產(chǎn)品訂購信息：
英文名稱  Anti-BTRC2/beta TRCP2 

中文名稱   BTRC2蛋白抗體品牌 

別    名  beta Transducin Repeat Containing Protein 2; BTRC2; BTRCP2; F-box and WD-40 Domain Protein 1B; F-box Protein FBW1B; FBW1B; FBXW1B; FBW1B_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應   Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Zebrafish 

產(chǎn)品類型   一抗  

研究領域    細胞生物 免疫學 細胞周期蛋白

蛋白分子量  predicted molecular weight: 62kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human BTRC2/beta TRCP2 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

BTRC2蛋白抗體品牌 產(chǎn)品應用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Beta TRCP2 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class and, in addition to an F-box, contains multiple WD40 repeats. This gene contains at least 14 exons, and its alternative splicing generates 3 transcript variants diverging at the presence/absence of two alternate exons.

Function : Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22'. SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1/nuclear factor NF-kappa-B p105 subunit, ATF4, SMAD3, SMAD4, CDC25A, DLG1, FBXO5 and probably NFKB2. SCF(BTRC) mediates the ubiquitination of phosphorylated SNAI1. May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase. Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF. Required for proteolytic processing of GLI3.

Subunit : Self-associates. Component of the SCF(FBXW11) complex formed of CUL1, SKP1, RBX1 and a FBXW11 dimer. Interacts with BTRC, BST2, PER1, RCAN1 and USP47. Interacts with phosphorylated ubiquitination substrate PER2 (By similarity). Interacts with phosphorylated ubiquitination substrates CTNNB1, NFKBIA, IFNAR1; the interaction requires the phosphorylation of the two serine residues in the substrates' destruction motif D-S-G-X(2,3,4)-S. Interacts with TRIM21.

Subcellular Location : Cytoplasm. Nucleus.

Similarity : Contains 1 F-box domain.

Contains 7 WD repeats.

Database links : UniProtKB/Swiss-Prot: Q9UKB1.1

Anti-TSLC1/FITC 熒光素標記阻抑基因1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

TrK A.B.C 神經(jīng)生長因子的一種(抗原)Multi-class antibodies規(guī)格： 0.5mg

X-連鎖凋亡蛋白/性連鎖凋亡抑制蛋白抗體 anti-XIAP 0.2ml

SCG10 英文名稱： 神經(jīng)生長相關蛋白SCG10抗體 0.1ml

EDA 英文名稱： 外胚層蛋白1抗體 0.2ml

Rhesus antibody Rh phospho-IKK beta(Ser466) 磷酸化KB抑制蛋白激酶β抗體 規(guī)格 0.1ml

TrK A.B.C 神經(jīng)生長因子的一種(抗原)Multi-class antibodies規(guī)格： 0.5mg

FN(Mouse Fibronectin) ELISA Kit 小鼠纖連蛋白Multi-class antibodies規(guī)格： 48T

Anti-CCL23/MPIF-1/FITC 熒光素標記因子1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh EDNRA 內(nèi)皮素受體A抗體 規(guī)格 0.1ml

Fish Cortisol ELISA Kit 魚類皮質醇 96T

ZNF 英文名稱： 鋅指蛋白抗體 0.2ml

phospho-ATF2 (Thr51 + Thr53) 英文名稱： 磷酸化活化復制因子2抗體 0.1ml

Anti-CCL23/MPIF-1/FITC 熒光素標記因子1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

MAPK1/ERK3 (Mitogen-activated protein kinase 3; P44MAPK) 原活化蛋白激酶3抗原Multi-class antibodies規(guī)格： 0.5mg

緩激肽B2受體抗體 Anti-Bdkrb2/B2R 0.1ml

Rhesus antibody Rh phospho-EGFR (Tyr1092) 磷酸化表皮生長因子受體抗體 規(guī)格 0.1ml

N2添加劑 (原裝) 5ml Gibco

ANKZF1 英文名稱： 鋅指蛋白744抗體 0.2ml

DVL1 英文名稱： 蓬亂蛋白1抗體 0.1ml

緩激肽B2受體抗體 Anti-Bdkrb2/B2R 0.1ml

人鞭毛蛋白(flagellin)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

兔子組織多肽抗原(TPA)免疫試劑盒 Rabbit Tissue Polypeptide Aigen,TPA ELISA Kit

絲酸蛋白酶HA1(HA1)ELISA試劑盒 ，英文名： HA1 ELISA Kit

Nasoniaviipennisvitellogenin,VtgELISA試劑盒蝶蛹金小蜂卵黃蛋白原(vtg)ELISA試劑盒規(guī)格：96T/48T

guineapighepatocytegrowthfactor,HGFELISA試劑盒豚鼠肝細胞生長因子(HGF)ELISA試劑盒規(guī)格：96T/48T

HumanAi-Compleme1qaibody,C1qELISAKit人抗補體1q抗體(C1q)ELISA試劑盒規(guī)格：96T/48T

大鼠糖原磷酸化酶M(PYGM)ELISA試劑盒 ，英文名： PYGM ELISA Kit

Mouse placea growth factor (PLGF) ELISA Kit 小鼠胎盤生長因子(PLGF)ELISA試劑盒

Rat17-Hydroxyprogesterone,17-OHPELISAKit 大鼠17羥孕酮(17-OHP)ELISA試劑盒 96T/48T 進口分裝

CLIAKitforH-4(umanNeuroophin4)ELISAKIT人神經(jīng)營養(yǎng)因子4規(guī)格：48T/96T

細胞PKCδ激酶活性定量檢測試劑盒(A/B/C)20次

ELISAKi-proBNP大鼠N端前腦素規(guī)格：48T/96T

BTRC2蛋白抗體品牌 大鼠細胞周期素D3(Cyclin-D3)ELISA試劑盒 ，英文名： Cyclin-D3 ELISA Kit

人輸血傳播病毒/辛型肝炎病毒((TTV)ELISA檢測試劑盒humanansfusionansmittedvirus,TTVELISAKit 96T/48T

大鼠Ⅲ型前膠原氨基末端肽(PⅢNP)免疫試劑盒 Rat N-terminal procollagen Ⅲ propeptide,PⅢNP ELISA Kit

CLIAKitforSAA(RabbitSerumamyloidA)ELISAKit兔血清淀粉樣蛋白A規(guī)格：48T/96T

石蠟切片結締組織(CONNECTIVETISSUE)馬森(MASSON)染色試劑盒50次

ELISAKitTn-Ⅰ人肌鈣蛋白Ⅰ規(guī)格：48T/96T

抗體的生物素化標記實驗要點：

1. BTRC2蛋白抗體品牌 如在反應混合液中有疊氮鈉或游離氨基存在,會抑制標記反應。因此,蛋白質在反應前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質之間的分子比按蛋白質表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結合位點可能因此被封閉,導致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結合；

7.交聯(lián)反應后,應充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結合產(chǎn)生競爭作用；

8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導致高本底。

抗體的鑒定：

1）BTRC2蛋白抗體品牌 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應,瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應抗原或近似抗原物質的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應率來表示。交叉反應率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結合率,求出各自在IC50時的濃度,并按公式計算交叉反應率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質的交叉反應率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結合位點與抗原決定簇之間立體構型的合適度決定的。有助于維持抗原抗體復合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。