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產(chǎn)地 | 進口、國產(chǎn) |
品牌 | 上海莼試 |
保存條件 | Store at -20 °C |
貨號 | CS11636 |
應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 |
CAS編號 | |
抗體名 | Anti-TWF2/PTK9L |
克隆性 | |
靶點 | 詳見說明書 |
適應(yīng)物種 | 詳見說明書 |
形態(tài) | 詳見說明書 |
宿主 | 詳見說明書 |
亞型 | IgG |
標識物 | 詳見說明書 |
濃度 | 1mg/1ml% |
免疫原 | KLH conjugated synthetic peptide derived from human TWF2/PTK9L |
產(chǎn)品訂購信息:
英文名稱 Anti-TWF2/PTK9L
中文名稱 細胞骨架結(jié)合蛋白2抗體說明書
別 名 A6 related protein; A6r; A6RP; hA6RP; MSTP011; Protein tyrosine kinase 9 like; PTK 9L; PTK9L;PTK9L; PTK9L protein tyrosine kinase 9 like; TWF 2; Twinfilin 1 like protein; Twinfilin 2; Twinfilin actin binding protein homolog 2; Twinfilin like protein; Twinfilin2; TWF2_HUMAN.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep
產(chǎn)品類型 一抗
研究領(lǐng)域 神經(jīng)生物學 結(jié)合蛋白 細胞骨架
蛋白分子量 predicted molecular weight: 39kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human TWF2/PTK9L
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
細胞骨架結(jié)合蛋白2抗體說明書 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
(石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Twinfilin-2 is a 349 amino acid protein that localizes to the perinuclear region of the cytoplasm, as well as to the cytoskeleton, and contains two ADF-H domains. Expressed ubiquitously, Twinfilin-2 functions as an Actin-binding protein that is able to both bind and sequester G-Actin and cap the barbed ends of Actin filaments, thereby inhibiting Actin polymerization and playing a role in Actin-related motile and morphological processes. Twinfilin-2 is subject to post-translational phosphorylation by PKC zeta. The gene encoding Twinfilin-2 maps to human chromosome 3, which houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci.
Function : TWF2 (Twinfilin 2) was identified by its interaction with the catalytic domain of protein kinase C zeta. It contains an actin binding site and an ATP binding site and is most closely related to twinfilin (PTK9), a conserved actin monomer binding protein. TWF2 is an actin binding protein involved in motile and morphological processes. It inhibits actin polymerization, likely by sequestering G actin. By capping the barbed ends of filaments, it also regulates motility. It seems to play an important role in clathrin mediated endocytosis and distribution of endocytic organelles.
Subunit : Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF1 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down-regulated. Interacts with MYO7A
Subcellular Location : Cytoplasmic, cytoskeleton. Cytoplasmic, perinuclear region. Note: Perinuclear and G actin rich cortical actin structures sublocalization.
Tissue Specificity : Ubiquitously expressed (at protein level).
Post-translational modifications : In vitro, phosphorylated by PRKCZ, CK2 and SRC.
Similarity : Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.
Contains 2 ADF-H domains.
Database links : UniProtKB/Swiss-Prot: Q6IBS0.2
AFP ELISA Kit 大鼠甲種胎兒球蛋白/甲胎蛋白Multi-class antibodies規(guī)格: 48T
單抗 單抗Multi-class antibodies規(guī)格:
Rhesus antibody Rh FRMD1 FRMD1蛋白抗體 規(guī)格 0.2ml
AGEs ELISA Kit 大鼠晚期糖基化終末產(chǎn)物 96T
Neurofascin 英文名稱: 神經(jīng)束蛋白抗體 0.1ml
AHNAK 英文名稱: 神經(jīng)細胞分化相關(guān)蛋白AHNAK抗體 0.1ml
單抗 單抗Multi-class antibodies規(guī)格:
phospho-FAK(pTyr577) 磷酸化粘著斑激酶抗原Multi-class antibodies規(guī)格: 0.5mg
橋連整合因子蛋白抗體 Anti-BIN1 0.1ml
Rhesus antibody Rh Phospho-EEF2 (Thr56) 磷酸化真核翻譯延長因子2抗體 規(guī)格 0.1ml
品名 規(guī)格 備注
ZNF342 英文名稱: 鋅指蛋白342抗體 0.2ml
phospho-DAP Kinase 1 (Ser 308) 英文名稱: 磷酸化凋亡相關(guān)蛋白激酶1抗體 0.1ml
橋連整合因子蛋白抗體 Anti-BIN1 0.1ml
Anti-phospho-FOXO4 (phospho S197) /FITC 熒光素標記兔抗人、大、小鼠叉頭蛋白4抗體IgGMulti-class antibodies規(guī)格: 0.2ml
Anti-NKR/Neurokin B receptor /FITC 熒光素標記神經(jīng)激肽-B受體抗體IgGMulti-class antibodies規(guī)格: 0.2ml
Rhesus antibody Rh BTBD1/HCV NS5A transactivated protein 8 丙毒NS5A反轉(zhuǎn)錄蛋白8抗體 規(guī)格 0.2ml
ASCL1/MASH1(Achaete-scute homolog 1) 神經(jīng)母細胞特異性轉(zhuǎn)移因子抗原 0.5mg
Acetyl-Histone H4(K8) 英文名稱: 乙酰化組蛋白H4抗體 0.1ml
Rhesus antibody Rh RIPK3 受體結(jié)合絲氨酸蘇氨酸激酶3抗體 規(guī)格 0.1ml
Anti-NKR/Neurokin B receptor /FITC 熒光素標記神經(jīng)激肽-B受體抗體IgGMulti-class antibodies規(guī)格: 0.2ml
人型肝炎IgM(HCV-IgM)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝
豚鼠白介素5(IL-5)免疫試劑盒 Guinea pig ierleukin 5,IL-5 ELISA Kit
髓磷脂堿性蛋白(MBP)ELISA試劑盒 ,英文名: MBP ELISA Kit
MouseVitaminB6,VB6ELISA試劑盒小鼠維生素B6(VB6)ELISA試劑盒規(guī)格:96T/48T
FishOsteocalcin/Boneglaprotein,OT/BGPELISA試劑盒魚骨鈣素/骨谷氨酸蛋白(OT/BGP)ELISA試劑盒規(guī)格:96T/48T
HumanankyrinB,Ank-BELISAKit人錨蛋白B(Ank-B)ELISA試劑盒規(guī)格:96T/48T
大鼠同型半胱酸(HA)ELISA試劑盒 ,英文名: HA ELISA Kit
Bovine glucose 6 phosphate dehydrogenase (G6PD) ELISA Kit 牛葡萄糖6磷酸脫氫酶(G6PD)ELISA試劑盒
RatNeonatalThyroxine,NN-T4ELISAKit 大鼠新生甲狀腺素(NN-T4)ELISA試劑盒 96T/48T 進口分裝
CLIAKitforHO-2(Humanhemeoxygenase2)ELISAKit人血紅素氧合酶2規(guī)格:48T/96T
細胞PKA激酶活性定量檢測試劑盒(A/B/C)20/50次
ELISAKitP-Selectin/CD62P大鼠P選擇素規(guī)格:48T/96T
細胞骨架結(jié)合蛋白2抗體說明書 大鼠纖維蛋白肽B(FPB)ELISA試劑盒 ,英文名: FPB ELISA Kit
Mouse chorionic gonadoopin (CG) ELISA Kit 小鼠絨毛膜激素(CG)ELISA試劑盒
Mousemaixmetalloproteinase5,MMP-5ELISAKit 小鼠基質(zhì)金屬蛋白酶5(MMP-5)ELISA試劑盒 96T/48T 進口分裝
CLIAKitforHuman2,3-dinohromboxaneB2,2,3-dinor-TXB2ELISAKit人2,3Dinor烷B2規(guī)格:48T/96T
細胞HSV(HERPESSIMPLX)病毒定性檢測試劑盒20次
ELISAKitEGF大鼠表皮生長因子規(guī)格:48T/96T
抗體的生物素化標記實驗要點:
1. 細胞骨架結(jié)合蛋白2抗體說明書 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析;
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件;
3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導致抗體失活;
4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等;
5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性;
6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合;
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用;
8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導致高本底。
抗體的鑒定:
1)細胞骨架結(jié)合蛋白2抗體說明書 抗體的效價鑒定:不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。
2)抗體的特異性鑒定:抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力??贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3)抗體親和力:是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。