產(chǎn)品訂購(gòu)信息：
英文名稱  Anti-SynCAM/TSLC1 

中文名稱   細(xì)胞粘附分子1抗體說(shuō)明書(shū) 

別    名  CADM1; BL2; Cadm1; CADM1_HUMAN; Cell adhesion molecule 1; DKFZp686F1789; IGSF4; IGSF4A; Immunoglobulin Superfamily Member 4; MGC149785; MGC51880; NECL-2; NECL2; Nectin like 2; Nectin-like protein 2; RA175; SgIGSF; Spermatogenic immunoglobulin superfamily; ST17; sTSLC 1; Synaptic cell Adhesion Molecule; SynCAM; SynCAM1; TSLC-1; TSLC1; Tumor Suppressor in Lung Cancer 1.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來(lái)源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog 

產(chǎn)品類型   一抗  

研究領(lǐng)域     免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo)

蛋白分子量  predicted molecular weight: 44kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human TSLC1 C-terminus 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

細(xì)胞粘附分子1抗體說(shuō)明書(shū) 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca(2+)-independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. May act as a synaptic cell adhesion molecule that drives synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.

Function : Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca(2+)-independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. May act as a synaptic cell adhesion molecule that drives synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.

Subunit : Homodimer. Interacts with CRTAM and EPB41L3/DAL1. The interaction with EPB41L3/DAL1 may act to anchor CADM1 to the actin cytoskeleton. Interacts via its C-terminus with the PDZ domain of MPP3 and the PDZ domain of MPP6.

Subcellular Location : Cell membrane; Single-pass type I membrane protein. Note=Associates with perinuclear and plasma membranes in vivo. Localized to the basolateral plasma membrane of epithelial cells in gall bladder.

Similarity : Belongs to the nectin family.

Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Database links : UniProtKB/Swiss-Prot: Q9BY67.2

Entrez Gene: 23705 Human

Entrez Gene: 54725 Mouse

Entrez Gene: 363058 Rat

Omim: 605686 Human

SwissProt: Q9BY67 Human

SwissProt: Q8R5M8 Mouse

Unigene: 370510 Human

Unigene: 234832 Mouse

TSLC1屬于新的基因免疫球蛋白超家族, TSLC1蛋白質(zhì)參與細(xì)胞間黏附、細(xì)胞運(yùn)動(dòng)、信號(hào)轉(zhuǎn)導(dǎo)及. TSLC1在多種中表達(dá)異常,它對(duì)的影響主要表現(xiàn)為特性及基因表達(dá),可抑制上皮間質(zhì)轉(zhuǎn)化.

TSLC1蛋白在控制生物侵襲性方面有著重要作用，也可能作為預(yù)測(cè)患者預(yù)后的重要生物標(biāo)記，但是TSLC1的抑制分子機(jī)制還有待進(jìn)一步研究。

Anti-JNK1/2/3/FITC 熒光素標(biāo)記抗氨基末端激酶1/2/3抗體 IgGMulti-class antibodies規(guī)格： 0.2ml

Recomb Protein A/Gold 金標(biāo)記重組蛋白A(10nm/15nm)Multi-class antibodies規(guī)格： 0.5ml

叉頭蛋白3-T細(xì)胞轉(zhuǎn)錄因子 Anti-FoxP3 0.1ml

Goat Anti-rabbit IgG/PE PE標(biāo)記的羊抗兔IgG 0.1ml

FMO5 英文名稱： 二甲基苯胺單加氧酶5抗體 0.2ml

Rhesus antibody Rh phospho-STAT3 (Ser727) 磷酸化信號(hào)轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子3抗體 規(guī)格 0.1ml

Recomb Protein A/Gold 金標(biāo)記重組蛋白A(10nm/15nm)Multi-class antibodies規(guī)格： 0.5ml

IL-4(Interleukin-4) 白介素4抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-ADAMTS1 整合素樣金屬蛋白酶與凝血酶1型抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh phospho-Cdc6 (Ser54) 磷酸化細(xì)胞分裂周期蛋白6抗體 規(guī)格 0.1ml

免疫組化/原位雜交專用波片(雙面) 50片 真空車間清洗硅化處理,表面無(wú)污漬

Wnt8A 英文名稱： 信號(hào)通路WNT8A抗體 0.2ml

DUOXA2 英文名稱： 雙氧化酶激活因子2抗體 0.2ml

Anti-ADAMTS1 整合素樣金屬蛋白酶與凝血酶1型抗體Multi-class antibodies規(guī)格： 0.1ml

OJ/IleRS(Human anti-OJ-antibody) ELISA Kit 人OJ抗體/抗異亮氨酰tRNA合成酶抗體Multi-class antibodies規(guī)格： 48T

Anti-Phospho-PLC gamma 2 (Tyr1217) 磷酸化磷酯酶Cγ2抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh HHV8/ORF50 人類病毒8抗體 規(guī)格 0.2ml

ENA-78/CXCL5 ELISA Kit 大鼠上皮中性粒細(xì)胞活化肽78 96T

Classical swine fever virus 英文名稱： 抗體 0.2ml

C20orf26 英文名稱： 20號(hào)染色體開(kāi)放閱讀框26抗體 0.2ml

Anti-Phospho-PLC gamma 2 (Tyr1217) 磷酸化磷酯酶Cγ2抗體Multi-class antibodies規(guī)格： 0.1ml

人白介素1可溶性受體Ⅱ(IL-1sRⅡ)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

兔子凝血因子Ⅱ(FⅡ)免疫試劑盒 Rabbit coagulation factor Ⅱ,FⅡ ELISA Kit

腎上腺髓質(zhì)素(ADM)ELISA試劑盒 ，英文名： ADM ELISA Kit

Porcinegrowthhormonereleasingpeptide-Ghrelin,GHRP-GhrelinELISA試劑盒豬生長(zhǎng)激素釋放肽ghrelin(GHRP-Ghrelin)ELISA試劑盒規(guī)格：96T/48T

Humanacidicfibroblastgrowthfactor1,aFGF-1ELISA試劑盒人酸性成纖維細(xì)胞生長(zhǎng)因子1(aFGF-1)ELISA試劑盒規(guī)格：96T/48T

Humanai-Qfeveraibody,ai-Q-AbELISAKit人抗Q熱抗體(ai-Q-Ab)ELISA試劑盒規(guī)格：96T/48T

大鼠酸性磷酸酶(ACP)ELISA試劑盒 ，英文名： ACP ELISA Kit

人熱休克蛋白70(HSP-70)ELISA檢測(cè)試劑盒HumanHeatShockProtein70,HSP-70ELISAKit 96T/48T

鹿γ干擾素(IFNG)免疫試劑盒 Deer ierferon gamma,IFNG ELISA kit

CLIAKitforPR3Ab(HumanProteinase3Aibody)ELISAKit人蛋白酶3抗體規(guī)格：48T/96T

石蠟切片組織DAPKINASE蛋白表達(dá)熒光顯微鏡檢測(cè)試劑盒10/20次

ELISAKitPNP人副性天皰瘡抗體規(guī)格：48T/96T

細(xì)胞粘附分子1抗體說(shuō)明書(shū) 大鼠吻素受體(KISS1R)ELISA試劑盒 ，英文名： KISS1R ELISA Kit

Mouse dihydrotestosterone (DHT) ELISA Kit 小鼠雙氫睪酮(DHT)ELISA試劑盒

MouseSmad7ELISAKit 小鼠信號(hào)轉(zhuǎn)導(dǎo)分子7(Smad7)ELISA試劑盒 96T/48T 進(jìn)口分裝

CLIAKitforHSL(Humanhormonesensitivelipase)ELISAKit人激素敏感性脂肪酶規(guī)格：48T/96T

細(xì)胞NADPH細(xì)胞色素C還原酶(NADPHCytochromeCReductase)活性比色法定量檢測(cè)試劑盒20次

ELISAKitOrexinA大鼠阿立新A規(guī)格：48T/96T

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 細(xì)胞粘附分子1抗體說(shuō)明書(shū) 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；

3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）細(xì)胞粘附分子1抗體說(shuō)明書(shū) 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。