產(chǎn)品訂購信息：
英文名稱  Anti-TAS2R7/T2R6 

中文名稱   味覺感受器蛋白T2R6抗體費用 

別    名  MGC142121; mT2R42; STC7 4; T2R30; T2R6; T2R7; TA2R7_HUMAN; Tas2r130; Tas2r6; TAS2R7; Taste receptor family B member 4; Taste receptor type 2 member 7; TRB4.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 神經(jīng)生物學(xué) 通道蛋白 G蛋白偶聯(lián)受體 G蛋白信號

蛋白分子量  predicted molecular weight: 36kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from hu TAS2R7/T2R6 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

味覺感受器蛋白T2R6抗體費用 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The sense of taste provides animals with valuable information about the quality and nutritional value of food. A family of G protein-coupled receptors are involved in taste perception and include T1R, which is involved in sweet and umami taste perception, and T2R, which is involved in bitter taste perception. Both types of taste receptors couple to various G proteins to initiate signal transduction cascades. Single taste receptor cells express a variety of T2Rs, suggesting that each cell is capable of recognizing multiple tastants. T2R6 (also designatedT2R30, mt2r42, STC 7-4 or taste receptor, type 2, member 130) is an integral membrane receptor protein in mice that may play a role in the perception of bitterness and in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate ?-gustducin, mediate PLC-∫-2 activation and lead to the gating of TRPM5. T2R6 is expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells. The human homolog of T2R6, designated T2R7 (TAS2R7, TRB4 or taste receptor, type 2, member 7) is a G protein-coupled receptor expressed in taste receptor cells of the tongue and palate epithelia.

Function : Gustducin-coupled receptor implicated in the perception of bitter compounds in the oral cavity and the gastrointestinal tract. Signals through PLCB2 and the calcium-regulated cation channel TRPM5.

Subunit : Belongs to the G-protein coupled receptor T2R family.

Subcellular Location : Membrane.

Tissue Specificity : Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells.

Similarity : Belongs to the G-protein coupled receptor T2R family.

Anti-SARS S-protein/FITC 熒光素標(biāo)記病毒表面蛋白S抗體IgGMulti-class antibodies規(guī)格： 0.2ml

ShK(Stichodactyla helianthus Neurotoxin)-(Gln9) ?？窠?jīng)毒素(35肽)Multi-class antibodies規(guī)格： 0.1mg

基質(zhì)金屬蛋白酶-7抗體 Anti-MMP-7 0.1ml

alpha-Synuclein (NT) 英文名稱： 核突觸蛋白-α抗體(N端) 0.1ml

FGF10 英文名稱： 成纖維細(xì)胞生長因子10抗體 0.1ml

Rhesus antibody Rh Phospho-PDGFRA(Tyr849)/PDGFRB(Tyr857) 磷酸化血小板源性生長因子受體α/β抗體 規(guī)格 0.1ml

ShK(Stichodactyla helianthus Neurotoxin)-(Gln9) ?？窠?jīng)毒素(35肽)Multi-class antibodies規(guī)格： 0.1mg

phospho-GSK3 Alpha/ Beta(alpha + beta)(phospho Tyr279 + Tyr216) 磷酸化葡萄糖合成激酶3α/β抗原Multi-class antibodies規(guī)格： 0.5mg

SCARB2 LIMP-2 / SCARB2 / CD36L2 兔單抗 (PE) Mouse

Rhesus antibody Rh phospho-CREB-1(Ser142) 磷酸化CREB-1抗體 規(guī)格 0.1ml

總RNA抽提試劑 100ml 國產(chǎn)

ZA20D3 英文名稱： 鋅指蛋白20D3抗體 0.2ml

DLX2 英文名稱： 同源轉(zhuǎn)錄因子DLX2抗體 0.2ml

SCARB2 LIMP-2 / SCARB2 / CD36L2 兔單抗 (PE) Mouse

INS ELISA Kit 大鼠Multi-class antibodies規(guī)格： 48T

Anti-Na,K-ATPase alpha-1 (Phospho Ser23) /FITC 熒光素標(biāo)記兔抗人、大、小鼠磷酸化ATP酶蛋白a1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh FABPB 腦型脂肪酸結(jié)合蛋白抗體 規(guī)格 0.2ml

pACCase ELISA Kit 大鼠磷酸化乙酰輔酶A羧化酶 96T

CCL3 英文名稱： 巨噬細(xì)胞因子1α抗體 MIP-1α 0.1ml

AP2 beta 英文名稱： 銜接蛋白β抗體 0.1ml

Anti-Na,K-ATPase alpha-1 (Phospho Ser23) /FITC 熒光素標(biāo)記兔抗人、大、小鼠磷酸化ATP酶蛋白a1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

人白三烯E4(LTE4)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

兔子髓磷脂堿性蛋白(MBP)免疫試劑盒 Rabbit myelin basic protein,MBP ELISA Kit

視黃醇結(jié)合蛋白4(RBP4)ELISA試劑盒 ，英文名： RBP4 ELISA Kit

Porcinecholecystokinin,CCKELISA試劑盒豬膽囊收縮素/腸促胰酶肽(CCK)ELISA試劑盒規(guī)格：96T/48T

Human2,3-dinohromboxaneB2,2,3-dinor-TXB2ELISA試劑盒人2,3Dinor烷B2(2,3-dinor-TXB2)ELISA試劑盒規(guī)格：96T/48T

Humanai-macrophageaibodyELISAKit人抗巨噬細(xì)胞抗體(ai-macrophageAb)ELISA試劑盒規(guī)格：96T/48T

大鼠胎兒腎鈣黏蛋白(KCAD)ELISA試劑盒 ，英文名： KCAD ELISA Kit

Bovine ceruloplasmin (CP) ELISA Kit 牛銅藍(lán)蛋白(CP)ELISA試劑盒

Ratverylateappearingaigen,VLAELISAKit 大鼠遲現(xiàn)抗原(VLA)ELISA試劑盒 96T/48T 進(jìn)口分裝

CLIAKitforHL(Mousehepaticlipase)ELISAKit小鼠肝脂酶規(guī)格：48T/96T

細(xì)胞PKG-I激酶活性定量檢測試劑盒(A/B/C)20次

ELISAKitBcl-2大鼠B細(xì)胞因子2規(guī)格：48T/96T

味覺感受器蛋白T2R6抗體費用 大鼠細(xì)胞間粘附分子5(ICAM5)ELISA試劑盒 ，英文名： ICAM5 ELISA Kit

Mouse growth hormone releasing peptide ghrelin (GHRP-Ghrelin) ELISA Kit 小鼠生長激素釋放肽ghrelin(GHRP-Ghrelin)ELISA試劑盒

MouseTissuefactorpathwayinhibitor,TFPIELISAKit 小鼠組織因子途徑抑制物(TFPI)ELISA試劑盒 96T/48T 進(jìn)口分裝

CLIAKitforHsp-40(MouseHeatShockProtein40)ELISAKit小鼠熱休克蛋白40規(guī)格：48T/96T

細(xì)胞MAPKAPKINASE2激酶活性定量檢測試劑盒(A/B/C)20次

ELISAKitIL-18大鼠白介素18規(guī)格：48T/96T

抗體的生物素化標(biāo)記實驗要點：

1. 味覺感受器蛋白T2R6抗體費用 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）味覺感受器蛋白T2R6抗體費用 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴(kuò)散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。