技術(shù)外包服務(wù):

★微管蛋白α/Tubulin α抗體品牌 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測序、載體構(gòu)建等

★蛋白工程：原核、哺乳動物蛋白表達系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細胞工程：細胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細胞培養(yǎng)、細胞膜制備、穩(wěn)定細胞株構(gòu)建、細胞RNAi技術(shù)等等。

實驗流程：

微管蛋白α/Tubulin α抗體品牌 英文名稱  Anti-Tubulin-alpha 1

中文名稱  微管蛋白α/Tubulin α抗體品牌 

別    名 Alpha tubulin 1; Alpha-tubulin 1; FLJ30169; H2 alpha; TBA4A_HUMAN; Testis specific alpha tubulin; Testis-specific alpha-tubulin; TUBA 4A; TUBA1; Tuba4a; Tubulin alpha 1 (testis specific); Tubulin alpha 1; Tubulin alpha 1 chain; Tubulin alpha 4a; Tubulin alpha 4A chain; Tubulin alpha-1 chain; Tubulin alpha-4A chain; Tubulin H2 alpha; Tubulin H2-alpha; TUBA4A.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat

產(chǎn)品類型   一抗  

研究領(lǐng)域    

蛋白分子量  predicted molecular weight: 55kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human Tubulin-alpha 1 C-terminus

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

微管蛋白α/Tubulin α抗體品牌 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Tubulin is a major cytoskeleton that has five distinct forms, designated alpha, beta, gamma, delta and epsilon tubulin. The alpha and beta tubulins form a heterodimer that polymerize into the cylindrical microtubule fibers. Both alpha and beta tubulin bind GTP. Only beta tubulin hydrolyzes GTP to GDP. This hydrolysis is a process that is linked to tubulin polymerization and microtubule formation. The alpha tubulin isomer can be modified by addition of a C-terminal tyrosine residue. This modification may influence polymerization rates. The gamma tubulin isomer is localized to centrosomes which compose the heart of the microtubule organizing center from which microtubule fibers emanate.

Function : Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Subunit : Dimer of alpha and beta chains.

Subcellular Location : Cytoplasm, cytoskeleton.

Post-translational modifications : Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable).

Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.

Similarity : Belongs to the tubulin family.

Database links :

UniProtKB/Swiss-Prot: P68366.1

Entrez Gene: 10376 Human

SwissProt: P68363 Human

結(jié)構(gòu)蛋白（Structural Proteins）

tubulin是一種大量存在于哺乳動物腦組織中的微管亞基蛋白，在結(jié)構(gòu)上是由兩個極為相近的α和β亞基組成的二聚體、多聚體形成微管細絲，是微管的主要成分。

微管蛋白是球形分子, 有兩種類型:α微管蛋白(α-tubulin)和β微管蛋白(β-tubulin)貨號：bs-0210R, 這兩種微管蛋白具有相似的三維結(jié)構(gòu), 能夠緊密地結(jié)合成二聚體, 作為微管組裝的亞基。

α亞基由450個氨基酸組成, β亞基是由455個氨基酸組成, 這兩種亞基有35～40%的氨基酸序列同源, 表明編碼它們的基因可能是由同一原始祖先演變而來.

全、新、優(yōu)、品、好四大特點：

微管蛋白α/Tubulin α抗體品牌 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標準品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

好：我公司具有優(yōu)質(zhì)的技術(shù)團隊，產(chǎn)品一旦售出，實驗過程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時沒有任何的后顧之憂。

正在熱銷的相關(guān)產(chǎn)品：

Anti-PEA3/FITC 熒光素標記多瘤促活化因子抗體IgGMulti-class antibodies規(guī)格： 0.2ml

rec Protein G/FITC 熒光素標記重組純化蛋白GMulti-class antibodies規(guī)格： 0.3ml

上皮細胞粘附分子/表皮細胞粘附分子抗體 Anti-EpCAM 0.2ml

Donkey Anti-Guinea pig IgG/Alexa Fluor 555 Alexa Fluor 555標記的驢抗豚鼠IgG 0.1ml

factor VIII 英文名稱： 凝血因子8/第八凝血因子/第八因子相關(guān)抗原抗體 0.1ml

Rhesus antibody Rh phospho-Tau protein(Thr205) 磷酸化微管相關(guān)蛋白抗體 規(guī)格 0.1ml

rec Protein G/FITC 熒光素標記重組純化蛋白GMulti-class antibodies規(guī)格： 0.3ml

IL-2 (Interleukin-2)Mouse、Ret 白介素2抗原(大、小鼠)Multi-class antibodies規(guī)格： 0.5mg

Anti-AD7c-NTP 神經(jīng)絲蛋白抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Phospho-CDK2 (Thr160) 磷酸化周期素依賴性激酶2抗體 規(guī)格 0.1ml

免疫組化筆 支 進口

Wnt6 英文名稱： 信號通路Wnt6抗體 0.2ml

DAAM2 英文名稱： 形態(tài)發(fā)生紊亂關(guān)聯(lián)激活因子2抗體 0.1ml

Anti-AD7c-NTP 神經(jīng)絲蛋白抗體Multi-class antibodies規(guī)格： 0.2ml

Anti-Phospho-IKK alpha/beta (Ser176/Ser180) /FITC 熒光素標記磷酸化核因子κB激酶alpha/beta抑制劑抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-pro-caspase3/FITC 熒光素標記半胱天冬酶-3酶原抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Apollon/BIRC6 Apollon凋亡抑制蛋白抗體 規(guī)格 0.2ml

Rabbit Anti-pig IgG/AP 堿性磷酸酶(AP)標記的兔抗豬IgG 0.1ml

Cobra poison Protein 英文名稱： 眼鏡蛇蛇毒蛋白抗體 0.2ml

Rhesus antibody Rh Rabbit anti-MG IgG/Gold 膠體金標記的兔抗長爪沙鼠IgG 規(guī)格 0.5ml

Anti-pro-caspase3/FITC 熒光素標記半胱天冬酶-3酶原抗體IgGMulti-class antibodies規(guī)格： 0.2ml

人白介素1β (IL-1β)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

兔子內(nèi)皮抑素(ES)免疫試劑盒 Rabbit Endostatin,ES ELISA Kit

腎上腺皮質(zhì)抗體(ACA)ELISA試劑盒 ，英文名： ACA ELISA Kit

PorcineHeatShockProtein40,HSP-40ELISA試劑盒豬熱休克蛋白40(HSP-40)ELISA試劑盒規(guī)格：96T/48T

HumanactivatedcoagulationfactorⅫ,FⅫaELISA試劑盒人活化凝血因子Ⅻ(FⅫa)ELISA試劑盒規(guī)格：96T/48T

Humanai-respiratorysyncytialvirusaibody，RSVELISAKit人抗合胞病毒抗體(RSV)ELISA試劑盒規(guī)格：96T/48T

大鼠酸性成纖維細胞生長因子(FGF1)ELISA試劑盒 ，英文名： FGF1 ELISA Kit

Mouse ferritin (FE) ELISA Kit 小鼠鐵蛋白(FE)ELISA試劑盒

ratEndothelin1,ET-1ELISAKit 大鼠內(nèi)皮素1(ET-1)ELISA試劑盒 96T/48T 進口分裝

CLIAKitforhiston-H2bELISAKit大鼠組蛋白H2b規(guī)格：48T/96T

細胞ROCK-I激酶活性定量檢測試劑盒(A/B/C)20次

ELISAKitPⅢNP大鼠Ⅲ型前膠原肽規(guī)格：48T/96T

微管蛋白α/Tubulin α抗體品牌 大鼠胃泌素(GT/GAS)ELISA試劑盒 96T/48T 試劑盒 組裝/原裝

人尾肢同源蛋白2(PYGO2/PP7910)免疫試劑盒 Human pygopus homolog 2,PYGO2/PP7910 ELISA kit

糜蛋白酶測試盒 可見分光光度法 50管/48樣

RatProAialNaiureticPeptide,Pro-ANPELISA試劑盒大鼠前心肽(Pro-ANP)ELISA試劑盒規(guī)格：96T/48T

Humaninsulin-likegrowthfactorsbindingprotein4,IGFBP-4ELISA試劑盒人樣生長因子結(jié)合蛋白4(IGFBP-4)ELISA試劑盒規(guī)格：96T/48T

Humanmicroalbunminuria,MAU/ALBELISAKit人尿微量白蛋白(ALB)ELISA試劑盒規(guī)格：96T/48T