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產(chǎn)地 | 進(jìn)口、國產(chǎn) |
品牌 | 上海莼試 |
保存條件 | Store at -20 °C |
貨號(hào) | CS11724 |
應(yīng)用范圍 | ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
CAS編號(hào) | |
抗體名 | Anti-Tubulin-beta |
克隆性 | |
靶點(diǎn) | 詳見說明書 |
適應(yīng)物種 | 詳見說明書 |
形態(tài) | 詳見說明書 |
宿主 | 詳見說明書 |
亞型 | IgG |
標(biāo)識(shí)物 | 詳見說明書 |
濃度 | 1mg/1ml% |
免疫原 | KLH conjugated synthetic peptide derived from human Tubulin-beta C-terminus |
產(chǎn)品訂購信息:
英文名稱 Anti-Tubulin-beta
中文名稱 微管蛋白抗體(免疫組化用抗體)Tubulinβ說明書
別 名 Beta 4 tubulin; Beta 5 tubulin; BetaTubulin; dJ40E16.7; TUBB; TUBB2; TUBB2A; TUBB5; tubulin beta 2A; Tubulin beta chain; TBB5_HUMAN; Tubulin beta-5 chain.
濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat
產(chǎn)品類型 一抗
研究領(lǐng)域 細(xì)胞生物 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞骨架
蛋白分子量 predicted molecular weight: 50kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Tubulin-beta C-terminus
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
微管蛋白抗體(免疫組化用抗體)Tubulinβ說明書 產(chǎn)品應(yīng)用 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) and therefore beta Tubulin should not be used as loading control for these tissues.
Function : Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Subunit : Dimer of alpha and beta chains. May interact with RNABP10 (By similarity). Interacts with PIFO. Interacts with MX1 (By similarity).
Subcellular Location : Cytoplasm > cytoskeleton.
Tissue Specificity : Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
Post-translational modifications : Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Similarity : Belongs to the tubulin family.
Database links : UniProtKB/Swiss-Prot: P07437.2
結(jié)構(gòu)蛋白(Structural Proteins)
tubulin是一種大量存在于哺乳動(dòng)物腦組織中的微管亞基蛋白,在結(jié)構(gòu)上是由兩個(gè)極為相近的α和β亞基組成的二聚體、多聚體形成微管細(xì)絲,是微管的主要成分。
微管蛋白是球形分子, 有兩種類型:α微管蛋白(α-tubulin)貨號(hào):bs-0195R和β微管蛋白(β-tubulin), 這兩種微管蛋白具有相似的三維結(jié)構(gòu), 能夠緊密地結(jié)合成二聚體, 作為微管組裝的亞基。
α亞基由450個(gè)氨基酸組成, β亞基是由455個(gè)氨基酸組成, 這兩種亞基有35~40%的氨基酸序列同源, 表明編碼它們的基因可能是由同一原始祖先演變而來。
Anti-P27/kip1 /FITC 熒光素標(biāo)記P27抗體/周期素依賴激酶抑制劑IgGMulti-class antibodies規(guī)格: 0.2ml
rec Protein A/HRP 辣根過氧化物酶標(biāo)記的重組蛋白AMulti-class antibodies規(guī)格: 0.1ml
酪蛋白激酶2相互作用蛋白1抗體 CKIP-1 0.1ml
Rabbit Anti-rat IgG/Cy5 Cy5標(biāo)記的兔抗大鼠IgG 0.1ml
Phospho-FoxO1 (Ser319) 英文名稱: 磷酸化叉頭蛋白家族1抗體 0.1ml
Rhesus antibody Rh Pin1 肽基脯氨酞順反異構(gòu)酶Pinl抗體 規(guī)格 0.1ml
rec Protein A/HRP 辣根過氧化物酶標(biāo)記的重組蛋白AMulti-class antibodies規(guī)格: 0.1ml
phospho-heterochromatin protein 1 (pTyr41)peptide 異染色質(zhì)蛋白1(磷酸化多肽)Multi-class antibodies規(guī)格: 0.5mg
Anti-ABCD1/CCL22 嗜酸粒細(xì)胞趨化蛋白22抗體Multi-class antibodies規(guī)格: 0.2ml
Rhesus antibody Rh phospho-c-Jun(Ser243) 磷酸化原癌基因c-Jun抗體 規(guī)格 0.1ml
兔抗猴IgG血清 0.5ml 國產(chǎn)
WNT5A 英文名稱: 信號(hào)通路Wnt5a抗體 0.1ml
Phospho-DBC1 (Tyr454) 英文名稱: 磷酸化缺失基因1抗體 0.1ml
Anti-ABCD1/CCL22 嗜酸粒細(xì)胞趨化蛋白22抗體Multi-class antibodies規(guī)格: 0.2ml
Anti-EV71(CT)/FITC 熒光素標(biāo)記腸道病毒71型/病毒抗體(C端)IgGMulti-class antibodies規(guī)格: 0.2ml
Anti-Nanog/FITC 熒光素標(biāo)記胚胎干細(xì)胞關(guān)鍵蛋白抗體IgGMulti-class antibodies規(guī)格: 0.2ml
Rhesus antibody Rh C17orf66 17號(hào)染色體開放閱讀框66抗體 規(guī)格 0.2ml
PSD-95 突觸后密度蛋白95(抗原) 0.5mg
Complement C3 英文名稱: 補(bǔ)體C3抗體 0.1ml
Rhesus antibody Rh ROCK2 Rho相關(guān)蛋白激酶2抗體 規(guī)格 0.1ml
Anti-Nanog/FITC 熒光素標(biāo)記胚胎干細(xì)胞關(guān)鍵蛋白抗體IgGMulti-class antibodies規(guī)格: 0.2ml
人白介素1α(IL-1α )ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝
兔子內(nèi)皮素1(ET-1)免疫試劑盒 Rabbit Endothelin 1,ET-1 ELISA Kit
蛋白(nephrin)ELISA試劑盒 ,英文名: nephrin ELISA Kit
PorcineHeatShockProtein70,HSP-70ELISA試劑盒豬熱休克蛋白70(HSP-70)ELISA試劑盒規(guī)格:96T/48T
HumanActivatedLeukocyteCellAdhesionMolecule,ALCAMELISA試劑盒人白細(xì)胞活化黏附因子(ALCAM)ELISA試劑盒規(guī)格:96T/48T
Humanai-Reticulinaibody,ARAELISAKit人抗網(wǎng)硬蛋白抗體(ARA)ELISA試劑盒規(guī)格:96T/48T
大鼠酸性成纖維細(xì)胞生長因子(aFGF/FGF-1)ELISA試劑盒 ,英文名: aFGF/FGF-1 ELISA Kit
人αL巖藻糖苷酶(AFU)ELISA檢測試劑盒Humanalpha-L-fucosidase,AFUELISAKit 96T/48T
類人猿免疫球蛋白G4(IgG4)免疫試劑盒 Simian Immunoglobulin G4,IgG4 ELISA Kit
CLIAKitforPPP1R1A(Humanproteinphosphatase1regulatorysubunitA)ELISAKit人蛋酸酶1調(diào)控/抑制因子亞基1A規(guī)格:48T/96T
石蠟切片組織EGFR蛋白表達(dá)熒光顯微鏡檢測試劑盒10/20次
ELISAKitCPSA人復(fù)合特異性抗原規(guī)格:48T/96T
微管蛋白抗體(免疫組化用抗體)Tubulinβ說明書 大鼠胃泌素(GT)ELISA試劑盒 ,英文名: GT ELISA Kit
Mouse aquaporin 2 (AQP-2) ELISA Kit 小鼠水通道蛋白2(AQP-2)ELISA試劑盒
MouselymphocytefactorELISAKit 小鼠淋巴細(xì)胞因子ELISA試劑盒 96T/48T 進(jìn)口分裝
CLIAKitforHSF1ELISAKit大鼠熱休克因子1規(guī)格:48T/96T
細(xì)胞NADPH氧化酶活性化學(xué)發(fā)光法定量檢測試劑盒20次(10樣本)
ELISAKitGABA大鼠γ氨基規(guī)格:48T/96T
抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn):
1. 微管蛋白抗體(免疫組化用抗體)Tubulinβ說明書 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析;
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件;
3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活;
4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等;
5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性;
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合;
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用;
8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。
抗體的鑒定:
1)微管蛋白抗體(免疫組化用抗體)Tubulinβ說明書 抗體的效價(jià)鑒定:不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。
2)抗體的特異性鑒定:抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力??贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗(yàn)測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3)抗體親和力:是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol??贵w親和力的測定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。