產(chǎn)品訂購信息：
英文名稱  Anti-TWF2/PTK9L 

中文名稱   細胞骨架結(jié)合蛋白2抗體說明書 

別    名  A6 related protein; A6r; A6RP; hA6RP; MSTP011; Protein tyrosine kinase 9 like; PTK 9L; PTK9L;PTK9L; PTK9L protein tyrosine kinase 9 like; TWF 2; Twinfilin 1 like protein; Twinfilin 2; Twinfilin actin binding protein homolog 2; Twinfilin like protein; Twinfilin2; TWF2_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep 

產(chǎn)品類型   一抗  

研究領(lǐng)域    神經(jīng)生物學 結(jié)合蛋白 細胞骨架

蛋白分子量  predicted molecular weight: 39kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human TWF2/PTK9L 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

細胞骨架結(jié)合蛋白2抗體說明書 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Twinfilin-2 is a 349 amino acid protein that localizes to the perinuclear region of the cytoplasm, as well as to the cytoskeleton, and contains two ADF-H domains. Expressed ubiquitously, Twinfilin-2 functions as an Actin-binding protein that is able to both bind and sequester G-Actin and cap the barbed ends of Actin filaments, thereby inhibiting Actin polymerization and playing a role in Actin-related motile and morphological processes. Twinfilin-2 is subject to post-translational phosphorylation by PKC zeta. The gene encoding Twinfilin-2 maps to human chromosome 3, which houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci.

Function : TWF2 (Twinfilin 2) was identified by its interaction with the catalytic domain of protein kinase C zeta. It contains an actin binding site and an ATP binding site and is most closely related to twinfilin (PTK9), a conserved actin monomer binding protein. TWF2 is an actin binding protein involved in motile and morphological processes. It inhibits actin polymerization, likely by sequestering G actin. By capping the barbed ends of filaments, it also regulates motility. It seems to play an important role in clathrin mediated endocytosis and distribution of endocytic organelles.

Subunit : Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF1 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down-regulated. Interacts with MYO7A

Subcellular Location : Cytoplasmic, cytoskeleton. Cytoplasmic, perinuclear region. Note: Perinuclear and G actin rich cortical actin structures sublocalization.

Tissue Specificity : Ubiquitously expressed (at protein level).

Post-translational modifications : In vitro, phosphorylated by PRKCZ, CK2 and SRC.

Similarity : Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.

Contains 2 ADF-H domains.

Database links : UniProtKB/Swiss-Prot: Q6IBS0.2

AFP ELISA Kit 大鼠甲種胎兒球蛋白/甲胎蛋白Multi-class antibodies規(guī)格： 48T

單抗 單抗Multi-class antibodies規(guī)格：

Rhesus antibody Rh FRMD1 FRMD1蛋白抗體 規(guī)格 0.2ml

AGEs ELISA Kit 大鼠晚期糖基化終末產(chǎn)物 96T

Neurofascin 英文名稱： 神經(jīng)束蛋白抗體 0.1ml

AHNAK 英文名稱： 神經(jīng)細胞分化相關(guān)蛋白AHNAK抗體 0.1ml

單抗 單抗Multi-class antibodies規(guī)格：

phospho-FAK(pTyr577) 磷酸化粘著斑激酶抗原Multi-class antibodies規(guī)格： 0.5mg

橋連整合因子蛋白抗體 Anti-BIN1 0.1ml

Rhesus antibody Rh Phospho-EEF2 (Thr56) 磷酸化真核翻譯延長因子2抗體 規(guī)格 0.1ml

品名 規(guī)格 備注

ZNF342 英文名稱： 鋅指蛋白342抗體 0.2ml

phospho-DAP Kinase 1 (Ser 308) 英文名稱： 磷酸化凋亡相關(guān)蛋白激酶1抗體 0.1ml

橋連整合因子蛋白抗體 Anti-BIN1 0.1ml

Anti-phospho-FOXO4 (phospho S197) /FITC 熒光素標記兔抗人、大、小鼠叉頭蛋白4抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-NKR/Neurokin B receptor /FITC 熒光素標記神經(jīng)激肽-B受體抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh BTBD1/HCV NS5A transactivated protein 8 丙毒NS5A反轉(zhuǎn)錄蛋白8抗體 規(guī)格 0.2ml

ASCL1/MASH1(Achaete-scute homolog 1) 神經(jīng)母細胞特異性轉(zhuǎn)移因子抗原 0.5mg

Acetyl-Histone H4(K8) 英文名稱： 乙酰化組蛋白H4抗體 0.1ml

Rhesus antibody Rh RIPK3 受體結(jié)合絲氨酸蘇氨酸激酶3抗體 規(guī)格 0.1ml

Anti-NKR/Neurokin B receptor /FITC 熒光素標記神經(jīng)激肽-B受體抗體IgGMulti-class antibodies規(guī)格： 0.2ml

人型肝炎IgM(HCV-IgM)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

豚鼠白介素5(IL-5)免疫試劑盒 Guinea pig ierleukin 5,IL-5 ELISA Kit

髓磷脂堿性蛋白(MBP)ELISA試劑盒 ，英文名： MBP ELISA Kit

MouseVitaminB6,VB6ELISA試劑盒小鼠維生素B6(VB6)ELISA試劑盒規(guī)格：96T/48T

FishOsteocalcin/Boneglaprotein,OT/BGPELISA試劑盒魚骨鈣素/骨谷氨酸蛋白(OT/BGP)ELISA試劑盒規(guī)格：96T/48T

HumanankyrinB,Ank-BELISAKit人錨蛋白B(Ank-B)ELISA試劑盒規(guī)格：96T/48T

大鼠同型半胱酸(HA)ELISA試劑盒 ，英文名： HA ELISA Kit

Bovine glucose 6 phosphate dehydrogenase (G6PD) ELISA Kit 牛葡萄糖6磷酸脫氫酶(G6PD)ELISA試劑盒

RatNeonatalThyroxine,NN-T4ELISAKit 大鼠新生甲狀腺素(NN-T4)ELISA試劑盒 96T/48T 進口分裝

CLIAKitforHO-2(Humanhemeoxygenase2)ELISAKit人血紅素氧合酶2規(guī)格：48T/96T

細胞PKA激酶活性定量檢測試劑盒(A/B/C)20/50次

ELISAKitP-Selectin/CD62P大鼠P選擇素規(guī)格：48T/96T

細胞骨架結(jié)合蛋白2抗體說明書 大鼠纖維蛋白肽B(FPB)ELISA試劑盒 ，英文名： FPB ELISA Kit

Mouse chorionic gonadoopin (CG) ELISA Kit 小鼠絨毛膜激素(CG)ELISA試劑盒

Mousemaixmetalloproteinase5,MMP-5ELISAKit 小鼠基質(zhì)金屬蛋白酶5(MMP-5)ELISA試劑盒 96T/48T 進口分裝

CLIAKitforHuman2,3-dinohromboxaneB2,2,3-dinor-TXB2ELISAKit人2,3Dinor烷B2規(guī)格：48T/96T

細胞HSV(HERPESSIMPLX)病毒定性檢測試劑盒20次

ELISAKitEGF大鼠表皮生長因子規(guī)格：48T/96T

抗體的生物素化標記實驗要點：

1. 細胞骨架結(jié)合蛋白2抗體說明書 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導致高本底。

抗體的鑒定：

1）細胞骨架結(jié)合蛋白2抗體說明書 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。