts with MGRN1.

Subcellular Location : Cytoplasm. Membrane; Peripheral membrane protein. Nucleus. Late endosome membrane; Peripheral membrane protein. Note=Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis.

Tissue Specificity : Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.

Post-translational modifications : Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.

Similarity : Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.

Contains 1 SB (steadiness box) domain.

Contains 1 UEV (ubiquitin E2 variant) domain.

Database links : UniProtKB/Swiss-Prot: Q99816.2

實驗流程：

全、新、優(yōu)、品、好四大特點：

易感基因101蛋白抗體費用 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標準品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

好：我公司具有優(yōu)質(zhì)的技術團隊，產(chǎn)品一旦售出，實驗過程中遇到困難可提供在線技術咨詢。使您使用產(chǎn)品時沒有任何的后顧之憂。

正在熱銷的相關產(chǎn)品：

Anti-PAFR/FITC 熒光素標記抗血小板活化因子受體抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rabbit IgG/Biotin 生物素化兔IgGMulti-class antibodies規(guī)格： 1ml

周期素D2抗體 Anti-Cyclin D2 0.1ml

Rabbit anti-mouse Kappa light chain/HRP 辣根過氧化物酶標記的兔抗小鼠k鏈 0.1ml

FXYD1 英文名稱： 磷酸神經(jīng)膜抗體 0.1ml

Rhesus antibody Rh phospho-VAV1(Tyr160) 磷酸化鳥苷酸轉(zhuǎn)換因子VAV1抗體 規(guī)格 0.1ml

Rabbit IgG/Biotin 生物素化兔IgGMulti-class antibodies規(guī)格： 1ml

Inhibin Alpha 抑制素α抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-AEV(Avian Encephalomyelitis virus) 禽腦脊髓炎病毒AEV抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh phospho-Cdc25B (Ser149) 磷酸化細胞分裂周期蛋白25B抗體 規(guī)格 0.1ml

溴化已錠染色液 -紅色 1ml Sigma原料,自產(chǎn)

Wnt1 英文名稱： 信號通路Wnt1抗體 0.1ml

Dynorphin A 英文名稱： 腦啡肽A抗體 0.2ml

Anti-AEV(Avian Encephalomyelitis virus) 禽腦脊髓炎病毒AEV抗體Multi-class antibodies規(guī)格： 0.2ml

Anti-HBeAg/FITC 熒光素標記小鼠抗人e抗原抗體(1)IgGMulti-class antibodies規(guī)格： 0.2ml

Des(Desmin) 結蛋白抗原Multi-class antibodies規(guī)格： 0.5mg

活化蛋白C抗體 Anti-APC 0.1ml

ZNF749 英文名稱： 鋅指蛋白749抗體 0.2ml

DAGLA 英文名稱： 神經(jīng)干細胞樹突調(diào)節(jié)蛋白DAGLα抗體 0.2ml

Rhesus antibody Rh phospho-Ephrin B(Ty*4/329) 磷酸化Ephrin B抗體 規(guī)格 0.1ml

Des(Desmin) 結蛋白抗原Multi-class antibodies規(guī)格： 0.5mg

人白介素11(IL-11)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

兔子可溶性粘附分子(Sam)免疫試劑盒 Rabbit soluble adhesion molecules,Sam ELISA Kit

神經(jīng)調(diào)節(jié)蛋白4(G4)ELISA試劑盒 ，英文名： G4 ELISA Kit

PorcineInfluenzavirusesA,FLUAELISA試劑盒豬病毒A(FLUA)ELISA試劑盒規(guī)格：96T/48T

Humanadenosinedeaminase,ADAELISA試劑盒人腺苷脫氨酶(ADA)ELISA試劑盒規(guī)格：96T/48T

HumanAi-SomaticMuscleAibody,ASAELISAKit人抗骨骼肌抗體(ASA)ELISA試劑盒規(guī)格：96T/48T

大鼠速激肽受體2(TACR2)ELISA試劑盒 ，英文名： TACR2 ELISA Kit

Mice of homocysteine (Hcy) ELISA Kit 小鼠同型半胱氨酸(Hcy)ELISA試劑盒

ratCoicosterone,COELISAKit 大鼠皮質(zhì)酮/腎上腺酮(CO)ELISA試劑盒 96T/48T 進口分裝

CLIAKitforHIS(MouseHistamine)ELISAKit小鼠組胺規(guī)格：48T/96T

細胞ROCK激酶總活性定量檢測試劑盒(A/B/C)20次

ELISAKitPⅢ大鼠Ⅲ型前膠原氨基端肽規(guī)格：48T/96T

易感基因101蛋白抗體費用 大鼠胃蛋白酶原A(PGA)ELISA試劑盒 ，英文名： PGA ELISA Kit

Bovine C reactive protein (CRP) ELISA Kit 牛C反應蛋白(CRP)ELISA試劑盒

MouseSolubleEndoglin,ENG/sCD105ELISAKit 小鼠可溶性Endoglin(ENG/sCD105)ELISA試劑盒 96T/48T 進口分裝

CLIAKitforhs-CRP(RabbithighsensitivityC-ReactiveProtein)ELISAKit兔超敏C反應蛋白規(guī)格：48T/96T

細胞NEK6激酶活性定量檢測試劑盒(A/B/C)20次

ELISAKitβ-EPR大鼠β內(nèi)啡肽受體規(guī)格：48T/96T

技術外包服務:

★易感基因101蛋白抗體費用 分子生物學：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學：基因合成、引物合成、基因測序、載體構建等

★蛋白工程：原核、哺乳動物蛋白表達系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細胞工程：細胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復、克隆形成)、細胞培養(yǎng)、細胞膜制備、穩(wěn)定細胞株構建、細胞RNAi技術等等。