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微管蛋白抗體(免疫組化用抗體)Tubulin β費(fèi)用
  • 品牌:上海莼試
  • 產(chǎn)地:進(jìn)口、國(guó)產(chǎn)
  • 貨號(hào):CS11725
  • 發(fā)布日期: 2019-01-22
  • 更新日期: 2025-03-17
產(chǎn)品詳請(qǐng)
產(chǎn)地 進(jìn)口、國(guó)產(chǎn)
品牌 上海莼試
保存條件 Store at -20 °C
貨號(hào) CS11725
應(yīng)用范圍 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
CAS編號(hào)
抗體名 Anti-Tubulin-Beta
克隆性
靶點(diǎn) 詳見說(shuō)明書
適應(yīng)物種 詳見說(shuō)明書
形態(tài) 詳見說(shuō)明書
宿主 詳見說(shuō)明書
亞型 IgG
標(biāo)識(shí)物 詳見說(shuō)明書
濃度 1mg/1ml%
免疫原 KLH conjugated synthetic peptide derived from human Tubulin-Beta N-terminus

微管蛋白抗體(免疫組化用抗體)Tubulin β費(fèi)用 英文名稱  Anti-Tubulin-Beta

中文名稱  微管蛋白抗體(免疫組化用抗體)Tubulin β費(fèi)用 

     Beta 4 tubulin; Beta 5 tubulin; BetaTubulin; Beta Tubulin; Beta-Tubulin; dJ40E16.7; TUBB; TUBB2; TUBB2A; TUBB5; Tubulin beta chain; Tubulin beta-5 chain; TBB5_HUMAN; tubulin beta 2A.

產(chǎn)品屬性:

      1mg/1ml

規(guī)   0.1ml/100μg 0.2ml/200μg

抗體來(lái)源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 細(xì)胞骨架

蛋白分子量  predicted molecular weight: 50kDa 

       Lyophilized or Liquid

  KLH conjugated synthetic peptide derived from human Tubulin-Beta N-terminus

      IgG

純化方法   affinity purified by Protein A

儲(chǔ)    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

微管蛋白抗體(免疫組化用抗體)Tubulin β費(fèi)用 產(chǎn)品應(yīng)用   ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

(石蠟切片需做抗原修復(fù)) 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) and therefore beta Tubulin should not be used as loading control for these tissues.

Function : Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Subunit : Dimer of alpha and beta chains. May interact with RNABP10 (By similarity). Interacts with PIFO. Interacts with MX1 (By similarity).

Subcellular Location : Cytoplasm, cytoskeleton.

Tissue Specificity : Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Post-translational modifications : Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable).

Similarity : Belongs to the tubulin family.

Database links : UniProtKB/Swiss-Prot: P07437.2

結(jié)構(gòu)蛋白(Structural Proteins)

tubulin是一種大量存在于哺乳動(dòng)物腦組織中的微管亞基蛋白,在結(jié)構(gòu)上是由兩個(gè)極為相近的α和β亞基組成的二聚體、多聚體形成微管細(xì)絲,是微管的主要成分。

微管蛋白是球形分子, 有兩種類型:α微管蛋白(α-tubulin)貨號(hào):bs-0195R和β微管蛋白(β-tubulin), 這兩種微管蛋白具有相似的三維結(jié)構(gòu), 能夠緊密地結(jié)合成二聚體, 作為微管組裝的亞基。 α亞基由450個(gè)氨基酸組成, β亞基是由455個(gè)氨基酸組成, 這兩種亞基有35~40%的氨基酸序列同源, 表明編碼它們的基因可能是由同一原始祖先演變而來(lái).

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