抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 端粒調(diào)控因子抗體費(fèi)用 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；

3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

產(chǎn)品訂購(gòu)信息：
英文名稱  Anti-Tankyrase 

中文名稱   端粒調(diào)控因子抗體費(fèi)用 

別    名  PARP 5a; PARP5A; PARPL; pART5; Poly [ADP-ribose] polymerase 5A; TANK 1; TANK1; Tankyrase 1; Tankyrase I; Tankyrase TRF1 interacting ankyrin related ADP ribose polymerase; Tankyrase-1; Tankyrase1; TankyraseI; TIN 1; TIN1; TINF 1; TINF1; TNKS 1; TNKS; TNKS-1; TNKS1; TNKS1_HUMAN; TRF1 interacting ankyrin related ADP ribose polymerase; TRF1-interacting ankyrin-related ADP-ribose polymerase.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來(lái)源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Cow 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 免疫學(xué) 表觀遺傳學(xué)

蛋白分子量  predicted molecular weight: 146kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human Tankyrase 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

端粒調(diào)控因子抗體費(fèi)用 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Tankyrase-1 and the closely related homolog Tankyrase-2 arepoly(ADP-ribose) polymerases (PARPs) that co-localize and use an ankyrin-repeat domain to bind diverse proteins, including TRF-1 (telomere-repeat-binding factor 1), IRAP (insulin-responsive aminopeptidase) and TAB182. Tankyrase-1 (also known as TNKS and TNKS1) and Tankyrase-2 (also known as TNKS2, TNKL and TANK2) interact with the same set of proteins and probably mediate overlapping functions, both at telomeres and in vesicular compartments. Overexpression of Tankyrase-1 in the nucleus promotes telomere elongation, suggesting that Tankyrase 1 may regulate access of telomerase to the telomeric complex. Overexpression of Tankyrase-2 in the nucleus releases endogenous TRF1 from telomeres, establishing Tankyrase-2 as a PARP with itself and TRF1 as acceptors of ADP-ribosylation, and suggesting the possibility of a role for Tankyrase-2 at telomeres. The ankyrin (ANK) domain of Tankyrase-2 comprises five subdomains that provide redundant binding sites for IRAP. Tankyrase-2 lacks the N-terminal Histidine/Proline/Serine-rich region of Tankyrase-1, but contains a corresponding ankyrin repeat region, sterile ?motif module and poly(ADP-ribose) polymerase homology domain. The gene encoding Tankyrase-2 is widely expressed, with mRNA transcripts particularly abundant in skeletal muscle and placenta.

Subunit : Oligomerizes and associates with TNKS2. Interacts with the cytoplasmic domain of LNPEP/Otase in SLC2A4/GLUT4-vesicles. Binds to the N-terminus of telomeric TERF1 via the ANK repeats. Found in a complex with POT1; TERF1 and TINF2. Interacts with AXIN1, AXIN2, BLZF1 and CASC3.

Subcellular Location : Cytoplasm. Golgi apparatus membrane. Chromosome > centromere. Nucleus > nuclear pore complex. Chromosome > telomere. Associated with the Golgi and with juxtanuclear SLC2A4/GLUT4-vesicles. A minor proportion is also found at nuclear pore complexes and around the pericentriolar matrix of mitotic centromeres. During interphase, a small fraction of TNKS is found in the nucleus, associated with TERF1.

Tissue Specificity : Ubiquitous; highest levels in testis.

Similarity : Contains 15 ANK repeats.

Contains 1 PARP catalytic domain.

Contains 1 SAM (sterile alpha motif) domain.

抗體的鑒定：

1）端粒調(diào)控因子抗體費(fèi)用 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。

Anti-PLAUR/uPAR/FITC 熒光素標(biāo)記尿激酶型纖溶酶原激活因子受體抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rat IgM (親和純化) 大鼠IgMMulti-class antibodies規(guī)格： 1mg

血紅素氧合酶-1/熱休克蛋白-32抗體 Anti-HO-1/HSP32 0.1ml

Goat Anti-rat IgG 羊抗大鼠IgG 1mg

FoxP3 英文名稱： 叉頭蛋白P3抗體 0.1ml

Rhesus antibody Rh phospho-RelB(Ser552) 磷酸化轉(zhuǎn)錄因子RelB蛋白抗體 規(guī)格 0.1ml

Rat IgM (親和純化) 大鼠IgMMulti-class antibodies規(guī)格： 1mg

IL-13(Interleukin-13) 白介素13抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-phospho-Acinus(Ser1180) 磷酸化腺泡Acinus蛋白抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh phospho-CDKN1B/P27kip1 (Ser10) 磷酸化P27抗體/周期素依賴激酶抑制劑 規(guī)格 0.1ml

IgG 干粉 10mg/50mg/100mg 國(guó)產(chǎn)

Wnt16 英文名稱： 信號(hào)通路Wnt16抗體 0.2ml

Delta 4 英文名稱： Notch信號(hào)通路Delta樣配體4抗體 0.1ml

Anti-phospho-Acinus(Ser1180) 磷酸化腺泡Acinus蛋白抗體Multi-class antibodies規(guī)格： 0.1ml

Socs 3 (suppressor of cytokine signaling 3) 細(xì)胞因子信號(hào)傳導(dǎo)抑制蛋白3抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-BMP8 骨形態(tài)發(fā)生蛋白8抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Persephin PSP抗體 規(guī)格 0.1ml

COX2 環(huán)氧化物酶二 濃縮液 0.1ml 進(jìn)口分裝

D53 英文名稱： 蛋白D53抗體 0.2ml

C3Orf34 英文名稱： 3號(hào)染色體開(kāi)放閱讀框34抗體 0.2ml

Anti-BMP8 骨形態(tài)發(fā)生蛋白8抗體Multi-class antibodies規(guī)格： 0.2ml

人白介素15(IL-15)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

兔子硫氧化還原蛋白(x)免疫試劑盒 Rabbit Thioredoxin,x ELISA Kit

神經(jīng)型合成酶(nNOS)ELISA試劑盒 ，英文名： nNOS ELISA Kit

PorcineHydrocoisone,HYDELISA試劑盒豬(HYD)ELISA試劑盒規(guī)格：96T/48T

HumanAcylationStimulatingProtein,ASPELISA試劑盒人?；碳さ鞍?ASP)ELISA試劑盒規(guī)格：96T/48T

Humanai-Sa-aibodyELISAKit人抗Sa抗體(ai-Sa-Ab)ELISA試劑盒規(guī)格：96T/48T

大鼠酸脫氫酶E1(PDH E1) ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

人螺旋體(TP)抗體(IgM)免疫試劑盒 Human eponema pallidun(TP)aibody(IgM)ELISA kit

MousevonWillebrandFactor,vWFELISAKit小鼠性因子/瑞斯托霉素輔因子(vWF)ELISA試劑盒規(guī)格：96T/48T

石蠟切片組織CDK4蛋白表達(dá)NBT顯色光學(xué)顯微鏡檢測(cè)試劑盒10/20次

Humanproteindisulfide-isomeraseA3,PDIA3ELISA試劑盒人蛋白二硫化物異構(gòu)酶A3(PDIA3)ELISA試劑盒規(guī)格：96T/48T

humahyroidstimulatingimmunoglobulin,TSIELISAKit人甲狀腺刺激免疫球蛋白(TSI)ELISA試劑盒規(guī)格：96T/48T

端粒調(diào)控因子抗體費(fèi)用 大鼠胃動(dòng)素(MTL)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

人尾加壓素Ⅱ 免疫試劑盒 Human Urotensin Ⅱ ELISA kit

游離脂肪酸含量測(cè)試盒 可見(jiàn)分光光度法 50管/48樣

RatprocollagenⅠN-terminalpeptide,PⅠNPELISA試劑盒大鼠Ⅰ型前膠原N端前肽(PⅠNP)ELISA試劑盒規(guī)格：96T/48T

HumaniactParathormone,i-PTHELISA試劑盒人全段甲狀旁腺素(i-PTH)ELISA試劑盒規(guī)格：96T/48T

Humanmicroansferrinuria,MTFELISAKit人微量轉(zhuǎn)鐵蛋白(MTF)ELISA試劑盒規(guī)格：96T/48T